全文获取类型
收费全文 | 150篇 |
免费 | 10篇 |
专业分类
化学 | 119篇 |
数学 | 18篇 |
物理学 | 23篇 |
出版年
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 2篇 |
2019年 | 6篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 14篇 |
2015年 | 6篇 |
2014年 | 6篇 |
2013年 | 16篇 |
2012年 | 16篇 |
2011年 | 15篇 |
2010年 | 6篇 |
2009年 | 5篇 |
2008年 | 11篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1981年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1959年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 421 毫秒
71.
Adsorption and recovery issues of recombinant monoclonal antibodies in reversed‐phase liquid chromatography†
下载免费PDF全文
![点击此处可从《Journal of separation science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Szabolcs Fekete Alain Beck Elsa Wagner Karine Vuignier Davy Guillarme 《Journal of separation science》2015,38(1):1-8
The poor recovery of large biomolecules is a well‐known issue in reversed‐phase liquid chromatography. Several papers have reported this problem, but the reasons behind this behavior are not yet fully understood. In the present study, state‐of‐the‐art reversed‐phase wide‐pore stationary phases were used to evaluate the adsorption of therapeutic monoclonal antibodies. These biomolecules possess molar mass of approximately 150 000 g/mol and isoelectric points between 6.6 and 9.3. Two types of stationary phases were tested, the Phenomenex Aeris Widepore (silica based), with 3.6 μm superficially porous particles, and the Waters Acquity BEH300 (ethylene‐bridged hybrid), with 1.7 μm fully porous particles. A systematic investigation was carried out using 11 immunoglobulin G1, G2, and G4 antibodies, namely, panitumumab, natalizumab, cetuximab, bevacizumab, trastuzumab, rituximab, palivizumab, belimumab, adalimumab, denosumab, and ofatumumab. All are approved by the Food and Drug Administration and the European Medicines Agency in various therapeutic indications and are considered as reference antibodies. Several test proteins, such as human serum albumin, transferrin, apoferritin, ovalbumin, and others, possessing a molar mass between 42 000 and 443 000 g/mol were also evaluated to draw reliable conclusions. The purpose of this study was to find a correlation between the adsorption of monoclonal antibodies and their physicochemical properties. Therefore, the impact of isoelectric point, molar mass, protein glycosylation, and hydrophobicity was investigated. The adsorption of intact antibodies on the stationary phase was significantly higher than that of proteins of similar size, isoelectric point, or hydrophobicity. The present study also demonstrates the unique behavior of monoclonal antibodies, contributing some unwanted and unpredictable strong secondary interactions. 相似文献
72.
A general and convenient two-step synthetic method has been developed for the preparation of a novel class of aminoalkyl-phosphine type compounds, which involves nucleophilic ring-opening of cyclic sulfate esters. The ring-opening step was performed using several different aliphatic and aromatic amines to produce aminoalkyl sulfates that were reacted with LiPPh2 to give the corresponding P,N-ligands. The desymmetrization procedure affords an easy route to synthesize enantiomerically pure pentane-2,4-diyl based P,N-ligands with a highly tunable structure. The ligands derived from primary amines have a stereogenic N-atom that can be useful in asymmetric catalytic syntheses. 相似文献
73.
The quantization of mirror curves to toric Calabi–Yau threefolds leads to trace class operators, and it has been conjectured that the spectral properties of these operators provide a non-perturbative realization of topological string theory on these backgrounds. In this paper, we find an explicit form for the integral kernel of the trace class operator in the case of local \({{\mathbb{P}}^1 \times {\mathbb{P}}^1}\), in terms of Faddeev’s quantum dilogarithm. The matrix model associated to this integral kernel is an \({O(2)}\) model, which generalizes the ABJ(M) matrix model. We find its exact planar limit, and we provide detailed evidence that its \({1/N}\) expansion captures the all genus topological string free energy on local \({{\mathbb{P}}^1 \times {\mathbb{P}}^1}\). 相似文献
74.
