2-取代亚肼基-1,3-二硫杂环戊烷类化合物的合成及其生物活性研究

以取代苯甲醛、取代吡啶和取代苯甲酸酯等为原料合成了 10种含硫杂环化合物 (其中 2a～ 2g化合物为国内外未见报道 ) .在合成过程中采用了超声波技术 ,极大地缩短了反应时间 ,提高了收率 .所得化合物的结构经IR ,1 HNMR ,MS和元素分析证实 .对其生物活性进行了初步测试 ,结果证明一些化合物具有良好的抑菌或杀菌作用 ,其中 2b ,2c ,2e ,2h ,2i和2j对大肠杆菌 ( 10 40 7号 )的抗菌效果与CET和SMZ相当 ;2b ,2c ,2f对草绿色链球菌 ( 112 3号 )的抗菌效果与CET和SMZ相当 .     

二碘化钐促进的硒代酯的合成

用SmI2/THF/HMPA体系在室温下使二芳基二硒醚中的Se-Se键还原断裂成硒负离子物种, 继而与酸酐作用得到硒代酯, 该反应条件温和中性, 产率良好, 提供了合成硒代酯的一个新途径。     

广州黄埔区内0～6岁儿童近五年健康状况分析

为了解最近5年广州医学院港湾医院辖区内0~6岁小儿的营养、健康状况变化趋势和明确今后儿童保健工作的重点,对该院管辖的7所幼儿园集体儿童及9个居委散居儿童,连续5年(2002—2006年)的体检资料进行了回顾性分析,人数达14485人,所有的体检资料均按照《WHO 0~6岁儿童身高体质量参考值及评价标准》以及贫血、视力异常、龋齿的国家卫生部标准进行评价。结果表明,总体的儿童营养不良率为0.86%,肥胖率为1.87%,生长迟缓患病率为1.26%,轻度贫血率1.35%;观察各年情况,肥胖率有逐年增长趋势,儿童营养不良率无逐年下降趋势;广州医学院港湾医院辖区内0~6岁小儿的营养不良、肥胖、生长迟缓、轻度贫血等患病率低于全国水平,但也有不容忽视之处,营养不良、轻度贫血等并没有因生活水平的提高而消失。加强对家长的科学育儿健康教育,以提高他们的育儿知识水平,是儿保工作的重点,应进一步降低儿童营养不良、贫血的发生率;对儿童肥胖应当切实采取措施,预防和控制肥胖症的发生。     

混合溶剂对磺化聚苯乙烯水基微乳液体系形态的影响

利用相反转技术制备磺化聚苯乙烯(SPS)水基微乳液,研究发现,通过选择溶剂和控制混合溶剂的组成,可制备不同颗粒形态结构的SPS水基微乳液,并研究了相反转过程、乳液稳定性与混合溶剂组成之间的关系。     

Polyethylenimine （PEI）-g-comb-poly（ethylene glycol）-transferrin（Ⅰ）： Tumor-targeted Vector for Gene Delivery In.vitro

The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa. Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5 （mol/mol）. The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.     

Application of capillary electrophoretic immunoassay to analysis of estradiol in women's serum

Summary A rapid and sensitive capillary electrophoretic immunoassay is described for determination of estradiol in women's serum. Addition of thermally reversible hydrogel in the buffer, serving as a replaceable packing material, improved the reproducibility of the method. Using a laser-induced fluorescence detector this method can be applied to the determination of estradiol at concentrations as low as 30.6 pg mL¹. Estradiol levels in 16 normal women's serum were measured at the range 115≈370 pg mL¹. The results of this method have been found to correlate well with those of chemiluminescent immunoassay.     

Intramolecular proton transfer induced by divalent alkali earth metal cation in the gas state

Interactions between divalent alkali earth metal (DAEM) ions M (M?Be, Mg, Ca, Sr, Ba) and the second stable glycine conformer in the gas phase, which can transfer into the ground‐state glycine‐M²⁺ (except the glycine–Be²⁺) among each corresponding isomers when these divalent metal ions are bound, are studied at the hybrid three‐parameter B3LYP level with three different basis sets. Proton transfers from the hydroxyl to the amino nitrogen of the glycine without energy barriers have been first observed in the gas phase in these glycine–M²⁺ systems. The interaction between the glycine and these DAEM ions except beryllium and magnesium ion only create an amino hydrogen pointing to the original hydroxyl due to their weaker interaction relative to those divalent transition metal (DTM) ion‐bound glycine derivatives, being obviously different from that between the glycine and DTM ions, in which two amino hydrogens point to the original hydroxyl oxygen when these metal‐chelated glycine derivatives are produced. The interaction energy between the glycine and divalent magnesium would be the boundary of one or two amino hydrogens pointing to the hydrogyl oxygen, i.e., the ?170.3 kcal/mol of binding energy is a critical point. Similar intramolecular proton transfer has also been predicted for those DTM ion‐chelated glycine systems; however, that in the gas state has not been observed in the monovalent metal ion‐coordinated glycine systems. The binding energy between some monovalent TM ion and the glycine is similar to that of the glycine–Ba²⁺, which has the lowest binding strength among these DAEM–ion chelated glycine complexes. The difference among them only lies in the larger electrostatic and polarized effects in the latter, which favor the stability of the zwitterionic glycine form in the gas phase. According to these observations, we predict that the zwitterionic glycine would exist in the field of two positive charges in the gas phase. © 2003 Wiley Periodicals, Inc. Int J Quantum Chem 94: 205–214, 2003     

Formation of ring calcium oxalate patterns induced by domains in DPPC Langmuir-Blodgett films

The ring patterns of calcium oxalate crystals were induced by domains in Langmuir-Blodgett （LB） films of dipalmitoylpho- sphatidylcboline （DPPC）. The result was explained by the defects at the ring boundaries of liquid condensed （LC） and liquid expanded （LE） phases of LB film. These boundaries could provide less free energy and much more nucleating sites for COM crystals.     

Weighted two-band target entropy minimization for the reconstruction of pure component mass spectra: Simulation studies and the application to real systems

A method is proposed, on the basis of a recently developed algorithm--Band Target Entropy Minimization (BTEM)--to reconstruct mass spectra of pure components from mixture spectra. This method is particular useful in dealing with spectral data with discrete features (like mass spectra). Compared to the original BTEM, which has been applied to differentiable spectroscopies such as Fourier-transfer infrared spectroscopy (FTIR), ultraviolet (UV), Raman, and nuclear magnetic resonance (NMR), the latest modifications were obtained through: (1) Reformulating the objective function using the peak heights instead of their derivatives; (2) weighting the abstract vector VT to reduce the effect of noise; (3) using a two-peak targeting strategy (tBTEM) to deal with strongly overlapping peaks; and (4) using exhaustive search to locate all the component spectra. A set of 50 multi-component mass spectra was generated from ten reference experimental pure component spectra. Many of the compounds chosen have common MS fragments and therefore, many of the pure component spectra have considerable intensity in same data channels. In addition, a set of MS spectra from a real system with four components was used to examine the newly developed algorithm. Successful reconstruction of the ten component spectra of the simulated system and the four component spectra of the real system was rapidly achieved using the new tBTEM algorithm. The advantages of the new algorithm and its implication for rapid system identification of unknown mixtures are readily apparent.