Activation of mouse macrophages by in vivo and in vitro treatment with a cyanine dye, lumin.

The cyanine photosensitizer, lumin, is a potent macrophage activating agent: 4 days after administration of small amounts of lumin to mice (20-40 ng mouse-1), peritoneal macrophages exhibited a greatly enhanced Fc-mediated ingestion activity; higher doses (more than 3000 ng mouse-1) did not have this effect. The in vitro photodynamic activation of macrophages in mouse peritoneal cells exposed to white fluorescent light (3 J m-2 s-1) was also studied in media containing various concentrations of lumin. A short light exposure (45 J m-2) with 10 ng lumin ml-1 produced a maximum ingestion activity of macrophages. Lumin has absorption peaks at 670 and 760 nm. Therefore we designed experiments in which peritoneal cells were exposed to a red fluorescent light (emission, 660 nm; 0.5 J m-2 s-1). In a medium containing 3 ng lumin ml-1 with 7.5 J m-2 of red light, a markedly enhanced ingestion activity of macrophages was observed. The photodynamic treatment of peritoneal macrophages alone did not stimulate phagocytic activity, but the photodynamic treatment of a mixture of non-adherent (B and T) cells and macrophages resulted in a greatly enhanced ingestion activity of macrophages. Thus non-adherent cells are required for the photodynamic activation of macrophages, implying that an activating factor is generated within the non-adherent cells and transmitted to the macrophages. This hypothesis was confirmed by the observation that co-cultivation of photodynamically treated non-adherent cells with untreated macrophages resulted in a greatly enhanced ingestion capacity.     

Coherent convergent beam electron diffraction for analysis of lattice fitting in PbTe/MgO bi-crystals

Coherent convergent beam electron diffraction is for the first time applied to the analysis of lattice fitting between vacuum-deposited PbTe and MgO thin crystals in the plan-view mode. The possibility of determination of the rigid-body shift is discussed on the basis of simulated diffraction patterns from the bi-crystals as well as the preliminary experimental results obtained by a 200 kV transmission electron microscope with a field emission electron gun. In the analysis, the importance of the position of interference fringes at overlaps of PbTe 200 and MgO 200 diffraction disks and that of the corresponding mirror symmetry in the whole pattern are emphasized in order to determine the rigid-body shift and the probe-position.     

Decisive factors for realizing atomic-column resolution using STEM and EELS

We demonstrate atomic-column imaging by scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy (EELS). The silicon atomic-columns of a β-Si₃N₄ (0 0 1) specimen are clearly resolved. The atomic-site dependence and the energy-loss dependence of the spatial resolution are elucidated on the basis of the experimental results and multislice calculations. We describe two decisive factors for realizing atomic-column imaging in terms of localization in elastic and inelastic scattering. One is the channeling of the incident probe due to dynamical diffraction, which has atomic-site dependence. The other is the localization in inelastic scattering; in addition to the energy-loss dependence of delocalization, we point out its dependence on the offset energy from the ionization energy, i.e., an additional localization factor concerning the Bethe surface. The present atomic-column observation of the Si-L core-loss image indicates that the local approximation, which can be interpreted intuitively, is achievable under appropriate experimental conditions, such as high-energy-loss, a small convergence angle and a large collection angle (e.g., 400 eV, 15 and 30 mrad, respectively).     

Charge/orbital ordering structure of Pr(1-x)Ca(x)MnO(3)(x = 3/8) examined by low-temperature transmission electron microscopy

The structural phase transition of Pr(1-x)Ca(x)MnO(3)(x = 3/8) was investigated by means of low-temperature transmission electron microscopy. Superlattice reflection spots with a modulation wave vector q(1) = (0,1/2,0) appeared below 230 K, indicating formation of the d(3x(2-r(2))/d(3y(2)-r(2)) type of charge/orbital ordering. Below 150 K, a new series of superlattice reflection spots with a modulation wave vector q(2) = (1/4,1/4,1/2) appeared, suggesting an additional ordering of excess 1/8 Mn(3+), necessary due to the deviation of x from 1/2, with the occupation of the d(3z(2-r(2)) type of e(g) orbital.     

The solvent extraction of europium and barium with 1-aryl-3-methyl-4-aroyl-5-pyrazolones

Eleven 1-aryl-3-methyl-4-aroyl-5-pyrazolone derivatives were synthesized. Their acid dissociation constants were determined spectrophotometrically; there was a linear relationship between them and Hammett's σ values. Europium and barium ions were readily extracted into benzene and n-octyl alcohol, respectively, at very low pH.     

Die Anwendung eines Mehrfach-Detektorsystems zur stereoskopischen Rasterbeobachtung in der Elektronenstrahl-Mikroanalyse

Zusammenfassung Nach einer Übersicht über die Informationsmöglichkeiten der Elektronenstrahlmikroanalyse wird eine neue Methode der stereoskopischen Detektion von der Probenoberfläche rückgestreuter Elektronen dargestellt. An Hand von Beispielen wird gezeigt, daß dadurch eine gute Unterscheidung zwischen Oberflächentopographie und chemischer Zusammensetzung der Gefügebestandteile an der Probenoberfläche möglich ist.

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Summary Following a review of the information possibilities of the electron beam microanalysis, a new method is presented involving the stereoscopic detection of the electrons sprayed back from the surface of the specimen. By means of examples it is shown that in this way it is possible to differentiate well between surface topography and chemical composition of the structural components on the surface of the specimen.

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Résumé Après une vue d'ensemble sur les possibilités d'informations microanalytiques par irradiation électronique, on présente une nouvelle méthode de détection stéréoscopique de la surface des échantillons par diffraction électronique en retour. On montre en se servant d'exemples qu'une bonne distinction entre la topographie superficielle et la composition chimique des constituants de la texture est ainsi possible sur la surface de l'échantillon

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Vortrag anläßlich des Kolloquiums für metallkundliche Analyse mit besonderer Berücksichtigung der Elektronenstrahl-Mikroanalyse, Wien, 22. Oktober 1964.     

Observation of magnetic ripple and nanowidth domains in a layered ferromagnet

We investigated ferromagnetic domain structures on nanometer to micrometer scale for single crystals of a layered ferromagnet, La(2-2x)Sr(1+2x)Mn2O7 (0.32 < or = x < or = 0.40), as functions of x and temperature by means of Lorentz electron microscopy. We have succeeded in observing the evolution of magnetic ripple structure, dynamically, related to a spin reorientation transition where the magnetization direction switches between parallel and perpendicular to the layers. Our high-resolution magnetic domain imaging revealed that the ripple state is characterized by the evolution of magnetic nanowidth domains.