Erythrocyte electrokinetic behavior under low ionic strength conditions

Under low ionic strength conditions, a linear approximation of the electrostatic potential within the charged polyelectrolyte layer coating the membrane bilayer leads to overestimation of the red cell electrokinetic mobility. Considering the full non linearized Poisson-Boltzmann and Navier-Stokes equations relative to the erythrocyte system, the deviation at low ionic strength between theoretical and experimental values for the cell mobility then almost disappears. A weak glycocalyx expansion may account for the remaining differences. However, this result is in contrast with previously suggested explanations involving an important stretching of the polyelectrolyte chains to interpret the “artificial” erythrocyte aggregation at low ionic strength. Under such conditions cell aggregation is probably induced by haemolysate adsorption onto the erythrocyte membrane and is unlikely to be the consequence of a strong interfacial rearrangement.     

A Series of Collaborations Between Various Pharmaceutical Companies and Regulatory Authorities Concerning the Analysis of Biomolecules Using Capillary Electrophoresis

An international project team (including members from US, Canada and UK) has been formed from a number of interested biopharmaceutical companies and regulatory authorities to conduct a cross-organisation collaboration exercise. The results from this exercise demonstrate the robustness of CE-SDS across eight different organisations that used instruments of the same equipment model, the same reagents, and the same methodology. Data generated from the analysis of a series of molecular weight markers showed very good precision with regards to relative migration time (RMT) both within and between organisations. The apparent molecular weight of bovine serum albumin (BSA) was measured with good precision to within approximately 2% RSD across the participants. A representative IgG sample showed similar results with regards to relative migration time of its 3 main components, IgG Light Chain, IgG Non-glycosylated Heavy Chain, and IgG Heavy Chain. Fractional peak area for each peak also showed good agreement, with less than 9% RSD for all peaks. This exercise will facilitate both increased regulatory and industrial opinion of CE for biopharmaceutical analysis.CE in the Biotechnology & Pharmaceutical Industries: 7th Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides and Small Molecules, Montreal, Canada, August 12–16, 2005     

Adaptation of PTB’s analytical modelling for TDCR–Cherenkov activity measurements at LNHB

Designed for triple to double coincidence ratio measurements based on liquid scintillation, the three-photomultipliers detection system can also be applied for Cherenkov counting using aqueous solutions. For activity determination, a specific modelling of Cherenkov light emission has to be constructed. For that purpose, the analytical modelling first developed at PTB was adapted to account for the physical features of the detection system used at LNHB. The first results are presented in the case of activity measurements of two high-energy β^?-emitters (⁹⁰Y and ⁸⁹Sr). The analytical modelling was also tested for the standardization of ⁶⁸Ge in a solution of ⁶⁸Ge/⁶⁸Ga in equilibrium in the framework of a BIPM international comparison in 2014.     

Large and moderate deviations principles for kernel estimators of the multivariate regression

In this paper, we prove large deviations principle for the Nadaraya-Watson estimator and for the semi-recursive kernel estimator of the regression in the multidimensional case. Under suitable conditions, we show that the rate function is a good rate function. We thus generalize the results already obtained in the one-dimensional case for the Nadaraya-Watson estimator. Moreover, we give a moderate deviations principle for these two estimators. It turns out that the rate function obtained in the moderate deviations principle for the semi-recursive estimator is larger than the one obtained for the Nadaraya-Watson estimator.      

Initial growth of the water layer on (1 x 1)-oxygen-covered Ru(0001) in comparison with that on bare Ru(0001)

The initial growth of a water (D2O) layer on (1 x 1)-oxygen-covered Ru(0001) has been studied in comparison with that on bare Ru(0001) by means of temperature-programmed desorption (TPD) and infrared reflection absorption spectroscopy (IRAS). Although water molecules adsorbed on both bare and (1 x 1)-oxygen-covered Ru(0001) commonly tend to form hydrogen bonds with each other when mobility occurs upon heating, the TPD and IRAS measurements for the two surfaces exhibit distinct differences. On (1 x 1)-oxygen-covered Ru(0001), most of the D2O molecules were desorbed with a peak at 160 K, even at submonolayer coverage, as condensed water desorption. The vibration spectra of adsorbed D2O also showed broad peaks such as a condensed water phase, from the beginning of low coverage. For submonolayer coverage, in addition, we found a characteristic O-D stretching mode at around 2650 cm(-1), which is never clearly observed for D2O on bare Ru(0001). Thus, we propose a distinctive water adsorption structure on (1 x 1)-oxygen-covered Ru(0001) and discuss its influence on water layer growth in comparison with the case of D2O on bare Ru(0001).     

Self-chemical ionization of diethylzinc

The self-chemical ionization of diethylzinc is examined by Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry and semiempirical molecular orbital calculations. Electron impact of diethylzinc neutral produces the radical cation, C(4)H(15)Zn(+) (m/z x 122), which reacts further with the neutral (C(2)H(5))(2)Zn to give the following product ions: Zn(+) (m/z x 64), C(2)H(5)Zn(+) (m/z x 93), C(4)H(9)Zn(+) (m/z x 121), C(4)H(11)Zn(2)(+) (m/z x 187), and C(6)H(15)Zn(2)(+) (m/z x 215). To determine the structure and pathways for production of these ions, monoisotopic (12)C(4)H(15)(64)Zn(+), (64)Zn(+) and (12)C(2)H(5)(64)Zn(+) were individually isolated and reacted with the neutral background. We also performed semiempirical molecular orbital calculations (ZINDO/1). The molecular orbital calculations and experimental data are consistent in predicting that the ethyl group on the diethylzinc cation carries the positive charge. Copyright 1999 John Wiley & Sons, Ltd.     

A model to characterize the effect of cross phase modulation on polarization in an optical fiber transmission system

This paper presents a model that described the process of inter-influence between cross phase modulation (XPM) and polarization effects such as polarization mode dispersion (PMD) and polarization dependant loss (PDL) in an optical fiber communication system. Previous papers have discussed PMD-PDL and PMD-XPM effects in separate models, but in this work we developed a model that is capable of characterizing and evaluating PMD, PDL and XPM simultaneously. From the model, we found that PMD and PDL can be affected by XPM. The presence of XPM changed the vector directions of PMD and PDL in Stokes space.