Investigation of volatile biomarkers in liver cancer blood using solid-phase microextraction and gas chromatography/mass spectrometry

Solid-phase microextraction (SPME) followed by gas chromatography/mass spectrometry (GC/MS) was used for the detection of liver cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to the determination of volatiles in human blood. Between the liver cancer group (n = 19) and the normal group (n = 18), positive rates of 19 volatile compounds among the total of 47 detected were found to be different with statistical significance (p < 0.05, chi-squared test). We suggested hexanal, 1-octen-3-ol and octane, of the 19 compounds, as biomarkers of liver cancer with clinical diagnostic value. The sensitivity and specificity of 94.7% and 100% for hexanal, 84.2% and 100% for 1-octen-3-ol, and 89.5% and 100% for octane were obtained, respectively, after the cutoff values had been properly established. These results show that SPME-GC/MS is a simple, rapid and sensitive method for the investigation of volatile disease markers in human blood.     

A serum metabolomic investigation on hepatocellular carcinoma patients by chemical derivatization followed by gas chromatography/mass spectrometry

The purpose of this study was to investigate the serum metabolic difference between hepatocellular carcinoma (HCC, n = 20) male patients and normal male subjects (n = 20). Serum metabolome was detected through chemical derivatization followed by gas chromatography/mass spectrometry (GC/MS). The acquired GC/MS data was analyzed by stepwise discriminant analysis (SDA) and support vector machine (SVM). The metabolites including butanoic acid, ethanimidic acid, glycerol, L-isoleucine, L-valine, aminomalonic acid, D-erythrose, hexadecanoic acid, octadecanoic acid, and 9,12-octadecadienoic acid in combination with each other gave the strongest segregation between the two groups. By applying these variables, our method provided a diagnostic model that could well discriminate between HCC patients and normal subjects. More importantly, the error count estimate for each group was 0%. The total classifying accuracy of the discriminant function tested by SVM 20-fold cross validation was 75%. This technique is different from traditional ones and appears to be a useful tool in the area of HCC diagnosis. Copyright (c) 2008 John Wiley & Sons, Ltd.     

Recognition of forcible curvature in circular DNA by human Topoisomerase I

A series of forcible curved circular DNAs (cDNAs) were prepared to investigate the recognition features of human Topoisomerase I (hTopo I). The IC(50) can be modulated by the curvature degrees of cDNA. In addition, preferential bindings of hTopo I to cDNA with high curvature have been observed by AFM and EMSA.     

PVDF梳状换能器接收非线性兰姆波的实验研究*

该文基于PVDF压电薄膜对梳状换能器进行设计制作,并将该换能器应用于接收非线性兰姆波信号实验研究。首先通过输出矢量和方法分析PVDF梳状换能器的工作特性,然后将设计制作的PVDF梳状换能器应用于铝板中非线性兰姆波信号的接收,并与传统压电陶瓷换能器经斜劈接收的非线性兰姆波信号进行比较分析。实验结果表明,两种换能器所接收到的信号幅值随传播距离的变化趋势相近,线性增长的积累效应表征结果相似,且PVDF梳状换能器可以对基波和二次谐波信号同时响应。除此之外,PVDF梳状换能器用于接收非线性兰姆波信号更加稳定。因此,PVDF梳状换能器有望应用于复杂构件的在线检测与监测研究。     

新核素238Th的鉴别

利用60MeV/u的¹⁸O离子束轰击天然铀靶,通过多核子转移反应生成重丰中子新核素²³⁸Th.观测到了²³⁸Th的子体²³⁸Paγ活性的生长、衰变行为,鉴别了²³⁸Th的存在,并测得它的半衰期为(9.4±2.0)min.同时,发现了一条能量为(89.0±0.3)keV、半衰期为(8.9±1.5)min的新γ射线,指定为²³⁸Th的衰变γ射线.     

雷公藤精（Wilforgine）的2D NMR研究

用２Ｄ ＮＭＲ（＾１Ｈ－＾１ＨＣＯＳＹ，＾１Ｈ－＾１３Ｃ ＣＯＳＹ，ＴＯＣＳＹ，ＮＯＥＳＹ和ＣＯＬＯＣ）技术研究了从雷公藤中分离到的雷公藤精，对＾１Ｈ和＾１３Ｃ谱进行了归属。     

一种环烯醚萜甙（8—O—acetyl—shanzhiside methylester）的2D NMR

利用二维核磁共振技术对从独一味植物中分离的的环烯醚萜甙化合物进行了＾１Ｈ和＾１３Ｃ ＮＭＲ的归属，并确定其糖与甙的接点。     

强激光远场光束质量参数的测试

 提出漫射红外成像-多点标校测量方法,用于测量强激光远场光束质量参数。在激光远场距离处设置漫反射靶板,用成像探测器摄取经靶面漫射的脉冲强激光光斑图像;在靶面中心处挖小孔,孔后放置能量探测器实时测量激光脉宽和峰值功率。同时对整个激光光斑图像进行能量定标,进而得出远场脉冲强激光的实际空间能量/功率分布、总能量,以及相应的光束质量参数。应用该测量方法,对高能TEA CO₂激光进行测量研究,测得其远场光束截面半径为80.2 mm,发散角为1.55 mrad。     

Cyclometalated Gold(III)-Hydride Complexes Exhibit Visible Light-Induced Thiol Reactivity and Act as Potent Photo-Activated Anti-Cancer Agents

The specific gold-sulfur binding interaction renders gold complexes as promising anti-cancer agents that can potentially overcome cisplatin resistance; while their unbiased binding towards non-tumoral off-target thiol-proteins has posed a big hurdle to clinical application. Herein we report that cyclometalated gold(III) complexes bearing hydride ligands are highly stable towards thiols in the dark but can efficiently dissociate the auxiliary hydride moiety and generate a gold-thiol adduct when excited with visible light. In consequence, the photo-activated gold(III) complexes potently inhibited thioredoxin reductase in association with up to >400-fold increment of photocytotoxicity (vs. dark condition) without deactivation by serum albumin and along with strong anti-angiogenesis activity in zebrafish embryos. Importantly, the gold(III)-hydride complexes could be activated by two-photon laser irradiation at the phototherapeutic window as effectively as blue-light irradiation.