反相高效液相色谱-串联质谱法测定尿液中儿茶酚胺和肾上腺素(英文)

The measurement of urine catecholamine and metanephrine concentrations is important for biochemical screening and diagnosis of pheochromocytoma.The goal of this work was to develop a simple liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for determining catecholamines and metanephrines in urine to replace an existing liquid chromatographic method using electrochemical detection.Urine samples were prepared using Oasis weak-cation-exchange cartridges.The eluate was analyzed on an Agilent ZORBAX Eclipse Plus Phenyl-Hexyl column in 3 min.Adrenaline,noradrenaline,dopamine,metanephrine,normetanephrine,and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring(MRM).No evidence of ion suppression was observed.The assay was linear up to 5μmol/L for adrenaline,5μmol/L for noradrenaline,6.1μmol/L for dopamine,5.6μmol/L for metanephrine,and 34.6μmol/L for normetanephrine,with lower limits of quantification of 5,5,12,6 and 7nmol/L,respectively.The intra-day and inter-day precisions for all analytes ranged from 0.59%to 4.64%and1.98%to 4.80%,respectively.External quality assurance samples were assayed and showed excellent agreement with the target values.This simple method provides an improved assay for determining urine catecholamines and metanephrines.     

μ介子寿命与μ-e对称性的实验限度

μ介子有反常作用,破坏了μ-e对称性。本文讨论了反常作用对弱作用过程的影响,分析了反常作用的电荷无关性和r₅变换性质与μ蜕变弱作用常数的重正化之间的联系,证明了重正化后G_V=G,G_A=εG,ε=1+δ。利用这一结果,可以解释矢量流守恒假定下普适V-A费米弱作用理论在解释μ介子寿命时遇到的困难。从理论和实验的比较中定出δ=0.04,这是由μ蜕变定出的μ-e对称性的实验限度。     

A Lattice Model for Bidirectional Pedestrian Flow on Gradient Road

Ramps and sloping roads appear everywhere in the built environment. It is obvious that the movement pattern of people in the sloping path may be different as compared with the pattern on level roads. Previously, most of the studies, especially the mathematical and simulation models, on pedestrian movement consider the flow at level routes. This study proposes a new lattice model for bidirectional pedestrian flow on gradient road. The stability condition is obtained by using linear stability theory. The nonlinear analysis method is employed to derive the modified Korteweg-de Vries (mKdV) equation, and the space of pedestrian flow is divided into three regions: the stable region, the metastable region, and the unstable region respectively. Furthermore, the time-dependent Ginzburg—Landan (TDGL) equation is deduced and solved through the reductive perturbation method. Finally, we present detailed results obtained from the model, and it is found that the stability of the model is enhanced in uphill situation while reduced in downhill situation with increasing slope.     

多重分形谱研究溶胶-凝胶SiO2疏水减反膜的激光预处理作用

采用溶胶-凝胶提拉技术制备了SiO₂疏水减反膜.使用Nd：YAG激光（波长为1064nm,脉宽为7.5ns）采用“R on 1”方式对所得膜层进行了激光预处理.在预处理前后采用“1 on 1”方式考察了薄膜的激光损伤阈值的变化,使用原子力显微镜（AFM）观察了薄膜的表面形貌的变化,并使用多重分形谱（MFS）方法分析了薄膜分形结构的变化.结果表明经过激光预处理后薄膜的抗激光损伤阈值有了明显提高,均方根表面粗糙度（R_q）稍有减小,膜面变平整,多重分形谱宽度收缩,分形区间的分布均匀性改善.这说明经过激光预处理后薄膜表面微结构趋向规整,使之能够承受更强的激光的辐照.同时也说明借助多重分形谱可以获得更多薄膜表面结构变化的信息,多重分形谱是探索强激光对光学薄膜辐照作用机理的一个十分有用的方法. 关键词： 2疏水减反膜')" href="#">SiO₂疏水减反膜 激光预处理 多重分形谱 激光损伤阈值     

动量为6.8BeV/cπ-介子与核子的非弹性作用

本工作共积累了317个非弹性作用事例。作用中的次级带电粒子在(π^--N)质心系中的角分布与动量分布等和前人结果一致,并且基本上可以用边缘碰撞机制来说明。对带有次级质子的事例作了如下分析:(1)计算全部次级粒子(质子除外)在它们自己系统中的总能量ω。作ω分布,并与边缘碰撞1π交换近似计算的理论曲线比较,在ω=1.5BeV附近观察到有突出于理论曲线之外的高峯,并对峯区内事例进行了初步分析。(2)综合我们的和联合原子核研究所的数据,计算了次级质子在镜象系的非弹性系数K_з,并使K_з值的分布与根据Барашенков等人理论计算的K_з分布曲线作了比较。此外,还试从靶质量观点出发,利用Duller-Walker的F图方法,对不同K_з值的各类事例进行了分析和讨论。