Investigation of the Doughnut Effect in Cy3-Labeled Protein Microarrays using Scanning Near-Field Optical Microscope

We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known “doughnut effect” observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image.     

金属氧化物薄膜的多离子束反应共溅射模型(Ⅱ)——数值计算与结果讨论

结合实验中的工艺技术参数,以Pb,Ti两金属靶的反应共溅射为例,对我们提出的金属氧化物薄膜的多离子束反应共溅射模型进行数值计算,分别得出了各靶的溅射速率R,反应腔中反应气体分压p以及衬底Pb,Ti的金属单质和氧化物所占的有效面积百分比与反应共溅射中直接可调的物理量,即反应气体总量Q和溅射离子束流J的关系.计算结果表明,该模型揭示了反应溅射具有滞回效应的本质特征,反映了反应共溅射中相关参数的相互影响与相互耦合的特点,给出了薄膜中组分原子百分比及其氧化物的形态与溅射工艺的关系,指出了多离子束反应共溅射中稳恒溅 关键词：     

Bioisosterism between Sulfonyl Group and Phosphoryl Group-The Synthesis of New ALS Inhibitors N-(Arylamino Hydroxyl Phosphoryl)-N'-(4,6-Dimethoxypyrimidine-2-yl)ureas

Sulfonylureaherbicidesisanewclassofherbicidesdiscoveredinthemid-l97O's',whosemodeofactionhasbeenestablishedastheinhibitionofacetolactatesynthase(ALS,alsoreferredasacetohydroxyacidsynthase)'-Muchattentionhasbeenfocussedonthiskindofcompound,sincetheyhaveunprecedenteduserates,ahighdegreeofselectivity,andexcellentenvironmentalsafety'.NumerousmodificationsofthestrUctureofthisclassofherbicideshavebeenreported'.Wehavetakenattentiontothesimi1alltybetweenthesulfonylgroup(-SO2-)andphosphoryIgroup[-P…     

Synthesis and Structural Characterization of Adduct [La(NO_3)_3·4H_2O]·2(BQEB)

Differentkindsofopenchaincrownethershavebeensynthesizedasthemodelcompoundsofionophoresandtheirinteractionswithsodium,potassiumandrareearthionshavealsobeenstudiedl'z.PanshowedCe(III)yieldedalf1complexwithl,8-bis(8'-quinolyloxy)-3,6-dioxaandtheCe(IIl)ioncoordinatedbothtoetheroxygenandquinolinenitrogenatoms3.TUmInleridentifiedtwolanthanidenitratecomplexeshavingastoichiometryof3:2(3RE:2L)'.InordertostUdythefunctionsofbothquinolineandbenzenering,introducedintothepolyetherchain,a1inearpolyether…     

韧性金属裂纹尖端损伤区内应变场测量——数字散斑相关法应用

本文用数字散斑相关方法测量了五种不同幂硬化指数韧性金属材料(铝和铜),双边裂纹尖端细观区域内应变场.对所得结果用韧性损伤模型进行了分析.在此法中以金属自然表面结构为散斑场,不同加载状态的散斑场进行比较,得到相对变形与应变.图象之间相关性 C 是变形参数或是位移及其导数的泛函.使其相关性 C 取最大值的试凑变形即为其真实变形场.这一方法在细观测量中应用得到满意的结果.     

Essay on gravitation: Present-time variation of Newton's gravitational constant in superstring theories

Superstring theories provide an appropriate framework for studying the time variation of fundamental coupling constants. The present time variation of coupling constants in Superstring theories with currently favorable internal backgrounds critically depends on the shape of the potential for the size of internal space. If the potential is almost flat, as in perturbation theory to all orders, the present value of ¦/G¦ for Newton's gravitational constant is calculable and estimated to be 1×10^–11±1yr^–1, which is just at the edge of the present observational bound for/G. If the potential has a minimum with finite curvature due to unknown nonperturbative effects,/G will become unobservably small. The improvement of the measurement of/G of 1 or 2 orders of magnitude would discriminate between the two situations. Problems with the time variation of other coupling constants are also discussed.This essay received the fourth award from the Gravity Research Foundation for the year 1987-Ed.     

水溶性席夫碱型锰配合物的合成、表征及其模拟超氧化物歧化酶活性研究

以水杨醛为母体, 与胺类化合物缩合形成席夫碱配体, 用分子自组装法合成了一系列水溶性席夫碱型金属锰单核、双核配合物. 通过元素分析、红外光谱对配合物进行了表征, 采用邻苯三酚自氧化法测定了配合物的超氧化物歧化酶(SOD)活性. 结果表明, 这些水溶性锰配合物具有良好的SOD活性.     

Re_2(edt)_4的电子结构和电子吸收光谱的从头算研究

用密度泛函理论中的B3LYP/CEP-4G方法对Re2（edt）4进行了结构优化和自然键轨道计算,并用单电子激发组态相互作用方法（CIS）计算了Re2（edt）4的分子轨道和激发态。结果表明Re-Re之间不存在明显的三重键,进而很好地解释了Re2（edt）4分子的电子吸收光谱。     

The Electrochemical Investigation of MEH-PPV in Ionic Liquid

Ionic liquids are a new kind of room-temperature molten organic salts. It can be used as solvents in the organic/polymeric syntheses, and some of them are called "green" solvents because of its recyclability1. Ionic liquids possess the superior properties, such as thermal stability up to 200(C, involatility and re-designability(for both cation and anion). The ionic liquid employed in this work is [bmim] [PF6]- ( butylmethylimidazolium hexafluorophosphate ) which is immiscible with water and h…     

麻黄中伪麻黄碱在小鼠组织中的分布研究

为了解麻黄(Herba ephedrae,简称HE)中伪麻黄碱(Pseudoephedrine,简称PE)在小鼠脑、肺、心、肝、肾的分布变化,探讨HE的归经,用GC-MS/SIM法,分析了用药后30、60、120 min PE在小鼠脏器分布量。结果表明,PE于不同时间点在小鼠脏器的分布由多到少的顺序是:30 min时,肺脏(肾脏、肝脏、脑),心脏;60 min时,肾脏,心脏(肝脏),脑,肺脏;120 min时,脑,肾脏,心脏,肝脏(肺脏)。本试验从PE在小鼠体内脏器的分布量初步反映了HE的归经趋势。