不同构象铂(Ⅱ)配合物与G-肌动蛋白作用的光谱研究

The cyclohexamethylene-diamine squaric acid platinum(Ⅱ) complexs(R,R, S,S) binding to G-actin and the consquence conformation change were studied by N-(1-pyrenyl) maleimide(NPM) labeled fluorescence and intrinsic fluorescence. The binding and chemical kinetics were determined by fluorometry. The results showed that when R (Pt/actin molar ratio) was lower than 30, the Pt binded to G-actin strong binding sites(-SH), when R was larger than 30, the Pt binded to G-actin weaker binding sites. The consquence conformation changes were determined by CD. The CD results showed that R was smaller than 60, G-actin α-helix contents decreased slowly, R was larger than 60, G-actin conformation changed obviously. The reaction of R,R complex of platinum(Ⅱ) S,S complex of platinum(Ⅱ) with G-actin was similar to that of with S,S complex of platinum(Ⅱ).     

Dynamics of excited m-dichlorobenzene

Dichlorobenzene is a precursor molecule of di-oxins, which are some of the most toxic chemicals and are suspected of being mutagenic with the structure of benzene rings containing different numbers of chlorine atoms. They are seriously harmful to the health of human. Therefore, it is necessary to study these aryl chemicals especially dichlorobenzene molecules in great detail. In recent years, a number of groups have investi-gated the molecules of aryl halide. The near ultraviolet absorption sp…     

分散法制备的CuCl/MCM-41上C3H6选择催化还原NO反应的研究

研究了由分散法制备的两种CuCl/MCM-41催化剂上丙烯在过量氧存在下选择催化还原NO反应, 发现所制备的CuCl/AlMCM-41催化剂的反应活性明显高于CuCl/SiMCM-41. XRD, IR, TPR及ESR的研究结果表明, CuCl/AlMCM-41催化剂上的主要活性中心是与骨架铝配位的铜离子(Cu2+/Cu+).     

pH值和钙离子对尖吻蝮蛇抗血小板凝集素二级结构的影响

尖吻蝮蛇毒中抗血小板凝集素是凝血因子IX/凝血因子X结合蛋白，它具有抗凝血和抑制血小板凝集双重活性。用红外光谱、拉曼光谱和CD谱研究了抗血小板凝集素的二级结构以及pH值和钙离子对其二级结构的影响。用CD谱测得，在水溶液中，抗血小板凝集素的主要骨架构象为β-折叠(26.3%)和α-螺旋(19.6%)结构。拉曼光谱显示，在粉末状态，其α-螺旋含量显著降低。CD谱还表明，抗血小板凝集素在pH值3.0～11.0范围内保持稳定的天然结构，钙离子诱导的抗血小板凝集素结构变化是可逆的，钙离子在稳定抗血小板凝集素的天然结构中起重要作用。     

基于Gene Ontology的表达谱与基因功能相似性的相关性研究

聚类是芯片数据分析中被广泛使用的方法。未知基因的功能通常通过其与已知基因在不同生物状态下具有表达相似性来进行预测。然而，还未有人就这种通过表达相似性来进行功能注释的方法的可靠性进行评估。本文利用Gene Ontology对表达相似性和基因功能相似性的相关关系进行了全面的研究。研究表明，尽管表达谱的相似性和基因功能相似性之间有一定的依赖关系，但相关性较弱。在Gene Ontology的三大类中，相对生物过程和分子功能，基因表达谱的相似性更有助于细胞组分的注释。本文的研究结果对于基因功能的预测有一定的指导意义。     

藏药麻花艽中四种苦苷类化学成分的HPLC测定

建立了藏药麻花艽中龙胆苦苷(gentiopicroside)、落干酸(loganic acid)、獐牙菜苦苷(swertiamarin)和獐牙菜苷(sweroside)4种苦苷类成分的HPLC分析方法。色谱柱为Eclipse XDB-C8(4.6 mm i.d.×150 mm,5μm),流动相为体积分数5%乙腈 10 mmoL甲酸~95%乙腈水溶液,检测波长为240 nm。结果表明,该方法具有良好的线性关系和回收率,为麻花艽中苦苷类成分的进一步开发利用提供了科学依据。     

Sonogashira反应研究的最新进展

对Sonogashira反应的最新研究进展作了综述. 详细地介绍了Sonogashira 反应的最新优化、联串化、绿色化以及非Pd催化的Sonogashira反应等, 同时还综述了Sonogashira反应的机理研究.     

金属络合物的相转移催化分析与分离

以可简单分离回收、可反复使用为目标，详细介绍了相转移催化的变化发展过 程．通过对溶剂的选择，使得催化剂选择性地溶解在某一液相中，而使产物溶解在 另一液相中，如水-有机两相催化体系；通过温度的变化，简单地实现了在较高温 度下反应为均相体系以提高催化剂的活性，而在较低温度下实现了催化剂与产物不 相溶使得催化剂得以简单分离，如温控型水-有机两相催化体系、氟-有机两相催化 体系、温控型含氟催化剂、温控型有机金属催化剂等．     

Hydrothermal Synthesis and Crystal Structure of [（CH3CH2）4N]4[（La12（OH）12MoO29）],A Novel Cluster Compound with a MoO5 Hexahedron Center surrounded by La—O Octahedron

IntroductionRecently,the molecular designs of polyox-ometalates( POM) with basic oxometal clusterbuilding blocks are of great interest[1,2 ] . Hy-drothermal synthesis,with the characteristic ofone- pot reaction,provides a convenient method toobtain novel structures of POM compounds[3— 6] . Inthe Ln/Mo/O system ( Ln=rare earth) ,somecompounds containing La and Mo elements havebeen reported[7— 17] . However,these compoundshave all been synthesized under the highly restrict-ed synthetic co…