Boronic Acid-Mediated Activity Control of Split 10–23 DNAzymes

The 10–23 DNAzyme is an artificially developed Mg²⁺-dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence-specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg²⁺ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best-performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.     

Differential protein expression by Pseudomonas fragi submitted to various stresses.

We have analyzed the impact of various stressing conditions on the physiological and molecular responses of the main psychrotrophic spoilage bacterium of refrigerated meat and meat products, Pseudomonas fragi. A survival study using conventional plating was first performed to select the stressing agents and parameters. Some of these mimicked cleaning-disinfection processes but with less drastic conditions in order to keep alive enough bacterial cells for the protein expression characterization. Cultures of P. fragi, at the beginning of the stationary phase of growth, were submitted to individual pH (5.4, 10.5), osmotic (8% Na2SO4, pH 7.0), biocide (fatty amine) shocks or combined treatments (8% Na2SO4, pH 10.5; 8% Na2SO4, pH 10.5 + biocide; pH 5.4 + pH 10.5 and pH 10.5 + pH 5.4) and the molecular responses were investigated by comparing autoradiograms of two-dimensional gel electrophoresis (2-DE) patterns of proteins radiolabeled with L-[35S]methionine. The observation of qualitative and relative quantitative variations in protein expression, determined with Melanie II image analysis software (Bio-Rad), revealed the overexpression of a total of 91 proteins for the eight challenges by comparison with the nonshocked controls. Some proteins appeared to be more or less general stress proteins whereas others were specific for one chemical treatment. The appraisal of the type of molecular response according to the type of treatment was analyzed statistically.     

Giant stop bands and defect modes in one-dimensional waveguide with dangling side branches

We investigate both the photonic and electronic band structure of a comb-like waveguide geometry in which dangling side branches are grafted along an infinite one-dimensional waveguide. In a periodic (superlattice like) waveguide, we report the opening-up of stop bands which originate both from the periodicity of the system and the resonant states of the grafted branches (which play the role of resonators). Wide gaps (narrow bands) can be obtained by grafting several dangling side branches at every node. The stop bands still remain even for identical constituent materials. We also propose a tandem structure composed of two or several successive combs which differ by their physical characteristics that allows an ultrawideband filter. This behavior results from the superposition of the bandgaps in the successive structures. The presence of a defect branch in the comb can give rise to localized modes inside gaps. These states appear as very narrow peaks in the transmission spectrum and therefore may have useful applications in the frame of photonic bandgap materials or electronic band engineering of nanostructures.     

Synthesis of Homo- and Heterofunctionalized Glycoclusters and Binding to Pseudomonas aeruginosa Lectins PA-IL and PA-IIL

Homo- and heterofunctionalized glycoclusters with galactose and/or fucose residues targeting both PA-IL and PA-IIL lectins of Pseudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry. Their binding to lectins (separately or in a mixture) was studied using a DNA Directed Immobilization carbohydrate microarray. Homoglycoclusters bind selectively to their lectin while the heteroglycocluster binds simultaneously both lectins with a slight lower affinity.     

New strategies for cyclization and bicyclization of oligonucleotides by click chemistry assisted by microwaves

The synthesis of cyclic, branched, and bicyclic oligonucleotides was performed by copper-catalyzed azide-alkyne cycloaddition assisted by microwaves in solution and on solid support. For that purpose, new phosphoramidite building blocks and new solid supports were designed to introduce alkyne and bromo functions into the same oligonucleotide by solid-phase synthesis on a DNA synthesizer. The bromine atom was then substituted by sodium azide to yield azide oligonucleotides. Cyclizations were found to be more efficient in solution than on solid support. This method allowed the efficient preparation of cyclic (6- to 20-mers), branched (with one or two dangling sequences), and bicyclic (2 x 10-mers) oligonucleotides.     

Strontium ferrite nanoparticles synthesized in presence of polyvinylalcohol: Phase composition,microstructural and magnetic properties

Systematic study was devoted to the synthesis of hexagonal strontium ferrite nanoparticles employing polyvinylalcohol as stabilizing agent. Preliminary experiments allowed to select an optimal sol having molar ratio Sr²⁺/Fe³⁺=12${\mathrm{Sr}}^{2+}/{\mathrm{Fe}}^{3+}=12$, weight ratio PVA/[Sr²⁺+Fe³⁺]=1.4$\mathrm{PVA}/[{\mathrm{Sr}}^{2+}+{\mathrm{Fe}}^{3+}]=1.4$ and pH=2.1$\mathrm{pH}=2.1$. The obtained sol were transformed to gels by an evaporation of water at 100 °C and drying at 112 °C under vacuum. The subsequent calcination was carried out for 3 h at 400 °C, achieved by heating rate of 17 K/min. The obtained precursor was used for a detail study of influence of annealing conditions (temperature range 600–700 °C, annealing time 10–190 min) on the resulting properties. Semiquantitative X-ray phase analysis approved a gradual increase of the M-phase content and a gradual growth of M-phase crystallites with temperature and time. Magnetic measurements showed a distinct influence of the phase composition, namely ratio of the contents of M-phase and maghemite on the shape of the magnetic loops, while the crystallite sizes have only a slight effect.     

Global Regularity of Solutions to Systems of Reaction–Diffusion with Sub-Quadratic Growth in Any Dimension

This paper is devoted to the study of the regularity of solutions to some systems of reaction–diffusion equations, with reaction terms having a subquadratic growth. We show the global boundedness and regularity of solutions, without smallness assumptions, in any dimension N. The proof is based on blow-up techniques. The natural entropy of the system plays a crucial role in the analysis. It allows us to use of De Giorgi type methods introduced for elliptic regularity with rough coefficients. In spite these systems are entropy supercritical, it is possible to control the hypothetical blow-ups, in the critical scaling, via a very weak norm. Analogies with the Navier–Stokes equation are briefly discussed in the introduction.     

Puzzle of bulk conformal field theories at central charge c = 0

Nontrivial critical models in 2D with a central charge c=0 are described by logarithmic conformal field theories (LCFTs), and exhibit, in particular, mixing of the stress-energy tensor with a "logarithmic" partner under a conformal transformation. This mixing is quantified by a parameter (usually denoted b), introduced in Gurarie [Nucl. Phys. B546, 765 (1999)]. The value of b has been determined over the last few years for the boundary versions of these models: b(perco)=-5/8 for percolation and b(poly)=5/6 for dilute polymers. Meanwhile, the existence and value of b for the bulk theory has remained an open problem. Using lattice regularization techniques we provide here an "experimental study" of this question. We show that, while the chiral stress tensor has indeed a single logarithmic partner in the chiral sector of the theory, the value of b is not the expected one; instead, b=-5 for both theories. We suggest a theoretical explanation of this result using operator product expansions and Coulomb gas arguments, and discuss the physical consequences on correlation functions. Our results imply that the relation between bulk LCFTs of physical interest and their boundary counterparts is considerably more involved than in the nonlogarithmic case.