Sign Response Mechanism of TCA Self-assembled Fluorescence Probe for Cu~(2+) Detection: FRET Evidence by DFT

A new type of self-assembled molecule ON-OFF fluorescence probe for toxic transition metal ions, made up of thiacalix[4]arene, micelle and fluorescence group, has been studied by DFT/TDDFT method combined with experiment spectra. Since the mechanism of the optical quenching signal response of such self-assembled micelle probe has always been a controversial issue of uncertainty, the spatial construction and geometric structures of the functional units of probe in the Cu~(2+) ion detecting process were calculated and the mechanism was investigated by the molecular transition orbital pairs method to explore the origination of ON-OFF fluorescence sign response. The results presented that the signal response mechanism of the micelle probe is ascribed to F?rster resonance energy transfer(FRET) which provides new sights different from most of the conclusions by the related research work reported.     

Dual-core photonic crystal fiber polarization splitter based on lead silicate glass

Integral imaging is a three dimensional(3D)display technology without any additional equipment.A new system is proposed in this paper which consists of the elemental images of real images in real mode(RIRM)and the ones of virtual images in real mode(VIRM).The real images in real mode are the same as the conventional integral images.The virtual images in real mode are obtained by changing the coordinates of the corresponding points in elemental images which can be reconstructed by the lens array in virtual space.In order to reduce the spot size of the reconstructed images,the diffuser in conventional integral imaging is given up in the proposed method.Then the spot size is nearly1/20 of that in the conventional system.And an optical integral imaging system is constructed to confirm that our proposed method opens a new way for the application of the passive 3D display technology.     

大功率LED散热用微通道铝基板的有限元仿真

对带微通道的铝基板上封装的不同功率LED,用Comsol Multiphysics软件对其温度场进行了有限元模拟,重点研究了微通道的孔大小、孔间距、绝缘层的厚度和热导率对基板散热性能的影响,结果表明:铝基板厚度为1.5mm左右,微通道方形孔,孔长0.8mm,孔间距0.8mm,绝缘层厚度50μm,热导率1.5 W/(m·K),为最佳散热性能铝基板.微通道铝基板封装3W灯珠与普通铝基板和氮化铝基板相比,热阻分别下降了5.44和3.21℃/W,表明微通道铝基板能更好地满足大功率LED散热的需求.     

低温条件下光学厚度对Tm3+：YAG材料光谱烧孔孔深的影响

通过理论与实验研究了光学厚度对Tm3+：YAG材料光谱烧孔孔深的影响。提出了一种用于分析光学厚度对光谱烧孔孔深影响的新模型。该模型从理论上推导了烧孔孔深与光学厚度的关系。根据提出的理论模型,当温度大于4K时,通过选择合适的光学厚度可以使光谱烧孔孔深得到最大值。最后通过使用合适的激光与Tm3+：YAG材料所形成的光谱烧孔实验证明了实验结果与理论分析是相一致的。     

计算机病毒析理及防范

计算机病毒通常由计算机操作系统的弱点传播,提高系统的安全性是一个重要的抗病毒的方面.但那是没有完美的系统,过分强调提高系统的安全性,会使系统的大部分时间都在检查这种病毒是否存在,系统就失去了可用性、实用性和易用性的一方面,信息保密的要求使它无法选择泄漏,抓住这种病毒是重要的。     

雷达伺服性能在线自动测试的面向对象设计

雷达伺服性能的在线自动测试对系统调试和维护具有十分重要的意义。文中在认真梳理雷达伺服性能测试方法的基础上,采用面向对象设计方法实现了在线自动测试。经多套伺服系统的在线实际验证,该测试模块使用方便,可信度高,具有很高的工程应用价值。     

基于核酸分子杂交的免疫芯片抗体固定新方法

基于核酸分子杂交原理构建了一种新型抗体固定方法.先将抗体与寡核苷酸单链交联,再将两者的复合物与固相载体表面上的互补寡核苷酸链结合,从而将抗体固定到载体的表面.在磁珠表面对该固定方法进行实验,证明了方法的可行性.以本方法构建了针对转基因Bt Cry1Ac蛋白的免疫芯片,用Cy3标记二抗对其探针固定效果进行分析,并且在芯片上对Bt Cry1 Ac蛋白进行梯度浓度检测试验.结果表明,以本方法构建的抗体芯片,探针分布具有良好的特异性;探针层分布均匀,非特异吸附小;检测灵敏度达到0.01 ～ 0.05 μg/L;此外,通过杂交核酸双链的解离成功实现了芯片的再生,有助于解决传统抗体固定方法中芯片不可再生的问题.     

吸收液采样-顶空-气相色谱法测定废气中吡啶

用吸收液采集废气样品,顶空进样,采用气相色谱法测定进样气体中吡啶的含量。用DB-FFAP毛细管色谱柱分离,氢火焰离子化检测器测定。优化的试验条件如下:①采用多孔玻板吸收瓶采集样品;②吸收液的体积为45mL;③采样流量为0.5L·min^-1;④样品在4℃下,7d内完成分析;⑤吸收液的pH大于12;⑥加入3g氯化钠调节吸收液离子强度;⑦顶空温度为80℃,顶空平衡时间为30min。吡啶的质量浓度在100mg·L^-1以内与其对应的峰面积呈线性关系,检出限为0.05 mg·m^-3。对3个不同浓度水平的吡啶标准气体进行测定,相对误差为-8.0%^-4.7%,测定值的相对标准偏差(n=6)为1.3%~7.8%。     

Trajectory equation of a lump before and after collision with other waves for generalized Hirota–Satsuma–Ito equation

Based on the Hirota bilinear and long wave limit methods, the hybrid solutions of m-lump with n-soliton and nbreather wave for generalized Hirota–Satsuma–Ito(GHSI) equation are constructed. Then, by approximating solutions of the GHSI equation along some parallel orbits at infinity, the trajectory equation of a lump wave before and after collisions with n-soliton and n-breather wave are studied, and the expressions of phase shift for lump wave before and after collisions are given. Furthermore, ...