Cyclin A-Cdk2 Phosphorylates BH3 only Protein Bad in vitro and in vivo

Increasing evidence suggests that Cyclin A-Cdk2 activity is required in the apoptosis process induced by various stimuli. To determine a specific substrate of Cyclin A-Cdk2 for apoptosis, in this study, we carried out anin vitro kinase assay using immunoprecipitated complex Cyclin A-Cdk2 as an enzyme source, and recombinant protein GST-Bad as a substrate. Our study showed that Bad was clearly phosphorylated by Cyclin A-Cdk2 in vitro. To examine whether protein Bad can also be phosphorylated by Cyclin A-Cdk2 kinase in vivo, we transiently overexpressed protein Bad with Cyclin A or Cdk2-dn, a dominant negative version of Cdk2, in Hela cells and determined the phosphorylation status of protein Bad. The test showed that protein Bad was clearly phosphorylated in Cyclin A overexpressed cells,but not in Cdk2-dn or mock transfectent. Moreover, etoposide also caused the phosphorylation of endogenetic Bad. In conclusion, here we provide first time evidence that protein Bad can be a substrate of Cyclin A-Cdk2 apoptosis for in vitro and in vivo.     

系列柱形薄壁腔靶制备工艺研究

为了从实验上深入研究超热电子产生规律，从而减少或抑制超热电子对惯性约束聚变（ＩＣＦ）的危害，我们制备了一系列薄壁腔靶，以供实验研究。本文详细地描述了柱形薄壁腔靶的制备工艺。利用ＮＧ－１０４型精密单向纵切车床，采用金刚石刀具车削，提高心轴质量，表表粗糙度可达０．１μｍ。采用电镀和磁控溅射二种方法镀膜，为了使腔靶壁厚均匀，在镀膜时，必须使心轴匀速旋转。利用磁控溅射在腔靶外表面再涂上１μｍ左右厚的二氧化硅，以提高超薄壁腔靶的强度和自立能力。在腐蚀心轴时，必须仔细控制酸的浓度，防止在腐蚀时因产生气泡太多，太快而使腔靶破裂。用Ｘ射线照相法和扫描电子显微镜测量腔靶的几何参数。制备系列柱形薄壁腔靶达到指标为：壁厚范围２～３０μｍ，壁厚均匀性小于１０％，表面粗糙度０．２～０．３μｍ。最后介绍了在“神光Ⅰ”上打靶结果。结果表明，实验值与理论值符合较好。     

Kramers rate formula with coordinate-dependent mass

We study in this paper the classical stationary fission rate including a coordinate-dependent mass and a realistic potential. The results show that our numerical rate formula is a good approximation to the realistic Langevin simulation even if the nuclear temperature is not smaller than the fission barrier.     

Analysis and design of mechanical crack arrestors for gas pipeline

Mechanical arrestors are frequently used in gas transmission pipelines to prevent the possibility of long running cracks. These arrestors have the effect of reducing the pipe from opening as the crack passes. This decreases the available crack driving force and, as a result, crack arrest may take place. This essentially is a second line of defence against catastrophic failure in the event crack initiation cannot always be prevented. A novel analyzing method presented in this paper has been developed to investigate the suitability of these crack arrestors. This is based on a fluid/structure/fracture interaction package, PFRAC. A dynamic finite element approach has been developed for a three dimension curved beam with initial plane curvature, which has been implemented in PFRAC to simulate the behaviour of the arrestor. The contact conditions between the pipe wall and the arrestor, along with the various computational procedures, are described. Several numerical results for a cracked pipe with arrestors are presented and compared with those for pipes without arrestors.     

