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Capillary electrophoretic analysis of alkaline phosphatase inhibition by theophylline 总被引:1,自引:0,他引:1
An analytical method for studying enzyme inhibition has been developed using capillary electrophoresis with laser-induced fluorescence detection. This technique is based on electrophoretic mixing of zones of enzyme and inhibitor in substrate-filled capillaries. Enzyme catalytic activity is measured by detecting the fluorescent reaction product as it migrates past the detector. Reversible enzyme inhibition is indicated by a transient decrease in product formation. The enzyme, alkaline phosphatase, has been studied using the fluorogenic substrate AttoPhos ([2,2'-bibenzothiazol]-6-hydroxy-benzthiazole phosphate). This assay has been used to quantify theophylline, a noncompetitive, reversible inhibitor of alkaline phosphatase. The detection limit for theophylline is estimated at 3 microM, and 8.6 amole of alkaline phosphatase are required for each assay. The calculated K(i) for theophylline is 90 microM for the capillary electrophoretic enzyme-inhibitor assays. 相似文献
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Accurate calculations of the dispersion dipole coefficient D 7 and its components are described for the systems HeH and HeHe, and used to check an approximate formula for D 7 developed in a previous paper [1]. By means of a further approximation for a new atomic property, values of D 7 are obtained for the rare-gas diatoms, based on semi-empirical Van der Waals C 6 coefficients, Hartree-Fock expectation values and sum-rule values for the atoms. The dispersion contributions to the dipole are compared with the Hartree-Fock contributions [35], and at the conventional collision diameters are found to be of comparable magnitude but usually of opposite sign. 相似文献
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