Synthesis,Antiproliferative, and Antiplatelet Activities of Oxime‐Containing 3,4‐Dihydroquinolin‐2(1H)‐One Derivatives

Some oxime‐containing 3,4‐dihydroquinolin‐2(1H)‐one derivatives were synthesized and evaluated for their antiplatelet and antiproliferative activities. These compounds were synthesized via alkylation of hydroxyl precursors followed by the reaction with NH₂OH. The preliminary assays indicated that (Z)‐7‐[2‐(4‐fluorophenyl)‐2‐(hydroxyimino)ethoxy]‐3,4‐dihydroquinolin‐2(1H)‐one (13c) is the most active against U46619 induced platelet aggregation with an IC₅₀ value of 3.51 μM. For the inhibition of AA‐induced aggregation, (E)‐6‐[2‐(hydroxyimino)propoxy]‐3,4‐dihydroquinolin‐2(1H)‐one (15 ) is the most potent with an IC₅₀ value of 1.85 μM. These oxime‐containing 3,4‐dihydroquinolin‐2(1H)‐one derivatives were inactive against thrombin induced platelet aggregation with an IC₅₀ value of greater than 26.78 μM. For the antiproliferative activity, most of these oxime‐containing 3,4‐dihydroquinolin‐2(1H)‐one derivatives were inactive while (Z)‐7‐[2‐(hydroxyimino)‐2‐(naphthalen‐2‐yl)ethoxy]‐3,4‐dihydroquinolin‐2(1H)‐one (13a) exhibited only marginal activities with GI₅₀ value of 7.63, 7.34 and 6.36 μM against the growth of NPC‐TW01, NCI‐H661, and Jurkat respectively.     

A New Strategy to Identify and Eliminate the Inner Filter Effects by Outer Filter Technique

To identify and eliminate the inner filter effects (IFEs), prepositive and side cells containing absorbents are fixed beside the fluorescer contained cell. In this way, excitation and emission lights can be quenched by primary and secondary outer filter effects respectively, depending on absorbent concentration and cell length. Herein the quenching of emission fluorescence caused by IFEs can be equally reduced by outer filter effects (OFEs) and the interference of IFEs was eliminated. This approach was experimentally used for identifying the interaction mode and mechanism between BSA and nanoAg. Results showed that the quenching of BSA fluorescence and synchronous fluorescence mainly attributes to IFEs, instead of static/dynamic fluorescent quenching. In view of the above, the elimination of the interference of IFEs by the design of OFEs plays an important part in the precise application of fluorescence detector.     

Observation of collective atomic recoil motion in a degenerate fermion gas

We demonstrate collective atomic recoil motion with a dilute, ultracold, degenerate fermion gas in a single spin state. By utilizing an adiabatically decompressed magnetic trap with an aspect ratio different from that of the initial trap, a momentum-squeezed fermion cloud is achieved. With a single pump pulse of the proper polarization, we observe, for the first time, multiple wave-mixing processes that result in distinct collective atomic recoil motion modes in a degenerate fermion cloud. Contrary to the case with Bose condensates, no pump-laser detuning asymmetry is present.     

Ankyrin repeat and SOCS box containing protein 4 (Asb-4) colocalizes with insulin receptor substrate 4 (IRS4) in the hypothalamic neurons and mediates IRS4 degradation

Background

Cigarette smoking enhances the risk of stroke. However, the underlying molecular mechanisms are largely unknown. The present study established an in vivo rat secondhand cigarette smoking (SHS) model and examined the hypothesis that SHS upregulates endothelin receptors with increased cerebrovascular contraction via the Raf/extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinases (MAPK) pathway.

Results

Rats were exposed to SHS for up to 8 weeks. The cerebral artery vasoconstriction was recorded by a sensitive myograph. The mRNA and protein expressions for endothelin receptors in cerebral arteries were studied by real-time PCR and Western blot. Compared to fresh air exposed rats, cerebral arteries from SHS rats exhibited stronger contractile responses (P < 0.05) mediated by endothelin type A (ET_A) receptors. The expressions of mRNA and protein for ET_A receptors in the cerebral arteries from SHS rats were higher (P < 0.05) than that in control. SHS did not affect endothelin type B (ET_B) receptor-mediated contractions, mRNA or protein levels. The results suggest that SHS upregulates ET_A, but not ET_B receptors in vivo. After SHS exposure, the mRNA levels of Raf-1 and ERK1/2, the protein expression of phosphorylated (p)-Raf-1 and p-ERK1/2 were increased (P < 0.05). Raf-1 inhibitor, GW5074 suppressed the enhanced ET_A receptor-mediated contraction, mRNA and protein levels induced by SHS. In addition, GW5074 inhibited the SHS-caused increased mRNA and phosphorylated protein levels of Raf-1 and ERK1/2, suggesting that SHS induces activation of the Raf/ERK/MAPK pathway.

