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101.
102.
[structure: see text] The design, synthesis, and biological evaluation of seven totally synthetic analogues of the antitumor agent (+)-discodermolide are reported. Saturation of the terminal diene system, alteration of the substituents on the lactone, and alkylation of the C7-hydroxyl group reveal significant structure-activity relationships. 相似文献
103.
[structure: see text]. The solution structure of (+)-discodermolide (1) has been determined via 1- and 2-D NMR techniques in conjunction with Monte Carlo conformational analysis. Taken together, the results demonstrate that in solution (+)-discodermolide occupies a helical conformation remarkably similar to the solid state conformation. 相似文献
104.
Rajarshi Roy D.S. Elliot D. Meschede F.M. Pipkin S.J. Smith 《Chemical physics letters》1982,93(6):603-607
We report the observation of collisional narrowing of the Q branch of the Raman spectrum for the (000) → (100) transition in CO2 at very low pressures. The minimum linewidth is reached at ≈250 Torr. An estimate for the difference in rotational constants of the (100) state and the ground state is obtained. The narrowing of the linewidth and the changes in line-shape are interpreted in terms of velocity changing collisions and rotationally inelastic collisions between the CO2 molecules. 相似文献
105.
Smith JC Siu KW Rafferty SP 《Journal of the American Society for Mass Spectrometry》2004,15(5):629-638
The investigation of protein quaternary structure, protein-cofactor, and protein-ligand interactions by mass spectrometry is often limited by the fragility of such interactions under experimental conditions. To develop more gentle conditions of perhaps general use, we used as a model for study the oxygenase domain of murine inducible nitric oxide synthase (iNOS), which is homodimeric, binds heme and tetrahydrobiopterin H(4)B cofactors, and the substrate L-arginine. The energetics of the collisions in q2 and in the lens region of the mass spectrometer were manipulated for varying the degree of solvation around the non-covalently bound ions. Furthermore, the number of low-energy collisions in the collision cell of the instrument was varied, focusing and dampening the ion beam. Under gentle source collision conditions, and using multiple low-energy collisions in the collision cell of the mass spectrometer, dimers of the iNOS oxygenase domain containing heme, H(4)B, and arginine were observed intact after electrospraying at pH values near neutrality; a mutant of this protein (Trp188 --> Phe) was monomeric and did not bind cofactors. The pH dependence of the iNOS oxygenase domain under acidic conditions was also studied; while heme remained bound to the protein between pH 2.5 and 4.0, the dimeric structure was disrupted. Our findings confirm that non-covalently bound macromolecular complexes are retained and observable using electrospray mass spectrometry under the appropriate experimental conditions. 相似文献
106.
Turney K Drake TJ Smith JE Tan W Harrison WW 《Rapid communications in mass spectrometry : RCM》2004,18(20):2367-2374
Nanoparticles for the extraction of peptides and subsequent analysis using atmospheric pressure matrix-assisted laser desorption/ionization (APMALDI) have been evaluated. The atmospheric pressure source allows for particles to be directly introduced in the liquid matrix, minimizing sample loss and analysis time. Described in this work are two sample preparation procedures for liquid APMALDI analysis: a C18 functionalized silica nanoparticle for hydrophobic extractions, and an aptamer functionalized magnetite core nanoparticle for rapid, affinity extractions. The C18 particles provide a non-selective support for rapid profiling applications, while the aptamer particles are directed towards reducing the complexity in biological samples. The aptamer functionalized particles provide a more selective analyte-nanoparticle interaction whereby the tertiary structure of the analyte becomes more critical to the extraction. In both cases, the liquid APMALDI matrix provides a support for ionization, and acts as the releasing agent for the analyte-particle interaction. Additionally, analyte enrichment was possible due to the large surface-to-volume ratio of the particles. The experiments conducted with functionalized nanoparticles, in an atmospheric pressure liquid matrix, present a basis for further methodologies and utilities of silica nanoparticles to be developed. 相似文献
107.
Dihydroxyphenylalanine (DOPA), its methyl ester (DOPAM) and the N-acetylated derivative of the ester (DOPAMNA) are found to undergo rapid oxidation in air-saturated alkaline solution. Some of the products of oxidation exhibit fluorescent emission in the 300-500 nm spectral range and their excitation-emission spectra have been determined in acidic and alkaline aqueous solutions. The spectral distributions and positions of the maxima depend on the pH of the solution. Excitation-emission maxima associated with the protonated phenolic form of the compounds occur at shorter wavelengths than those of the conjugate base. At some pH values the phenolic forms of these molecules are excited and undergo rapid deprotonation in the excited state; as a consequence, emission is observed from the phenolate anion. The fluorescence excitation-emission spectrum of an authentic sample of 3,4-dihydroxycinnamic (caffeic) acid has also been determined and features of the fluorescence spectra of the principal oxidation products are consistent with the presence of 3,4-hydroxycinnamoyl compounds in solutions of oxidized DOPAM and DOPAMNA. 相似文献
108.
Chemistry is described for the fabrication of DNA arrays on gold surfaces. Alkanethiols modified with terminal aldehyde groups are used to prepare a self-assembled monolayer (SAM). The aldehyde groups of the monolayer may be reacted with amine-modified oligonucleotides or other amine-bearing biomolecules to form a Schiff base, which may then be reduced to a stable secondary amine by treatment with sodium cyanoborohydride. The surface modifications and reactions are characterized by polarization modulation Fourier transform infrared reflection absorption spectroscopy (PM-FTIRRAS), and the accessibility, binding specificity, and stability of the DNA-modified surfaces are demonstrated in hybridization experiments. 相似文献
109.
Summary A method is described for the combustion of halogen containing organic compounds in milligram quantities in a closed system. It has been found to have greater applicability than existing techniques. After combustion the halogen is determined by an amperometric titration, which permits the determination of Cl–, Br– and I– either singly or in admixture. The accuracy is probably better than existing micro methods for single halides and comparable with these methods for mixed halides.
Zusammenfassung Eine Methode zur Verbrennung von Milligrammengen halogenhaltiger organischer Verbindungen im geschlossenen System wurde beschrieben. Sie hat einen größeren Anwendungsbereich als bisher bekannte Verfahren. Nach der Verbrennung wird das Halogen durch eine amperometrische Titration bestimmt, womit Chlorid, Bromid und Jodid sowohl einzeln wie in Gemischen bestimmt werden können. Die Genauigkeit seheint besser zu sein als bei bekannten Mikromethoden für die Bestimmung einzelner Halogene und etwa gleich gut wie bei Methoden zur Analyse von Halogengemischen.
Résumé On décrit une méthode de combustion en vase clos, des composés organiques contenant des halogènes en quantités de l'ordre du milligramme. Cette méthode a été trouvée comme ayant un domaine d'applications plus grand que celui des techniques existant déjà. Après combustion, l'halogène est dosé par titrage ampérométrique, ce qui permet le dosage de Cl–, Br– et I– isolés ou en mélange. La précision est probablement meilleure que celle des microméthodes existant pour les halogénures isolés et comparable à celle de ces méthodes pour les mélanges d'halogénures.相似文献
110.