箔式高阻值低压锰铜压阻应力计的设计及动态标定

设计了低压锰铜压阻传感器 ,并通过平板撞击实验标定了该传感器在 0 .3～ 9.4GPa范围内的压阻关系。标定曲线在 0 .3～ 1.5GPa范围内是线性的 ,对应的是传感器的弹性状态 ,而在 1.5～ 9.4GPa范围内是二次曲线。标定曲线的拐点在 1.5GPa附近 ,这与理论分析和他人的实验结果相接近。     

DRIVING FORCE AND DEFORMATION ANALYSIS FOR DYNAMIC CRACK PROPAGATION IN GAS PIPELINES UNDER DIFFERENT BOUNDARY CONDITIONS

In the light of a growing need for fracture control of rapid crack propagation(RCP)ingas pipelines,a program PFRAC(Pipeline FRacture Analysis Code)has been developed to analysethc various events.In this paper,by using PFRAC for the simulation of axial crack propagation in gaspipelines,a number of dynamic analysis issues rclating to boundary effects for uncracked and crackedpipes are investigated.This indicates that the boundary conditions along the length of the pipe play animportant role for fracture analysis in the pipe.In contrast.the boundary condutions at thc ends of along pipeline have little effect on the dynamic fracture events.     

壁面温度在多参数下对平板边界层的影响研究

为了得到壁面温度在不同来流速度、不同湍流强度条件下对边界层转捩与减阻的影响规律,本文采用Transitionk-kl-ω模型对低来流速度下无压力梯度的光滑平板进行了数值模拟。结果表明,随着来流速度的升高,壁温升高所起到的减阻效果更好,即高来流速度对壁面温度更为敏感。当来流处于中高湍流强度下时,壁温升高能起到推迟转捩的作用,且随着湍流强度的升高,转捩推迟的效果越好,但减阻效果正好相反;当来流处于低湍流强度下时,壁温升高会使得转捩提前发生。壁温升高抑制了边界层内流体的脉动程度,使得层流的稳态不易被破坏,流动更加稳定;同时,壁温升高使得边界层内流体的速度梯度减小,从而降低了壁面摩擦系数,故壁温升高能起到推迟边界层转捩与减阻的作用。     

三维问题的随机界面元法

在研究岩土工程的整体稳定可靠度分析及二维随机界面元法的基础上，建立了等参随机界面元法的数学模型，推导了基本公式，编制了计算程序，以均质边坡的抗滑可靠度分析作为数值算例，并就计算结果进行了分析和探讨．     

Multifunctional Nano‐Bioprobes Based on Rattle‐Structured Upconverting Luminescent Nanoparticles

Lanthanide‐doped upconversion nanoparticles (UCNPs) have shown great promise in versatile bioapplications. For the first time, organosilica‐shelled β‐NaLuF₄:Gd/Yb/Er nanoprobes with a rattle structure have been designed for dual‐modal imaging and photodynamic therapy (PDT). Benefiting from the unique rattle structure and aromatic framework, these nanoprobes are endowed with a high loading capacity and the disaggregation effect of photosensitizers. After loading of β‐carboxyphthalocyanine zinc or rose Bengal into the nanoprobes, we achieved higher energy transfer efficiency from UCNPs to photosensitizers as compared to those with conventional core–shell structure or with pure‐silica shell, which facilitates a large production of singlet oxygen and thus an enhanced PDT efficacy. We demonstrated the use of these nanoprobes in proof‐of‐concept X‐ray computed tomography (CT) and UC imaging, thus revealing the great potential of this multifunctional material as an excellent nanoplatform for cancer theranostics.     

寡肽自组装纳米材料研究进展

肽类自组装是自然界普遍存在的分子自组装现象之一,研究其自组装行为对理解生命现象、仿生制备以及构建功能材料等具有重要的意义.寡肽由于合成简单,结构组成明确,同时具有良好的生物相容性、可控的降解性,其组装体在药物/基因控制释放、细胞培养、组织工程支架材料及生物矿化等领域具有很大的应用前景.本文概述了近年来寡肽自组装的研究进展,系统介绍了线性和环状寡肽的分子结构设计、自组装体形貌及组装机理以及组装体在生物医学等领域的应用.