Identification of the Enzymes Mediating the Maturation of the Seryl‐tRNA Synthetase Inhibitor SB‐217452 during the Biosynthesis of Albomycins

Albomycin δ₂ is a sulfur‐containing sideromycin natural product that shows potent antibacterial activity against clinically important pathogens. The l ‐serine‐thioheptose dipeptide partial structure, known as SB‐217452, has been found to be the active seryl‐tRNA synthetase inhibitor component of albomycin δ₂. Herein, it is demonstrated that AbmF catalyzes condensation between the 6′‐amino‐4′‐thionucleoside with the d ‐ribo configuration and seryl‐adenylate supplied by the serine adenylation activity of AbmK. Formation of the dipeptide is followed by C3′‐epimerization to produce SB‐217452 with the d ‐xylo configuration, which is catalyzed by the radical S‐adenosyl‐l ‐methionine enzyme AbmJ. Gene deletion suggests that AbmC is involved in peptide assembly linking SB‐217452 with the siderophore moiety. This study establishes how the albomycin biosynthetic machinery generates its antimicrobial component SB‐217452.     

Simulation study of poled low-water ionomers with different architectures

The role of the ionomer architecture in the formation of ordered structures in poled membranes is investigated by molecular dynamics computer simulations. It is shown that the length of the sidechain L(s) controls both the areal density of cylindrical aggregates N(c) and the diameter of these cylinders in the poled membrane. The backbone segment length L(b) tunes the average diameter D(s) of cylindrical clusters and the average number of sulfonates N(s) in each cluster. A simple empirical formula is noted for the dependence of the number density of induced rod-like aggregates on the sidechain length L(s) within the parameter range considered in this study.     

4H-3,1-苯并噁嗪-4-酮衍生物的合成及α-葡萄糖苷酶抑制活性研究

为了探索高效低毒的新型α-葡萄糖苷酶抑制剂,设计并合成了一系列4H-3,1-苯并噁嗪-4-酮衍生物,其结构经过¹H NMR、¹³C NMR和HRMS鉴定。体外α-葡萄糖苷酶抑制活性测定结果表明,化合物3a、4a、5a和4j的IC₅₀值分别为68.3、76.1、99.3和51.9μmol/L,明显优于阳性对照阿卡波糖(IC₅₀=421.7μmol/L)。此外,分子对接研究结果表明化合物3a和4j与α-葡萄糖苷酶存在较强的亲和力。     

Synthesis and Insecticidal Activities of cis‐Configuration Nitenpyram Analogues with Benzoyl Hydrazines

To research on the structure–activity relationships of our designed neonicotinoid compounds, a series of novel cis‐configuration nitenpyram analogues with benzoyl hydrazines were designed and synthesized. The structures of all compounds were confirmed by IR, ¹H NMR, MS, and elemental analysis. Preliminary bioassays indicated that all the analogues exhibited good insecticidal activities against Nilaparvata legen and Aphis medicagini at 500 mg L^?1.     

非线性左右手媒质界面的Goos-Hänchen位移

 由均匀平面电磁波在左右手媒质界面满足的切向边界条件出发,推导了电磁波由线性传统媒质入射到非线性左手媒质时波的传播特性。利用时间延迟的方法,给出全反射情况下媒质界面非线性Goos-Hänchen位移表达式。分析了非线性左手媒质界面的侧向位移随入射角及入射波电场强度的变化关系,发现入射波场强对传输特性起决定作用:当入射波电场小于临界场强时,调节入射场强可以控制相应的侧向位移;当入射波电场大于临界场强时,不再满足全反射条件,部分入射波透射到非线性介质中。波导中加入非线性介质不仅可以调节侧向位移的大小,且可以实现对入射波场强的控制。     

Colloidal crystal growth at externally imposed nucleation clusters

We study the conditions under which and how an imposed cluster of fixed colloidal particles at prescribed positions triggers crystal nucleation from a metastable colloidal fluid. Dynamical density functional theory of freezing and Brownian dynamics simulations are applied to a two-dimensional colloidal system with dipolar interactions. The externally imposed nucleation clusters involve colloidal particles either on a rhombic lattice or along two linear arrays separated by a gap. Crystal growth occurs after the peaks of the nucleation cluster have first relaxed to a cutout of the stable bulk crystal.     

Dynamical density functional theory with hydrodynamic interactions and colloids in unstable traps

A density functional theory for colloidal dynamics is presented which includes hydrodynamic interactions between the colloidal particles. The theory is applied to the dynamics of colloidal particles in an optical trap which switches periodically in time from a stable to an unstable confining potential. In the absence of hydrodynamic interactions, the resulting density breathing mode exhibits huge single peaked oscillations in the trap center which become double peaked and damped by hydrodynamic interactions. The predicted dynamical density fields are in good agreement with Brownian dynamics computer simulations.     

Influence of hydrodynamic interactions on lane formation in oppositely charged driven colloids

The influence of hydrodynamic interactions on lane formation of oppositely charged driven colloidal suspensions is investigated using Brownian dynamics computer simulations performed on the Rotne-Prager level of the mobility tensor. Two cases are considered, namely sedimentation and electrophoresis. In the latter case the Oseen contribution to the mobility tensor is screened due to the opposite motion of counterions. The simulation results are compared to that resulting from simple Brownian dynamics where hydrodynamic interactions are neglected. For sedimentation, we find that hydrodynamic interactions strongly disfavor laning. In the steady state of lanes, a macroscopic phase separation of lanes is observed. This is in marked contrast to the simple Brownian case where a finite size of lanes was obtained in the steady state. For strong Coulomb interactions between the colloidal particles a lateral square lattice of oppositely driven lanes is stable similar to the simple Brownian dynamics. In an electric field, on the other hand, the behavior is found in qualitative and quantitative accordance with the case of neglected hydrodynamics.     

平面行波场中多个粒子受到的声辐射力*

从粒子在行波场中的声散射出发,研究当声场中存在稀疏分布的多个粒子时粒子受到的声辐射力,并且给出了适用于声场中任意位置的粒子声辐射力计算公式。由于声辐射力为非线性力,当声场中存在多个粒子时,直接计算粒子受到的声辐射力非常复杂。结果表明,当声场中存在多个稀疏分布的粒子时,这一多粒子系统可以视为多个单独的双粒子系统的叠加,只需要分别计算各个双粒子系统的声辐射力就可以通过叠加得到声场中任意粒子的声辐射力。这一结果有助于利用声辐射力对微小粒子进行精细操控。