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61.
Anisha Gokarna Yong Hwan Kim Yong-Hoon Cho Min Su Lee In Cheol Kang Hyun Kyu Park Min Gon Kim Bong Hyun Chung 《Optical Review》2006,13(4):288-291
We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy
(SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3
dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives
termed Prolinker. We have also tried to probe into the well-known “doughnut effect” observed in fluorescence images of proteins
using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary
of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge
than at the center in the confocal image. 相似文献
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The development and the use of the diamond anvil cell for Mössbauer Spectroscopy (MS) to pressures nearing 100 GPa are discussed. Three types of cells and their typical performance are given. Pressure calibration, hydrostatic media, gasketing, collimation, -ray absorption, and sample size for MS are reviewed. New MS results showing hysteresis in the room temperature high pressure transition in iron and showing the rapid rise ofT
N in NiI2 with pressure are presented. 相似文献
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Bonner BE Buchanan JA Clement JM Corcoran MD Krishna NM Kruk JW Miettinen HE Moss RM Mutchler GS Nessi-Tedaldi F Nessi M Phillips GC Roberts JB Stevenson PM Tonse SR Birman A Chung SU Etkin A Fernow RC Kirk H Protopopescu SD Willutzki H Hallman T Madansky L Bar-Yam Z Dowd J Kern W King E Mayes BW Pinsky LS 《Physical review D: Particles and fields》1990,41(1):13-16
66.
We introduce a class of optimization problems, calleddynamic location problems, involving the processing of requests that occur sequentially at the nodes of a graphG. This leads to the definition of a new parameter of graphs, called the window indexWX(G), that measures how large a window into the future is needed to solve every instance of the dynamic location problem onG optimally on-line. We completely characterize this parameter:WX(G)k if and only ifG is a weak retract of a product of complete graphs of size at mostk. As a byproduct, we obtain two (polynomially recognizable) structural characterizations of such graphs, extending a result of Bandelt. 相似文献
67.
W Taylor 《The Journal of the Acoustical Society of America》1988,83(2):415-422
The objective of this paper is to highlight gaps of information regarding mechanisms of vascular, neurological, and musculo-skeletal damage caused by vibration. Also addressed is evidence that high noise level may act synergistically to the development of vibration syndrome of the hand and arm. Areas of research currently active in psychophysical and neurophysiological investigations to increase our understanding of tactile and spatial discrimination are discussed. Although the importance of sensory loss or "fine touch" is understood, there is neither a proven objective scientific test with which the syndrome can be diagnosed nor is there a scale of damage assessment. Determining the exact role of the central nervous system in assessing damage from vibration is difficult in view of nonspecific symptoms reported from eastern Europe and from Japan. To complicate matters still further, there is the possibility that repeated, rapid mechanical movements of the hand and arm associated with handling heavy tools produce carpal tunnel syndrome but that the injury is not directly attributed to vibration. Therefore, it follows that there could exist an element of carpal tunnel syndrome in many vibration syndrome cases. 相似文献
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