The structure of aqueous sodium hydroxide solutions: a combined solution x-ray diffraction and simulation study 总被引:1,自引:0,他引:1
Megyes T Bálint S Grósz T Radnai T Bakó I Sipos P 《The Journal of chemical physics》2008,128(4):044501
To determine the structure of aqueous sodium hydroxide solutions, results obtained from x-ray diffraction and computer simulation (molecular dynamics and Car-Parrinello) have been compared. The capabilities and limitations of the methods in describing the solution structure are discussed. For the solutions studied, diffraction methods were found to perform very well in describing the hydration spheres of the sodium ion and yield structural information on the anion's hydration structure. Classical molecular dynamics simulations were not able to correctly describe the bulk structure of these solutions. However, Car-Parrinello simulation proved to be a suitable tool in the detailed interpretation of the hydration sphere of ions and bulk structure of solutions. The results of Car-Parrinello simulations were compared with the findings of diffraction experiments. 相似文献
75.
Daruhzi gnes E. Szarka Szabolcs Hthelyi va Simndi Bla Gyurjn Istvn Lszl Mikls Szke va Lemberkovics va 《Chromatographia》2008,68(1):71-76
A GC-MS method has been developed for the qualitative analysis of sterol and triterpene (terpenoid) constituents of Ononis spinosa L. (spiny restharrow) root without derivatization. β-Sitosterol, campesterol, stigmasterol, stigmastan-3,5-diene sterol compounds and the triterpene derivatives β-amyrin and α-onocerin were identified. A validated GC-FID quantitative method was developed for measuring β-sitosterol, the main sterol component, in various extracts of this plant, obtained with organic solvents and by supercritical fluid extraction. The extracts were cleaned by saponifying and then the β-sitosterol was quantified in the non-saponifiable fractions by GC-FID with an internal standard. In addition, the relative concentrations of the other terpenoids were also determined. The β-sitosterol content in the non-saponifiable solvent extraction fractions was 0.19–5.5%, that of the supercritical fractions were 4.8–9.2%, depending on the experimental conditions. The hexane and the pilot scale SFE extracts were considered as main sources of terpenoids (71.8%; 93.3%, respectively). 相似文献
76.
Tobias Biberger Szabolcs Makai Dr. Zhong Lian Dr. Bill Morandi 《Angewandte Chemie (International ed. in English)》2018,57(23):6940-6944
Among all metathesis reactions known to date in organic chemistry, the metathesis of multiple bonds such as alkenes and alkynes has evolved into one of the most powerful methods to construct molecular complexity. In contrast, metathesis reactions involving single bonds are scarce and far less developed, particularly in the context of synthetically valuable ring‐closing reactions. Herein, we report an iron‐catalyzed ring‐closing metathesis of aliphatic ethers for the synthesis of substituted tetrahydropyrans and tetrahydrofurans, as well as morpholines and polycyclic ethers. This transformation is enabled by a simple iron catalyst and likely proceeds via cyclic oxonium intermediates. 相似文献
77.
Szabolcs Sipos 《Tetrahedron letters》2009,50(16):1844-930
A one-pot Strecker reaction using various alkyl, arylalkyl and arylnitriles is developed. Aldimine alanes were generated in situ from nitriles by the addition of diisobutylaluminium hydride, and were converted into the corresponding imines on reaction with (S)-(−)-1-phenylethylamine. Nucleophilic addition to the imines in the presence of catalytic triethylamine, using acetone cyanohydrin as a cyanide source, provided α-aminonitriles. 相似文献
78.
JPC – Journal of Planar Chromatography – Modern TLC - 相似文献
79.
Prof. Dr. László Kótai Szabolcs Bálint István Gács Györgyi Lakatos András Angyal Prof. Dr. Raj. N. Mehrotra 《无机化学与普通化学杂志》2012,638(2):279-281
The minimal occupancy level (θmin) of the clathrate lattice of gas molecules is defined as the number of guest molecules in the host clathrate lattice, which can stabilize the thermodynamically unstable empty cage by covering the energy demand of the transformation of hexagonal ice into empty clathrate lattice (ΔHtrans). The θmin values for chlorine hydrate were determined from the n = f(p)T=const. relationship and the average molar intercalation heat of chlorine in the type I clathrate lattice was also calculated for both type of cavities. 相似文献