Star-shaped polymers for DNA sequencing by capillary electrophoresis

The separation and sequencing of DNA are the main objectives of the Human Genome Project, and this project has also been very useful for gene analysis and disease diagnosis. Capillary electrophoresis (CE) is one of the most common techniques for the separation and analysis of DNA. DNA separations are usually achieved using capillary gel electrophoresis (CGE) mode, in which polymer gel is packed into the capillary. Compared with a traditional CGE matrix, a hydrophilic polymer matrix, which can be adsorb by the capillary wall has numerous advantages, including stability, reproducibility and ease of automation. Various water-soluble additives, such as linear poly(acrylamide) (PAA) and poly(N,N-dimethylacrylamide) (PDMA), have been employed as media. In this study, different star-shaped PDMA polymers were designed and synthesized to achieve lower polymer solution viscosity. DNA separations with these polymers avoid the disadvantages of high viscosity and long separation time while maintaining high resolution (10 bp between 271 bp and 281 bp). The influences of the polymer concentration and structure on DNA separation were also determined in this study; higher polymer concentration yielded better separation performance, and star-like polymers were superior to linear polymers. This work indicates that modification of the polymer structure is a potential strategy for optimizing DNA separation.     

Spectrofluorimetric determination of phenylalanine based on fluorescence enhancement of europium ion immobilized with sol-gel method

The analysis of phenylalanine (Phe) in serum is widely performed for the screening of newborn phenylketonuria (PKU). In this work, a novel spectrofluorimetric method for the determination of Phe was developed based on the fluorescence enhancement of Ruhemann's purple, the reaction product between Phe and ninhydrin, upon coordination with Eu(3+). A filter paper disc containing immobilized reactants (ninhydrin and Eu(3+)) was fabricated by sol-gel method. The experimental parameters affecting the determination of Phe, such as the concentrations of immobilized reagents, the pH value, the reaction time and temperature were optimized. Under optimum conditions, the fluorescence intensity of Phe-ninhydrin-Eu(3+) system was linearly proportional to the concentration of Phe in the range from 5×10(-5) to 2×10(-3) mol L(-1), and the limit of detection was found to be 5.2×10(-6) mol L(-1). The relative standard deviation was 2.6% for ten replicate measurements of 1.5×10(-4) mol L(-1) of Phe. The method has merits of sensitivity, simplicity and low cost, and has been applied to the determination of Phe in artificial serum.     

Electric-field induced elastic stretching of multiwalled carbon nanotube clusters: a realistic model

The oscillating electric-field induced stretching phenomenon of multiwalled carbon nanotube (MWCNT) clusters in liquid crystal medium demonstrates distinct threshold behaviour under optical microscopic investigation. The optimum field required for the initiation of MWCNT cluster stretching is found to depend on their length in the field-off state. The phenomenon has been explained in light of a classical theoretical model assuming MWCNT agglomerates as a single electric dipole. The spring constant and induced charge obtained by fitting the formulated theoretical model show good agreement with previous reports, hence establish the proposed dipolar reorientation mechanism of MWCNT clusters induced by the electric field.     

Three New Urea Derivatives from Pliocene‐Fossil Pinus armandii

Three new urea derivatives, isolated from the Pliocene lignified wood of Pinus armandii, were identified as carbonylbis[imino(6‐methyl‐3,1‐phenylene)]bis[carbamic acid] dimethyl ester ( 1 ), and as the corresponding dibutyl ester 2 and bis(2‐methylpropyl) ester 3 . Their structures were elucidated by spectroscopic methods, including MS and 1D‐ and 2D‐NMR techniques.     

用自适应MG法,FMG法求解Helmholtz方程

本文针对Ｖ循环、Ｗ循环和多重网格法中最优光滑次数及循环体个数难以确定的缺点，以Ｈｅｌｍｈｏｌｔｚ方程为例给出自适应的多重网格算法和自适应的完全多重网格算法。     

Chiral planar waveguide for guiding single-mode backward wave

Single-mode backward wave is shown to be guided in a planar dielectric waveguide with a strong chiral core. The significant difference of such a waveguide from the traditional one is the guidance of single-mode backward wave, without using negative permittivity and/or negative permeability. In the design, we generalize the idea of total internal reflection to the chiral medium and make a numerical analysis on the reflection with oblique incidence. We deduce rigorously a general solution of incident wave on the boundary of two arbitrary chiral magneto-electric media. We observe that the impedance matching can eliminate the coupling between two eigenwaves in chiral media. With strong chiral core and the matched impedance with cladding, one eigenwave becomes a backward wave and can be guided without transferring to the other eigenwave. If a single-mode propagation condition is satisfied, we will get single-mode backward guided wave. A special interface has been designed to prevent the forward wave entering the waveguide from the source.     

三元乙丙橡胶的13C-NMR定量分析

用13C核磁共振波谱法对三元乙丙橡胶进行了研究，通过图谱中各个峰的积分值，并用特定的计算公式测定了三元乙丙橡胶中乙烯及第三单体乙叉降冰片烯的含量，为红外光谱批量的定量分析建立了基准化的标定。     

有限元Ritz-Volterra投影的超收敛性质及其应用

本文研究有限元Ritz－Volterra投影的超收敛性质．利用一种新型的Green函数，证明了该投影具有与有限元Ritz投影相平行的函数和导数逼近的超收敛性质．这些结果被应用于抛物型积分微分方程和Sobolev方程的半离散有限元近似．     

Two novel molybdenum complexes containing [Mo2O2S2]2+ fragment: synthesis,crystal structures and catalytic studies

Mo₂O₂S₂(HGly)(Gly)₂ 1 and K₆[Mo₂O₂S₂(nta)₂][Mo₂O₂S₂(ntaH)₂]^·4H₂O 2 were synthesized by the reactions of (NH₄)₂MoS₄ and amino acids L (L = glycine, nitrilotriacetic acid) in ethanol–water medium at ambient temperature. The two complexes were characterized by elemental analysis, infrared spectra, UV–visible spectra, TG–DTA and XPS. X‐ray crystallographic structural analyses revealed that compound 1 is a binuclear Mo? S? glycinate complex, a glycinate ligand is coordinated to each molybdenum atom through its amine nitrogen and carboxylato oxygen, respectively, and the third glycinate acts as a bridge through its two carboxylato oxygens linking the two molybdenum atoms. Compound 2 is also a binuclear Mo? S complex with two nitrilotriacetate ligands, each of which is coordinated to a molybdenum atom via its two β‐carboxylato oxygens and a nitrogen atom. Simultaneously, each molybdenum atom in 1 and 2 is chelated to a terminal oxygen and two bridging sulfurs to complete the octahedral configuration. Their catalytic activities in the reduction from C₂H₂ to C₂H₄ as well as other binuclear Mo? S? polycarboxylate complexes, a [Fe₄S₄] single cubane and a chainlike Mo? Fe? S compound were investigated and it was found that 1 exhibited relatively good catalytic activity. Copyright © 2007 John Wiley & Sons, Ltd.     

Stokes型积分——微分方程的Galerkin近似

本文讨论一类具有Stokes方程结构的积分一微分方程的Galerkin近似，论证了近似解的存在唯一性，并分别导出速度和压力近似解的最优阶L_2模误差估计。