Preparation of Antibacterial Gelatin/Genipin Nanofibrous Membrane for Tympanic Membrane Repair

Tympanic membrane perforation (TMP), a common disease, often needs a scaffold as the patch to support surgery. Due to the environment of auditory meatus, the patch can be infected by bacteria that results in failure; therefore, the ideal scaffold may combine biomimetic and antibacterial features. In this work, gelatin was used as the electrospinning framework, genipin as the crosslinking agent, and levofloxacin as an antibacterial in order to prepare the scaffold for TMP. Different contents of levofloxacin have been added to gelatin/genipin. It was found that, with the addition of levofloxacin, the gelatin/genipin membranes exhibit improved hydrophilia and enhanced tensile strength. The antibacterial and cell-cultured experiments showed that the prepared antibacterial membranes had excellent antibacterial properties and good biocompatibility, respectively. In summary, levofloxacin is a good group for the gelatin/genipin scaffold because it improves the physical properties and antibacterial action. Compared with different amounts of levofloxacin, a gelatin/genipin membrane with 1% levofloxacin is more suitable for a TM.     

利用光子晶体光纤产生超连续飞秒激光光谱

将单脉冲能量约为2．5nJ、脉宽为25fs、对应峰值功率为0．1MW的的800nm钛宝石激光耦合到长为10mm，芯径为1．8μm的光子晶体光纤中，产生了耦合效率约为17％、谱宽覆盖可见光及近红外波段的超连续光谱。     

Fabrication of Single-Phase BaTi4O9 Nanocrystalline Powder by Sol-Gel Process

BaTi₄O₉ nanocrystalline powder was prepared by sol-gel method using Ti(OC₄H₉)₄ and Ba(CH₃COO)₂ as raw materials. The optimum process was obtained by analyzing the synthesis condition of the single-phase BaTi₄O₉ nanocrystalline powder as follows: the content of acetyl-Titanium = 1 mol/L. pH = 4.2, molar ratio of water/alkoxide = 15, and the powder is kept at 1200°C for 2 h. The XRD and TEM analysis showed that the single-phase BaTi₄O₉ nanocrystalline powder of 30 nm in size was well prepared.     

Visual snapshots of intracellular kinase activity at the onset of mitosis

Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC beta isoform with subsequent NEB.     

液晶叶绿素铂电极的光感电位

将不同含量的液晶物质4-甲氧基苄叉4′-正丁基苯胺(MBBA)分别加到叶绿素a(Chla)的异辛烷溶液中, 将这些溶液分别涂在铂板上形成液晶叶绿素铂电极, 测求其光感电位ΔE的变亿; 另一方面将这些溶液分别涂在玻璃板上, 形成液晶叶绿素涂层的玻璃板, 测求其导数光谱特性谱峰(743 nm)相对高度h/h_T的变化。发现随着MBBA量的增加, ΔE与h/h_T两种数值均有极大信出现。另外用石油醚溶剂、异辛烷-石油醚(1:9V/V)溶剂代替异辛烷溶剂, 进行同样实验, 发现也都有这两种极大值出现, 而且这三种溶剂体系的六个极大值都发生在MBBA浓度为0.001±0.0005 mol L~(-1)范围内。本文根据导数光谱原理, 子结构以及液晶物质特性, 对以上实验的变化规律作了理论说明。     

海洋高光谱辐射实时观测系统的研制

研制了海洋水色高光谱辐射实时观测系统.该系统主要包括用于现场测量海面与海水近表层上行辐亮度和下行辐照度的高光谱光纤辐射计,装载高光谱光纤辐射计及其它辅助设施的海上观测平台,数据采集、实时通讯的控制系统.采用6通道光纤光谱仪解决了多参数测量同步性问题,通过自动调整光谱仪CCD积分时间提高其测量的动态范围;采用光纤光谱仪减小了光接收器的体积,减小了自阴影效应的影响;采用防污染装置解决了光学探头水下防污染问题.对系统的性能进行了室内测试及近海36 d试验.结果表明,光学系统零漂误差小于±5%,光学系统稳定可靠;浮标性能可很好地满足水下光辐射测量对浮标体姿态和稳定性的要求;控制系统的数据采集和通讯可靠有效.     

