Examination of Template Structural Effects on CEC Chiral Separation Performance of Molecule Imprinted Polymers Made by a Generalized Preparation Protocol

Some acidic chiral templates were used to prepare open tubular (OT) molecule imprinted polymer (MIP) capillary columns to explore the effects of molecular structures of templates on chiral recognition capabilities and to verify the feasibility of the general MIP preparation protocol introduced in the previous study. The templates are phenyl carboxylic acids and their derivatives. Optimization was carried out for chiral separation of template enantiomers for each MIP column through varying pH and composition of eluent. It was found that the preparation protocol can be successfully applied for the appropriate templates with functional groups fulfilling the three-points interaction rule. The chiral separation performances were quite satisfactory for MIPs of such templates although they are yet inferior to the separation performances of the MIP columns fabricated with the templates of profen drugs (2-arylpropionic acids with a large substituent on the phenyl ring). Subtle variations of the template molecular structures have been found to be critical to enhance chiral recognition ability of the resultant MIP column.     

Dynamics of sprinkler jets

The problem of the motion of droplet-air sprinkler jets is examined. On the basis of the equations of a turbulent two-phase boundary layer the trajectories and range of these jets are calculated. A comparison of the calculation results with experiment indicates satisfactory agreement. It is shown that the expression for the range of a body projected at an angle to the horizontal gives exaggerated values for the range, while calculating the range for a single droplet with allowance for the air resistance gives values that are much too low. It has been established that in calculating the motion of sprinkler jets it is necessary to take into account the air resistance to the water droplets reduced as a result of the acceleration of the air in the immediate vicinity of the preceding droplets.Translated from Izvestiya Akademii Nauk SSSR, Mekhanika Zhidkosti i Gaza, No. 5, pp. 60–67, September–October, 1985.     

Flexural perturbations of free jets of maxwell and Doi-Edwards liquids

Flexural perturbations of high-velocity free jets of drop liquids moving in air are reinforced by the fact that the air pressure on the concave sections of the jet surface is greater than on the convex sections. The linear and nonlinear stages of development of flexural perturbations were studied in [1–5] for viscous Newtonian fluids. The effect of elastic stresses in the fluid on the growth of flexural perturbations of jets was first examined in [6], where it was assumed in an analysis of the growth of small disturbances that surface tension was constant along the jet, i.e., the investigators actually studied a tensed string. The studies [7, 8] examined the linear stage of growth of flexural perturbations of jets of Maxwell liquids. Our goal here is to analyze the dynamics of long-wave flexural perturbations of jets of viscoelastic fluids in both the linear and nonlinear stages of development. The rheological behavior of the fluid is described by two models — the phenomenological (Maxwell) model and the physical-molecular (Doi-Edwards) model. It is shown that the disturbances are oscillatory in character in the nonlinear stage of development. Meanwhile, the results of calculations performed with the Maxwell (M) and Doi-Edwards (DE) rheological models in the given problem agree with each other quantitatively as well as qualitatively.Translated from Zhurnal Prikladnoi Mekhaniki i Tekhnicheskoi Fiziki, No. 6, pp. 43–53, November–December, 1986.     

Unsteady jet flow of a viscous fluid

A solution is obtained, within the framework of the boundary layer theory, to the problem of the unsteady flow created by a two-dimensional jet source for a given momentum flux variation with time. In particular, aperiodic and periodic momentum variations are examined, and a qualitative analog with turbulent flow is noted for the latter.     

Enantioselective determination of modafinil in pharmaceutical formulations by capillary electrophoresis, and computational calculation of their inclusion complexes

A fast capillary electrophoretic method is described for the separation and determination of the enantiomers of the novel wake-promoting agent, modafinil. Several parameters affecting the separation were studied, including the type and concentration of chiral selector, buffer pH, buffer concentration, voltage and temperature. Good chiral separation of the racemic mixture was achieved in less than 5 min with resolution factor Rs?=?2.51, using a bare fused-silica capillary and a background electrolyte (BGE) of 25 mM H₃PO₄?1 M tris solution; pH 8.0; containing 30 mg mL^?1 of sulfated-β-cyclodextrin (S-β-CD). The separation was carried out in normal polarity mode at 25 ^?C, 18 kV and using hydrostatic injection. Acceptable validation criteria for selectivity, linearity, precision, and accuracy were included. The developed method was successfully applied to the assay of enantiomers of modafinil in pharmaceutical formulations. The computational calculations for the enantiomeric inclusion complexes rationalized the reasons for the different migration times between the modafinil enantiomers.     

On the Rayleigh-Plateau instability

In this paper,we study the surface instability of a cylindrical pore in the absence of stress. This instability is called the Rayleigh-Plateau instabilty. We consider the model developed by Spencer et ...     

Kinetic and analytical study on precipitation reactions with110AgNO3 of some di (β-chloroethyl) amine derivates and hydrochlorides with esters of N-(p-aminobenzoyl)-L-aspartic acid as carriers from dimethylformamide-water solution

The kinetics of precipitation reactions with¹¹⁰AgNO₃ of some di (β-chlorethyl) amine derivates and hydrochlorides with esters of N-(p-aminobenzoyl)-L-aspartic acid as carriers in dimethylformamide-water mixture, were studied. The rate constants of these reactions were of the order of 10^?4 1 · mol^?1 · min^?1. The concentrations of the corresponding hydrochloride solutions were measured by radiometric titration with¹¹⁰AgNO₃ solution of given concentration.     

Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry

Matrix-assisted laser desorption/ionization (MALDI) mass spectra were obtained from single biological aerosol particles using an aerosol time-of-flight mass spectrometer (ATOFMS). The inlet to the ATOFMS was coupled with an evaporation/condensation flow cell that allowed the aerosol to be coated with matrix material as the sampled stream entered the spectrometer. Mass spectra were generated from aerosol composed either of gramicidin-S or erythromycin, two small biological molecules, or from aerosolised spores of Bacillus subtilis var niger. Three different matrices were used: 3-nitrobenzyl alcohol, picolinic acid and sinapinic acid. A spectrum of gramicidin-S was generated from approximately 250 attomoles of material using a molar ratio of 3-nitrobenzyl alcohol to analyte of approximately 20:1. A single peak, located at 1224 Da, was obtained from the bacterial spores. The washing liquid and extract solution from the spores were analyzed using electrospray mass spectrometry and subsequent MS/MS product ion experiments. This independent analysis suggests that the measured species represents part of the B. subtilis peptidoglycan. The on-line addition of matrix allows quasi-real-time chemical analysis of individual, aerodynamically sized particles, with an overall system residence time of less than 5 seconds. These results suggest that a MALDI-ATOFMS can provide nearly real-time identification of biological aerosols. Copyright 2000 John Wiley & Sons, Ltd.