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61.
In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells. 相似文献
62.
A diarylethene derivative bearing a phenylenediamine group formed radical ions with an electron acceptor molecule in solution, and the concentration of the radical ions was modulated by the photochromic reaction of the diarylethene, reflecting the difference in the electron-donating character between the open- and closed-ring isomers. 相似文献
63.
Uchida K Walko M de Jong JJ Sukata S Kobatake S Meetsma A van Esch J Feringa BL 《Organic & biomolecular chemistry》2006,4(6):1002-1006
Upon UV irradiation of the hydrogen-bond confined crystal state of a dithienylhexafluorocyclopentene with (R)-N-phenylethylamide substituents, the photochemical cyclization reaction proceeds diastereoselectively to form the coloured, closed-ring isomer with 97% de. 相似文献
64.
A PVC-based anion-selective electrode was constructed with use of Capriquat as ion-exchanger. Experimental data were in good agreement with E = E(0) - (RT/F)ln(C + radicalC (2) + A (x))/2. The parameter A(x) governs the limit of detection. It was experimentally shown that A(x) was related to the selectivity coefficient K(xy) by K(xy) = A(y)/A(x). 相似文献
65.
Single-crystalline photochromism of 1,2-bis(2-methyl-5-phenyl-3-thienyl)perfluorocyclopentene 1a was followed in situ by X-ray crystallographic analysis. The crystal of 1a has two molecules with different conformations in the crystallographic asymmetric unit. The X-ray analysis of the photoirradiated crystal showed that the photocyclization reaction of the molecule with the shorter distance between the reactive carbons prevails over the reaction of the other molecule. The mechanism has been discussed based on the calculation of the electronic transitions of the two molecules in the different conformations. The photocyclization quantum yield was determined to be 1 (100%) in the crystal. 相似文献
66.
Ayako Torikai Takahiro Kobatake Fumio Okisaki Hideo Shuyama 《Polymer Degradation and Stability》1995,50(3):261-267
Wavelength dependence on the photodegradation of flame-retardant materials (Polystyrene (PSt) containing flame-retardants) was studied. The effects of nine flame-retardants containing bromine (Br) on the efficiency of photodegradation of the PSt were compared. Samples were irradiated at 23 °C in air with monochromatic radiation of wavelengths 260, 280, 300, 320, 340, 360 and 380 nm using the Okazaki Large Spectrograph (OLS). UV-visible and FTIR spectra were measured to identify photo-induced chemical structural changes in the PSt. The number of main-chain scissions and the changes in the molecular weight distribution were estimated from the results of GPC measurements. It was found that the photo-stability of PSt was reduced by the addition of flame-retardants. The most effective irradiation wavelength for main-chain scission was found to be 280 nm for each flame-retardant except one. The photo-stability of PSt containing flame-retardants appears to depend upon the chemical structure of the additive. 相似文献
67.
Wada A Mie M Aizawa M Lahoud P Cass AE Kobatake E 《Journal of the American Chemical Society》2003,125(52):16228-16234
The chemically and genetically remodeling of proteins with ligand binding specificities can be utilized to synthesize various protein-based microsensors for detecting single biomolecules. Here, we describe the construction and characterization of fluorophore-labeled glutamine binding proteins (QBP) and derivatives coupled to the independently designed hydrophobic polypeptide (E12) that can adhere onto solid surfaces via hydrophobic interactions. The single cysteine mutant (N160C QBP) modified with the three environmentally sensitive fluorescent dyes (IAANS, acrylodan, and IANBD ester) showed increased changes in fluorescence intensity induced by glutamine binding. The use of these conjugates as reagentless fluorescence sensors enables us to determine the glutamine concentrations (0.1-50 microM) in homogeneous solution. The fusion of N160C QBP with E12, (Gly4-Ser)n spacers (GSn), and IANBD resulted in the novel fluorescence sensing elements having an adhering capability to hydrophobic surfaces of unmodified microplates. In ELISA and fluorescence experiments for the microplates treated with a series of the conjugates, IANBD-labeled N160C QBP-GS1-E12 displayed the best reproducibility in adhesion onto the hydrophobic surfaces and the precise correlation between fluorescence changes and glutamine concentrations. The performance of the biosensor-attached microplate for glutamine titrations demonstrated that the hydrophobic interaction of E12 with solid surfaces is useful for effective immobilization of proteins that need specific conformational movements in recognizing particular biomolecules. Therefore, the technique using E12 as a surface-linking domain for protein adhesion onto unmodified substrates could be applied effectively to prepare microplates/arrays for a wide variety of high-throughput assays on chemical and biological samples. 相似文献
68.
N Hazemoto S Ishizaka M Haga Y Kato S Kurosawa N Kamo Y Kobatake 《Chemical & pharmaceutical bulletin》1989,37(8):2153-2154
Ion-selective electrodes (ISEs) responsive to the antitussives dextromethorphan and dimemorfan were constructed by the fixation of an ion-exchanger, ammonium tetraphenylborate, on a Millipore membrane by means of a plasma-polymerization technique. The electrodes showed a Nernstian response over the range of 10(-5)-10(-2) M dextromethorphan and dimemorphan, and the working pH range was 5-7. The interference from common cations such as Na+, K+ and Ca2+ was negligible but some organic cations interfered weakly. The electrodes were applied successfully for the determination of the drugs in pharmaceutical preparations. 相似文献
69.
Tetsuo Ueda Yoshthito Mori Toshiyuki Nakagaki Yonosuke Kobatake 《Photochemistry and photobiology》1988,47(2):271-275
Abstract— Photoavoidance by plasmodia of an albino strain of Physarum polycephalum was studied. When the organism was irradiated locally, the protoplasm moved away from the irradiated region. The action spectrum for this avoidance showed three peaks at about 260, 370 and 460 nm. The organism was about one hundred times as sensitive to far UV as to near UV and blue light, and high intensity far-UV caused the gelation of the protoplasm. Irradiation with UV or blue light increased the mean level or the amplitude of oscillation in intracellular cAMP and cGMP concentrations. Upon UV irradiation, birefringent fibrils, presumably microfilaments of F-actin, became thick and numerous, and the plasmodial strand generated a strong tensile force. It is postulated that UV or blue light brings about an increased concentration of cyclic nucleotides which leads to an enhanced local development of contractile fibrils which squeeze protoplasmic sol from the area, resulting in photoavoidance. 相似文献
70.
Four polymorphic crystals were obtained by recrystallization of 1,2-bis(2-methyl-5-p-methoxyphenyl-3-thienyl)perfluorocyclopentene (1 a) from hexane. All crystals underwent photochromic reactions upon alternate irradiation with ultraviolet (lambda=370 nm) and visible light (lambda>500 nm). The photocyclization quantum yields were found to be close to unity irrespective of the crystal types, while the photocycloreversion quantum yields were different as much as four times depending on the conformation of the closed-ring isomers in the crystals. 相似文献