Alkali-hydroxide-doped matrices for structural characterization of neutral underivatized oligosaccharides by MALDI time-of-flight mass spectrometry

We report new approaches using alkali-hydroxide-doped matrices to facilitate structural characterization of neutral underivatized oligosaccharides by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) MS. The approaches involved pretreatment of the analytes with NaOH or LiOH in aqueous solution, followed by mixing them with MALDI matrices prior to MS analysis. It was found that for open-ended neutral underivatized oligosaccharides partial alkaline degradation occurred upon laser desorption and ionization of the hydroxide-pretreated analytes in 2,5-dihydroxybenzoic acid (DHBA). The effect intensified when nonacidic compounds such as 2,4,6-trihydroxyacetophenone (THAP) and 5-amino-2-mercapto-1,3,4-thiadiazole (AMT) were used as matrix. The degradation allowed facile identification of the reducing end residue of the analyte and facilitated its structural characterization by postsource decay TOF-MS. Applying the same technique using matrices composed of LiOH and THAP or AMT led to the production of singly as well as multiple lithiated ions of oligosaccharides containing hexoses with free 3-OH groups. Extensive lithiation through multiple hydrogen-lithium exchanges up to 6 Li atoms was observed for maltoheptaose, beta-cyclodextrin, and dextran 1500. Such a 'lithium tagging' technique makes it possible to differentiate positional isomers of milk-neutral oligosaccharides, lacto-N-difucohexaose I and II (LNDFH-I and LNDFH-II), without the need of chemical derivatization or tandem MS analysis.     

Identification of interrelationship of key customers’ needs based on structural model for services/capabilities provided by a Semiconductor-Intellectual-Property Mall

The semiconductor industry is shifting towards innovation and acquisition of intellectual property. Semiconductor-Intellectual-Property (SIP) design, a new industry, is also rapidly growing. This challenges both providers and users to develop infrastructure and standard interfaces. Establishing an SIP Mall to provide a full array of SIP business services is a new concept used to promote growth of the SIP industry. Many foundries and governments have been involved in setting up SIP Malls; however, the major services needed for an SIP Mall to attract SIP providers and SIP users must still be clarified. In this paper, the DEMATEL (DEcision MAking Trial and Evaluation Laboratory) method was used to discover and illustrate the key services needed to attract SIP users and SIP providers to an SIP Mall. Research enabled the derivation of the interrelated services and the structural interrelationship between them using the DEMATEL method. Overall, four key services were found to be vital for an SIP Mall to attract customers and to allocate resources efficiently.     

Normalized fifth-order spherical aberration coefficients derived from a Gaussian trajectory on rotationally symmetrical magnetic lenses

This paper reports normalized fifth-order spherical aberration coefficients C(5s)/R derived from Gaussian trajectory for rotationally symmetrical magnetic lenses, where S/D (the polepiece gap-width/bore-diameter ratio) is in the range 0.20-2.00, and k(2) (the lens-intensity parameter) is 0.10-100. It is noted that the normalized fifth-order spherical-aberration coefficient C(5s)/R changes its sign within the given parameter ranges.     

DEA models for minimizing weight disparity in cross-efficiency evaluation

Cross-efficiency evaluation is a commonly used approach for ranking decision-making units (DMUs) in data envelopment analysis (DEA). The weights used in the cross-efficiency evaluation may sometimes differ significantly among the inputs and outputs. This paper proposes some alternative DEA models to minimize the virtual disparity in the cross-efficiency evaluation. The proposed DEA models determine the input and output weights of each DMU in a neutral way without being aggressive or benevolent to the other DMUs. Numerical examples are tested to show the validity and effectiveness of the proposed DEA models and illustrate their significant role in reducing the number of zero weights.     

Combretastatin A-4 derivatives: synthesis and evaluation of 2,4,5-triaryl-1H-imidazoles as potential agents against H1299 (non-small cell lung cancer cell)

A number of 2,4,5-triaryl-1H-imidazole derivatives were synthesized and evaluated for their antiproliferative activities against the growth of five cell lines including three non-small cell lung cancers (H460, H1299, and A549), one breast cancer (MCF-7), and one normal diploid embryonic lung cell line (MRC-5). Preliminary results indicated that both 2-(5-bromofuran-2-yl)-4,5-bis{4-[3-(dimethylamino) propoxy] phenyl}-1H-imidazole (10f) and 4,5-bis{4-[3-(dimethylamino)propoxy]phenyl}-2-(5-nitrofuran-2-yl)-1H -imidazole (10g) were selectively active against the growth of H1229 with an IC₅₀ of less than 0.1???M, thus were more active than topotecan (IC₅₀ >?10.0??? M). However, both 10f and 10g exhibited only marginal cytotoxicity against H460, A549, MCF-7, and MRC-5 requiring an IC ₅₀ of at least 4.16???M. Our results also indicated that 10f induced H1299 cell cycle arrest at G0/G1 through the inactivation of p38 MAPK, JNK, ERK, as well as the expression of SIRT1 and survivin. These results suggested that 10f might have therapeutic potential against H1299 (non-small cell lung cancer cell).     

Optimal Control of a Ship for a Course-Changing Maneuver

The steering control of a ship during a course-changing maneuver is formulated as a Bolza optimal control problem, which is solved via the sequential gradient-restoration algorithm (SGRA). Nonlinear differential equations describing the yaw dynamics of a steering ship are employed as the differential constraints, and both amplitude and slew rate limits on the rudder are imposed. Two performance indices are minimized: one measures the time integral of the squared course deviation between the actual ship course and a target course; the other measures the time integral of the absolute course deviation. Numerical results indicate that a smooth transition from the initial set course to the target course is achievable, with a trade-off between the speed of response and the amount of course angle overshoot.     

Rough set-based logics for multicriteria decision analysis

In this paper, we propose some decision logic languages for rule representation in rough set-based multicriteria analysis. The semantic models of these logics are data tables, each of which is comprised of a finite set of objects described by a finite set of criteria/attributes. The domains of the criteria may have ordinal properties expressing preference scales, while the domains of the attributes may not. The validity, support, and confidence of a rule are defined via its satisfaction in the data table.