微生物批式流加发酵多阶段最优控制的最优性条件

在微生物批式流加发酵生产1,3一丙二醇(1,3-PD)过程中,关键是如何控制甘油和碱的流加速度.本文将流加速度看成一个随时间变化的控制函数,提出一个带控制的多阶段动力系统描述批式发酵过程,并证明了系统的一些性质.以终端时刻1,3-PD的生产强度最大为性能指标,以上述动力系统和连续状态不等式为约束条件建立了最优控制模型,最后利用不可微优化理论得到了最优控制问题的最优性条件,并证明了最优性条件和最优性函数零点的等价性.     

5H-3-oxa-Octafluoropentanesulfonyl fluoride: a novel and efficient condensing agent for esterification, amidation and anhydridization

The use of 5H-3-oxa-octafluoropentanesulfonyl fluoride (HCF₂CF₂OCF₂CF₂SO₂F) as a novel and efficient condensing reagent for esterification of carboxylic acids with alcohols and amidation of carboxylic acids with amines in the presence of 1,3-diazabicyclo[5.4.0]-undec-7-ene (DBU) is reported. HCF₂CF₂OCF₂CF₂SO₂F cannot serve as a condensing agent for anhydridization of carboxylic acids, however, HCF₂CF₂OCF₂CF₂SO₂F/(CH₃)₃SiCN system can mediate anhydridization of some aromatic carboxylic acids.     

b→sγ在Multiscale Walking Technicolor模型中的研究

研究Multiscale Walking Technicolor模型(MWTCM)中赝标戈尔斯通玻色子(PGB)对b→sγ分支比的修正,发现修正太大以致很难解释最近CLEO的实验数据,如果进一步引入Topcolor,在Topcolor assistedMWTCM中,BR(b→sγ)在参数的一定空间内同CLEO的实验数据一致.     

Altered Phase Interactions between Spontaneous Blood Pressure and Flow Fluctuations in Type 2 Diabetes Mellitus: Nonlinear Assessment of Cerebral Autoregulation

Cerebral autoregulation is an important mechanism that involves dilatation and constriction in arterioles to maintain relatively stable cerebral blood flow in response to changes of systemic blood pressure. Traditional assessments of autoregulation focus on the changes of cerebral blood flow velocity in response to large blood pressure fluctuations induced by interventions. This approach is not feasible for patients with impaired autoregulation or cardiovascular regulation. Here we propose a newly developed technique—the multimodal pressure-flow (MMPF) analysis, which assesses autoregulation by quantifying nonlinear phase interactions between spontaneous oscillations in blood pressure and flow velocity during resting conditions. We show that cerebral autoregulation in healthy subjects can be characterized by specific phase shifts between spontaneous blood pressure and flow velocity oscillations, and the phase shifts are significantly reduced in diabetic subjects. Smaller phase shifts between oscillations in the two variables indicate more passive dependence of blood flow velocity on blood pressure, thus suggesting impaired cerebral autoregulation. Moreover, the reduction of the phase shifts in diabetes is observed not only in previously-recognized effective region of cerebral autoregulation (<0.1 Hz), but also over the higher frequency range from ∼0.1 to 0.4 Hz. These findings indicate that type 2 diabetes mellitus alters cerebral blood flow regulation over a wide frequency range and that this alteration can be reliably assessed from spontaneous oscillations in blood pressure and blood flow velocity during resting conditions. We also show that the MMPF method has better performance than traditional approaches based on Fourier transform, and is more suitable for the quantification of nonlinear phase interactions between nonstationary biological signals such as blood pressure and blood flow.