PYRIMIDINE DIMER FORMATION AND GERMINATION OF UV-IRRADIATED SPORES OF DICTYOSTELIUM DISCOIDEUMNC–4 ANDys–13

Abstract— Survival, UV-photoproducts and germination of UV-irradiated spores of Dictyostelium discoi-deum were studied on two strains,NC–4 andys–13. The spores ofNC–4 are about 35 times more resistant to UV thanys–13 spores at 10% survival. Pyrimidine dimers were formed in UV-irradiated spores in both strains. No photoproducts other than pyrimidine dimers were detected. The formation of pyrimidine dimers in spores was about 2% in both strains at 800 J/m₂. In the germination of spores, the conversion of spores into swollen spores was not affected by UV in both strains, but the emergence of amoebae from the swollen spores was suppressed, which was more distinctive inys–13 spores than inNC–4 spores. The emerged amoebae from the UV-irradiatedNC–4 spores were viable, while those from theys–13 spores were inviable even when they succeeded in emergence.     

Semiconductor-mediated oxidative dimerization of 1-naphthols with dioxygen and O-demethylation of the enol-ethers by SnO2 without dioxygen

Oxidative dimerization of 1-naphthols 1 with dioxygen in the presence of a semiconductor, such as SnO₂, ZrO₂, or activated charcoal, as a catalytic mediator takes place selectively to give the corresponding 2,2′-binaphthols 2 or 2,2′-binaphthyl-1,1′-quinones 3 in excellent yields without light irradiation. The catalytic activity of SnO₂ could be fully restored by appropriate reactivation treatment after the oxidation. In addition, SnO₂ without dioxygen catalyzes selective O-demethylation of the enol-ethers 3 to 4.     

Isotope effects and the mechanism of epoxidation of cyclohexenone with tert-butyl hydroperoxide

The mechanism of the epoxidation of 2-cyclohexen-1-one with tert-butyl hydroperoxide mediated by DBU was studied by a combination of experimental kinetic isotope effects (KIEs) and theoretical calculations. A large 12C/13C (k(12C)/k(13C)) isotope effect of approximately equal to 1.032 was observed at the C3 (beta) position of cyclohexenone, while a much smaller 12C/13C isotope effect of 1.010 was observed at the C2 (alpha) position. Qualitatively, these results are indicative of nucleophilic addition to the enone being the rate-limiting step. Theoretical calculations support this interpretation. Transition structures for the addition step lead to predicted isotope effects that approximate the experimental values, while the predicted isotope effects for the ring-closure step are not consistent with the experimental values. The calculations correctly favor a rate-limiting addition step but suggest that the barriers for the addition and ring-closure steps are crudely similar in energy. The stereochemistry of these epoxidations is predicted to be governed by a preference for an initial axial addition, and the role of this preference in experimental diastereoselectivity observations is discussed.     

Backward error analysis for linearizations in heavily damped quadratic eigenvalue problem

Heavily damped quadratic eigenvalue problem (QEP) is a special type of QEPs. It has a large gap between small and large eigenvalues in absolute value. One common way for solving QEP is to linearize the original problem via linearizations. Previous work on the accuracy of eigenpairs of not heavily damped QEP focuses on analyzing the backward error of eigenpairs relative to linearizations. The objective of this paper is to explain why different linearizations lead to different errors when computing small and large eigenpairs. To obtain this goal, we bound the backward error of eigenpairs relative to the linearization methods. Using these bounds, we build upper bounds of growth factors for the backward error. We present results of numerical experiments that support the predictions of the proposed methods.     

SEMIDISCRETIZATION IN SPACE OF NONLINEAR DEGENERATE MRABOLIC EQUATIONS WITH BLOW-UP OF THE SOLUTIONS

1. IntroductionLet n be a bounded domain in AN with smooth boundary Off. We consider thefollowing initial boundary value problem:where 6, p are positive constants and "o(x) is a nonnegative bounded continuous function on fi.When N = 1 and 5 ~ 2, the problem arises in a model for the resistive diffusion of aforce--free magnetic field in a plasma confined between two walls in one dimension (see[5], [8], [9], [10] and [14]). Equation (1) also describes the evolution of the curvatureof a locally…     

Reversed-phase liquid chromatographic separation of enantiomers on polysaccharide type chiral stationary phases

The direct chiral separation by chiral stationary phases (CSPs) is one of the most important techniques to analyze enantiomeric purity as well as to get enantiomerically pure material quickly. Among various types of CSPs, polysaccharide type CSPs are well known by their versatility and durability. They are not only effective under normal-phase conditions, but also under reversed-phase conditions. In order to get a good separation under the reversed-phase conditions, it is the key to choose an appropriate mobile phase. For example, a simple mixture of water/acetonitrile or water/methanol are sufficient for a neutral analyte, while it is necessary to use an acidic solution instead of water for an acidic analyte and a solution of a chaotropic salt (or a basic solution) for a basic analyte, respectively. The paper also presents lists of more than 350 separation examples that include 22 validated methods for drug analyses from serum, plasma, or urine samples on polysaccharide type CSPs under reversed-phase conditions.     

Molecular dynamics simulations of self-organized polyicosahedral Si nanowire

A novel polyicosahedral nanowire is spontaneously formed in a series of annealing molecular dynamics simulations of liquid Si inside a nanopore of 1.36 nm in diameter. The polyicosahedral Si nanowire is stable even in a vacuum up to about 77% of the melting temperature of bulk Si. Our structural energy calculations reveal that the polyicosahedral nanowire is energetically advantageous over the pentagonal one for a wire whose diameter is less than 6.02 nm, though the latter has been recently proposed as the lowest energy wire. These results suggest the possibility of the formation of a new stable polyicosahedral Si nanowire.     

Three-dimensional dynamics of a vortex array in rotating superfluid

This work studies numerically three-dimensional formation of a vortex array in a rotating cylindrical vessel. The formation process and the resulting equilibrium pattern are affected by pinning sites on the vessel’s bottom.     

An ampere-like law for displacement vector field and near-field optical microscopy

We make up a novel and simple theory for near field and near-field optical microscopy (NOM). Our theory is composed of two parts. The first is a formulation to calculate the scattered near field of light by a small dielectric. We state that a wavenumber-vector-independent picture appears in the theory of the near field, and we find that this is expressed by an Ampere-like law for the displacement vector field. The second part is a formulation of field intensity for far field observation and near field observation from a unified point of view. We suggest a theoretical formula for the field intensity corresponding to the NOM image and demonstrate how to understand the relation between the near field and this image.