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Heiko Packeiser Chanyuen Lim Balaji Balagurunathan Jinchuan Wu Hua Zhao 《Applied biochemistry and biotechnology》2013,169(2):695-700
An extremely simple and effective colony PCR procedure is established for both gram-negative and gram-positive bacteria, yeasts, and microalgae. Among the four lysis buffers examined, Y-PER is observed to be more effective than Tris/EDTA, 0.2 % SDS, and 10 mM EDTA in the extraction of PCR-quality genomic DNA from those microorganisms. Vortexing or pipetting agitation of the cells in Y-PER for 5–10 s was sufficient to release genomic DNA for all the test bacteria and yeasts, and most microalgae. Additional incubation at 98 °C for 5 min for further cell disruption was essential only for Chlorella vulgaris due to its notoriously rigid cell wall. 相似文献
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B. Gonzalez Prieto J. Lim A. Mariën K. Rosseel J. A. Martens J. Van den Bosch J. Neuhausen A. Aerts 《Journal of Radioanalytical and Nuclear Chemistry》2014,302(1):195-200
Understanding polonium evaporation from lead-bismuth eutectic (LBE) is required for the design of nuclear installations that use liquid LBE as coolant or spallation target. In the present study we measured the time-dependent release of polonium from LBE samples in Ar/5 %H2 and Ar between room temperature and 500 °C. Our experiments revealed that the majority of polonium in the samples evaporated according to established temperature correlations for the Henry constant of polonium in LBE. However a small fraction of polonium in the LBE behaved differently, causing a relatively large but transient polonium release at the start of evaporation experiments. We showed that this volatile fraction of polonium was located near the sample surface and was formed after prolonged exposure of the samples to air at room temperature. We speculate that the peculiar evaporation behavior of this surface polonium is caused by enrichment and association with an oxide layer. 相似文献
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Seung-Beum Park Byeong-Guk Kim Jeong-Yeon Kim Tae-Hwan Jung Dong-Gun Lim Jae-Hwan Park Jae-Gwan Park 《Applied Physics A: Materials Science & Processing》2011,102(1):169-172
We fabricated a nanowire-based field-emission display (FED) device on a 2.5D substrate using a photolithography, lift-off, thermal-evaporation, and plasma-etching process. We first fabricated a 3×3 array of microholes (diameter = 400???m and depth = 50???m) on a Si substrate and fabricated ZnO nanowires inside the microholes by using a thermal CVD process. The field-emission pattern image of the 3×3 array of microholes was clearly apparent. The threshold emission field was ca. 5.6?V/??m and we obtained considerable brightness when the applied voltage was 1900?V (i.e. 6.3?V/??m). Because the fabrication processes used in this study are standard semiconductor fabrication routes, the study suggests the feasibility of mass producing a nanowire-based FED device. 相似文献
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In order to provide statistically reliable information of a wind energy site, accurate analysis on the atmospheric stability and climate characteristics in a certain area is a prerequisite. Two 2-D ultrasonic anemometers and one cup anemometer, located perpendicular to the prevailing wind direction, were used to measure the atmospheric wind environment at a height of 4.5 m in coastal region of the Wol–Ryong, Jeju, South Korea. The study is aiming to understand the atmospheric stability about a coastal region, and the effect of roughness length. We calculate the Monin–Obukhov length for division of atmospheric stability about unstable regime, neutral regime and stable regime. The distribution of diurnal Monin–Obukhov length is highly sporadic in the coastal region due to the effect of radiant heat from the surface or other environmental effects. In order to calculate the roughness length in coastal region, three different methods are applied in terms of the surface roughness, flow fluctuation and gust wind, which are called logarithmic profile, standard deviation and gust factor methods. In the study, the atmospheric stability was insignificant when applying these three methods. In the results, three different roughness length scales sufficiently showed the effect of obstacle and surface conditions around the measurement position. On the basis of an overall analysis of the short-term data measured in the Wol–Ryong area, Jeju Island, it is concluded that for the development of future wind energy resources, the Wol–Ryong site could be a good candidate for a future wind energy site. 相似文献
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