Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA

A solvent tolerant Pseudomonas aeruginosa PseA strain was isolated from soil. It secreted a novel alkaline protease, which was stable and active in the presence of range of organic solvents, thus potentially useful for catalysis in non-aqueous media. The protease was purified 11.6-fold with 60% recovery by combination of ion exchange and hydrophobic interaction chromatography using Q-Sepharose and Phenyl Sepharose 6 Fast Flow matrix, respectively. The apparent molecular mass based on the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was estimated to be 35,000 Da. The enzyme was stable in the pH range of 6.0-9.0, the optimum being 8.0. The Km and Vmax towards caseinolytic activity were found to be 2.7 mg/ml and 3 micromol/min, respectively. The protease was most active at 60 degrees C and characterized as a metalloprotease because of its sensitivity to EDTA and 1,10-phenanthroline. It was tested positive for elastase activity towards elastin-orcein, thus appears to be an elastase, which is known as pseudolysin in other strains of P. aeruginosa. The protease withstands range of detergents, surfactants and solvents. It is stable and active in all the solvents having log P above 3.2, at least up to 72 h. These two properties make it an ideal choice for applications in detergent formulations and enzymatic peptide synthesis.     

N-halogeno-compounds. Part 9 [1]. Azoxy- and azo-arenes derived from 4-(dichloroamino)tetrafluoropyridine; crystal structure of trans-2,3,5,6-tetrafluoro-4-(2,4,6-trimethylphenyl-onn-azoxy)pyridine

The nitrosoarenes ArNO (Ar = C₆H₅, 2-MeC₆H₄, 2,4,6- Me₃C₆H₂ and C₆F₅) have been condensed with 4-(dichloroamino)- tetrafluoropyridine to provide the azoxy-compounds py_FN$\text{N}\text{+}$($\text{N}\text{-}$)Ar (py_F = 2,3,5,6-tetrafluoro-4-pyridyl); de-oxygenation of the first three with triphenylphosphine or triethyl phosphite gave the corresponding azo-compounds, and the reverse reaction was achieved in the case of py_FNNC₆H₂Me₃-2,4,6 using peroxytrifluoroacetic acid. Thermolysis of 4-azidotetrafluoropyridine in the presence of pentafluoronitrosobenzene provided the perfluorinated azoxy-compound py_FN$\text{N}\text{+}$($\text{O}\text{-}$)C₆F₅. X-Ray methods have been used to determine the molecular geometry of py_FN$\text{N}\text{+}$($\text{O}\text{-}$)C₆H₂Me₃-2,4,6.     

Novel perturbation expansion for the Langevin equation

We discuss the randomly driven systemdx/dt= -W(x) +f(t), wheref(t) is a Gaussian random function or stirring force withf(t)f(t)=2(t–t), andW(x) is of the formgx ¹⁺². The parameter is a measure of the nonlinearity of the equation. We show how to obtain the correlation functionsx(t)f(t)···x(t( n)) _f as a power series in. We obtain three terms in the expansion and show how to use Padé approximants to analytically continue the answer in the variable. By using scaling relations, we show how to get a uniform approximation to the equal-time correlation functions valid for allg and.     

Determining theCP-violating phase γ

The weak phase γ is conventionally probed by theB _s→ρ ⁰ mode. The predicted rate is tiny. Even if aB _s→ρ ⁰ K _s rate difference could be established, it would not be clear that sin 2γ had been measured, because amplitudes with other weak phases may contribute significantly. Non-CP eigenstates, such asB _s →D _s ^± K ^?, have a two-fold advantage overB _s→ρ ⁰ K _s. Their rates are orders of magnitude above that forB _s→ρ ⁰ K _s, and they probe theCP-violating phase γ, without any contamination from other weak phases. Detailed time-dependent studies of non-CP eigenstates remove possible final-state phases and extract the weak phase γ.     

Evaluating the Frechet derivative of the matrix exponential

LetM _n denote the space ofn×n matrices. GivenX, ZM _n define

Energy dependence of the fast fragment of the target nucleus in high energy hadron-nucleus interaction

This paper presents an extensive study on the dependence of the mean number of the fast fragment of the target nucleus n _g on the incident beam energy in proton-nucleus interaction in emulsion in the range 6·2E ₀400 GeV/c. It has been observed that n _g decreases in the range 6·2E ₀200 GeV/c, then increases and attains an approximately steady value up to 400 GeV/c. It is very difficult to explain this behaviour with the help of the existing nuclear production models.The author would like to thank Prof A. J. Herz (CERN), Prof. K. D. Tolostov (Dubna, U.S.S.R.), Prof. P. L. Jain (State University of New York, U.S.A.), Prof. G. Giacomelly (Italy) for kindly supplying the exposed emulsion plates.     

THE EFFECTS OF FAR ULTRAVIOLET RADIATION ON RESPIRATION AND CATION ACCUMULATION BY ISOLATED MITOCHONDRIA OF PHASEOLUS VULGARIS

Abstract. The respiration rates and respiratory control ratios of isolated bean mitochondria have been measured following exposure to 0, 150, 300 and 900 J/m² of far UV radiation (190–300 nm) from a mercury vapour light source with 90% total radiant intensity at 254 nm. Loss of respiratory control occurred at 150 J/m² and inhibition of respiration was significant at the highest exposure dosage. The uptake of both ⁴⁵Ca and ⁸⁵Sr have been measured following a 10min incubation of isolated mitochondria with 2 m M cation. Significant decreases in cation accumulation were observed following exposure to 900 J/m². The effect seemed to be associated with loss of active transport of the ions as a result of respiratory uncoupling or reduced electron transport. There was no significant effect of storage on respiration or ion transport nor was there any indirect effect of irradiated suspending medium on mitochondria.