Synthesis of morpholine nucleoside triphosphates

Triphosphates of all four ribonucleoside derived morpholine subunits were synthesized and characterized by ¹H and ³¹P NMR, UV and mass spectroscopy.     

The role of a reference sample in the study of the titanium-containing silicas by ultraviolet-visible diffuse reflectance spectroscopy

On the example of the silica gel with grown titanium oxide structures, in which titanium has two coordination enviromments (anatase, rutile) we showed the effect of the choice of a reference (MgO, SiO₂) on the shape of the ultraviolet-visible diffuse reflectance spectra and the result of their mathematical processing (the Kubelka-Munk function, first derivative).     

Interaction of titanium tetrachloride vapors with zirconium dioxide nanocrystals

Products of interaction of the surface of zirconium dioxide nanocrystals with titanium tetrachloride as a test reagent were studied. The composition and structure of the surface of synthesized products depending on the time of treating the substrate by titanium chloride vapor were determined by a complex monitoring of both chemical conversions in the gas phase and concentrations of components in the solid-phase matrix with the help of gravimetric “in situ” measurements at various stages of the reaction.     

Mosaic microinhomogeneity in crystals of (Co,Ni)K2(SO4)2 · 6H2O solid solutions

The mosaic microinhomogeneity in mixed crystals of the isomorphic series (Co,Ni)K₂(SO₄)₂ · 6H₂O has been studied. The growth processes have been observed in situ in optical and confocal microscopes and the distribution of composition components has been studied by the energy-dispersive method. The results make it possible to develop model concepts about the formation of composition microinhomogeneity in solid solutions.     

Synthesis of functionalized α-CF3-α-aminophosphonates via Cu(I)-catalyzed 1,3-dipolar cycloaddition

A convenient method for the preparation of α-CF₃-α-aminophosphonates bearing alkynyl group at the α-carbon atom has been described. New alkynylphosphonates have been further utilized in the synthesis of functionalized triazole-containing α-CF₃-α-aminophosphonates via copper-catalyzed (3+2)-cycloaddition to different organic azides.     

Synthesis of Gold Glyconanoparticles Based on Thiol-Containing d-Hexose Acylhydrazones and Their Modification by Thiolated Poly(2-deoxy-2-methacryloylamino-D-glucose)

Russian Journal of General Chemistry - A procedure has been developed for the synthesis of gold glyconanoparticles with an average particle size of 15–30 nm and a low polydispersity index on...     

The Paths of Excitation Energy Deactivation in LH1 Reduced Mutant and Wild-Type Strains of Rhodobacter sphaeroides

The P3 mutant of Rhodobacter sphaeroides had an altered ratio of reaction center to core (LH1) and peripheral (LH2) antenna complexes compared to the wild-type strain. Intracytoplasmic membranes from these two strains were purified and then resuspended in buffer or immobilized in isotropic and stretched polymer film. The absorption, photoacoustic, and delayed luminescence spectra were measured. The ratios of infrared absorption and photoacoustic bands (located at about 880 nm for LH1 and at 850 and about 800 nm for LH2) as well as the half-width of these bands are different for the LH2 and LH1 mutants and wild-type strain. The whole yields of thermal deactivation of the two strains were comparable, but in the absorption region of LH2 it was slightly lower in the case of the mutant than for the wild-type strain. The delayed luminescence main maxima were observed at about 860 and 700 nm. The first one could be due to emission of bacteriochlorophyll a of LH2 complexes. The emission at about 700 nm is probably due to dihydromesochlorophyll, which is usually, to some extent, produced from bacteriochlorophyll a in bacterial complexes. The delayed luminescence emission is competing with excitation energy transfer to the reaction center. The intensity of the delayed luminescence of the mutant strain was higher than that of the wild-type strain when both samples were excited in a region of carotenoid absorption. The mutant contains less carotenoids than the wild-type strain. Carotenoids work as efficient antenna. When they at a lower concentration the excitation can be trapped more easily by some chlorophyll-like pigment isolated from the excitation energy chain. The dependences of delayed luminescence spectra on the light polarization and excitation wavelengths for the wild-type strain and for the mutant were different. The anisotropy of delayed luminescence showed that bacteriochlorophyll a molecules of different orientations were contributing to the mutant and the wild-type strain emission. All the results suggest that the excitation energy transfer from the antenna to the reaction center in the mutant and the wild-type strain is similar.     

Development of a chip-based capillary gel electrophoresis method for quantification of a half-antibody in immunoglobulin G4 samples

A method based on microfluidic technology was developed to support quantitative analysis of recombinant monoclonal immunoglobulin G4 (IgG4) antibody samples. The assay was performed on an Agilent 2100 Bioanalyzer in combination with the Protein 200 Plus LabChip Kit and the Protein 200 Plus assay software. Capillary electrophoresis principles have been transferred to a chip format that integrates all separation, staining, virtual destaining, and detection steps. The method is referred to in this paper as chip-based capillary gel electrophoresis (GelChip-CE method). The GelChip-CE method under nonreducing conditions proved to be a quantitative test for half-antibody determination in IgG4 samples. Similar to the traditional nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, the GelChip-CE method includes a denaturing step prior to separation. We showed that denaturing the sample by heating resulted in an artificial increase in the amount of half-antibody detected, which could be prevented by addition of N-ethylmaleimide to the sample buffer. The GelChip-CE method allowed for analysis of IgG4 samples with more accuracy, higher precision, and a faster turnaround time than SDS-PAGE and reversed-phase high-performance liquid chromatography (RP-HPLC).