Purification and Characterization of Protein Tyrosine Phosphatase MEG1 and Preparation of Anti-PTPMEG1 Antibody

<正>PTPMEG1 is an intracellular protein tyrosine phosphatase(PTP),which contains FERM and PDZ domains. This study focuses our attention on the expression,purification and characterization of catalytic domain of PTPMEG1(△MEG1) and preparation of its polyclonal antibody.A cDNA fragment encoding△MEG1 protein(amino acid residues 643—926) was amplified by PCR and then cloned into the pT7-7 vector.Both soluble and insoluble recombinant△MEG1 proteins were observed after induction by IPTG.Soluble△MEG1 was purified via two chromatographic steps,and the purified enzyme was characterized.With para-nitrophenylphosphate(pNPP) as a substrate,△MEG1 exhibited typical enzymatic characteristics of classic PTPs and classical Michaelis-Menten kinetics.Insoluble△MEG1,which was mainly distributed in the inclusion body of E.coli cells extracts,was purified by preparative electrophoresis gel for the preparation of the polyclonal antibody.A rabbit was immunized with△MEG1 purified by preparative electrophoresis to generate anti-△MEG1 antibody.Anti-serum was collected on 28th day after initial injection and purified via affinity chromatography.The purified polyconal antibody displayed a satisfactory titer and sensitivity.     

茜素红S-铕(Ⅲ)-米诺环素体系的共振瑞利散射光谱研究及应用

在pH=4.4的NaAc-HAc缓冲溶液中,茜素红S-铕(Ⅲ)与米诺环素形成三元配合物,导致共振瑞利散射(RRS)增强,据此建立了一种测定米诺环素的RRS分析法。在λ=370nm处,米诺环素浓度在0.073～5.493μg/mL范围内与体系RRS强度呈良好的线性关系,检出限为0.021μg/mL。该方法灵敏度高,简单、快速,具有良好的选择性和重复性,对尿样进行加标回收及用于片剂、胶囊中米诺环素的测定,结果满意。     

GROUP-VELOCITY DISPERSION IN CHIRPED-PULSE AMPLIFIER

By using the pulse propagation equation in the nonlinear dispersive medium, the effects of second- and third-order group-velocity dispersion on pulse evolution in the stretching, amplification and compressing are studied when chirped pulse am-plification is applied to the pulses of a width <30fs, and the optimum dispersion-compensated conditions among the stretcher, amplifier and compressor are deter-mined. From the standpoint of dispersion compensation and control and the com-pressor adjustment, it is proposed that low-groove-density gratings (≤800 line/mm) are the better choices for stretching and compressing the pulses of a width <30fs in chirped-pulse amplification. It is also shown that, with a proper adjustment of the compressor incident angle and grating separation, an exact null in both second- and third-order dispersions can be achieved without any additional dispersion- compensated elements.     

不同类型石油卟啉标志物的光谱、质谱及色谱特征

本文利用紫外－可见光谱、质谱及色谱对ETIO、DPEP及Rhodo型石油卟啉标志物的特征性和相关性进行了研究，结果指出，在可见光谱带四个特征峰的吸收强度ETIO型为Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，DPEP型为Ⅳ＞Ⅱ＞Ⅲ＞ⅠRhodo型为Ⅲ＞Ⅱ＞Ⅳ＞Ⅰ，在反相高效液相色谱的体系中流出顺序为，先流出的是Rhodo型卟啉，其次是ETIO和DPEP型。不同类型卟啉的质谱特征是，各卟啉化合物基本以分子离子峰出现，极少碎片峰，同类卟啉化合物按正态分布。     

啁啾脉冲激光放大中的自相位调制效应

利用光与物质相互作用的半经典理论建立了啁啾脉冲激光放大的输运方程,数值分析了多程放大过程中自相位调制对啁啾脉冲时域特性和频域特性的影响研究结果表明,由于自相位调制引起附加频率啁啾产生,啁啾脉冲瞬时频率发生变化,放大特性受到影响.通过对增益饱和状态下自相位调制效应的研究,提出利用入射脉冲的线性频率啁啾抑制自相位调制的影响. 关键词：     

晶体压杆式压力传感器的动力传输性质

一般晶体压杆传感器的测时间隔均受压杆长度的限制。为此,我们研究了压杆的动力学性质。结果表明,要获得正确的压力波形,应设法减弱存在于压杆顶端部的径向振动和压杆尾端反射波对入射压力脉冲的干扰。本文提出波速色散压杆的设计,从而使传感器的性能改善,其最大优点在于测时间隔不受杆长的限制。     

First-Principles Study of Electronic Properties in PbS（1^-00） with Vacancy Defect

Electronic properties of both Pb and S vacancy defects in PbS（1^-00） have been studied using the first-principles density functional theory （DFT） calculations with the plane-wave pseudopotentials. It is found that the density of states （DOS） near the top of the valence band and the bottom of the conduction band is significantly modified by these defects. Our calculation indicates that in the case of S vacancy defects the Fermi energy shifts to the conduction band making it as an n-type PbS （donor）. However, in the case of Pb vacancy, because of the appreciable change of the DOS, the system acts as a p-type PbS （accepter）. In addition, the structural relaxation shows that the defect leads to outward relaxation of the nearest-neighbouring atoms and inward relaxation of the next-nearest neighbouring atoms.     

Growth of Semi-Insulating GaN Using N2 as Nucleation Layer Carrier Gas Combining with an Optimized Annealing Time

Semi-insulating （SI） GaN is grown using N2 as the nucleation layer （NL） carrier gas combined with an optimized annealing time by metalorganic chemical vapour deposition. Influence of using 1-12 and N2 as the NL carrier gas is investigated in our experiment. It is found that the sheet resistance of unintentionally doped GaN can be increased from 10^4 Ω/sq to 10^10 Ω/sq by changing the NL carrier gas from 1-12 to N2 while keeping the other growth parameters to be constant, however crystal quality and roughness of the tilm are degraded unambiguously. This situation can be improved by optimizing the NL annealing time. The high resistance of GaN grown on NL using N2 as the carrier gas is due to higher density of threading dislocations caused by the higher density of nucleation islands and small statistic diameter grain compared to the one using 1-12 as carrier gas. Annealing the NL for an optimized annealing time can decrease the density of threading dislocation and improve the tilm roughness and interface of AlGaN/GaN without degrading the sheet resistance of as-grown GaN signiticantly. High-quality SI GaN is grown after optimizing the annealing time, and AlGaN/GaN high electron mobility transistors are also prepared.     

可靠性物理动力学

本文试图从结构元件破裂的微观机理出发,用随机过程的理论方法,建立结构可靠性的微观动力学理论。文中给出了故障演化动力学方程和可靠性分析的基本几率函数,导出了结构元件的故障几率和几率密度、可靠性、故障率及平均寿命。 关键词：     

δ~2—加速的Broyden计算格式

应用 δ2 —加速技巧 ,对 Broyden的计算格式作了修正 .提出了一种新的修正的计算格式 ,给出了计算的步骤及数值例子 .