Conclusions

SHS upregulates cerebrovascular ET_A receptors via the Raf/ERK/MAPK pathway, which provides novel understanding of mechanisms involved in SHS-associated stroke.     

一种检测低浓度化学需氧量的双波长光谱方法

提出了一种基于双波长光谱法快速测定低污染水样的化学需氧量(COD)的方法.结果表明:对低COD样品采用440和560 nm双波长的测定方法可有效地提高可靠性和检测灵敏性.该法的灵敏度(即定量检测下限)为8.6 mg·L-1;在低COD范围标样(≤100mg·L-1)的准确性对比测定相对标准误差在2%～15%的范围内.另外,该方法不需要用标准样品进行校正,可以通过光谱法检测消解液中重铬酸根的消耗直接计算出样品的COD值,使实验步骤得到了进一步的简化.     

混沌量子克隆算法求解认知无线网络频谱分配问题

对认知无线网络中的频谱进行有效分配是实现动态频谱接入的关键技术.考虑3次用户对频谱的需求和分配的公平性,给出了频谱分配的数学模型,并将其转换为以最大化网络收益为目标的带约束优化问题,进而提出一种采用混沌量子克隆优化求解的认知无线网络频谱分配算法, 并证明了该算法以概率1收敛.最后,通过仿真实验比较了本文算法与颜色敏感图着色算法、基于遗传算法的频谱分配、基于量子遗传算法的频谱分配的性能.结果表明:本文算法性能较优, 能更好地实现网络收益最大化. 关键词： 混沌量子克隆算法 认知无线网络 频谱分配     

O2流量对磁控溅射N掺杂TiO2薄膜成分及晶体结构的影响

利用直流脉冲磁控溅射方法在室温下通过改变O₂流量制备具有不同晶体结构的N掺杂TiO₂薄膜,利用台阶仪、X射线光电子能谱仪、X射线衍射仪、紫外-可见分光光度计等设备对薄膜沉积速率、化学成分、晶体结构、禁带宽度等进行分析.结果表明:所制备的薄膜元素配比约为TiO_1.68±0.06N_0.11±0.01,N为替位掺杂,所有样品退火前后均未形成Ti—N相结构,N掺杂TiO₂薄膜的沉积速率、晶体结构等主要依赖于O₂流量.在O₂流量为2 sccm时,N掺杂TiO₂薄膜沉积速率相对较高,薄膜为非晶态结构,但薄膜内含有锐钛矿(anatase)和金红石(rutile)相晶核,退火后薄膜呈anatase和rutile相混合结构,禁带宽度仅为2.86 eV.随着O₂流量的增加,薄膜沉积速率单调下降,退火后样品禁带宽度逐渐增加.当O₂流量为12 sccm时,薄膜为anatase相择优生长,退火后呈anatase相结构,禁带宽度为3.2 eV.综合本实验的分析结果,要在室温条件下制备晶态N掺杂TiO₂薄膜,需在高O₂流量(＞10 sccn)条件下制备. 关键词： 2薄膜')" href="#">N掺杂TiO₂薄膜 磁控溅射 化学配比 晶体结构     

High pulse energy mode-locked multicore photonic crystal fiber laser

A high pulse energy passively mode-locked fiber laser operating in the all-normal dispersion regime is demonstrated. The gain material is an Yb-doped multicore photonic crystal fiber with 18 cores in array-type geometry. Robust and self-starting mode locking is achieved using a fast semiconductor saturable absorber mirror. The laser generates 180?nJ chirped pulses at a 14.48?MHz repetition rate for an average power of 2.6?W. The 1.15?ps output pulses are compressed to 690?fs outside the cavity.     

Analysis and improvement of Laguerre-Gaussian beam position estimation using quadrant detectors

Laguerre-Gaussian beams have become increasingly important in areas like optical manipulation and quantum communications. A good understanding of the accuracy and sensitivity of their displacement measurements are required for these applications. In this Letter, we present a method to analyze and compare the performance of these measurements, and an estimation technique is proposed to improve their measurement accuracies. The effectiveness of our methods is verified by simulation studies.     

Influence of substrate temperature on surface textured ZnO:Al films etched with NaOH solution

Transparent conducting Al-doped ZnO (ZnO:Al, AZO) thin films with good optical and electrical characteristics were prepared by direct current pulse magnetron sputtering. Textured surfaces of AZO films were obtained by etching with NaOH solution successfully and the effect of substrate temperature on the surface texture was investigated. The surface is covered with craters after etching with 5% NaOH solution, and the crater diameter decreases gradually as substrate temperature increases. For AZO film deposited at 270, the crater diameters is 0.5-1 μm, which is an effective surface texture for light trapping.