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61.
62.
This article is concerned with the existence and robust stability of an equilibrium point that related to interval inertial Cohen–Grossberg neural networks. Such condition requires the existence of an equilibrium point to a given system, so the existence and uniqueness of the equilibrium point are emerged via nonlinear measure method. Furthermore, with the help of Halanay inequality lemma, differential mean value theorem as well as inequality technique, several sufficient criteria are derived to ascertain the robust stability of the equilibrium point for the addressed system. The results obtained in this article will be shown to be new and they can be considered alternative results to previously results. Finally, the effectiveness and computational issues of the two models for the analysis are discussed by two examples. © 2016 Wiley Periodicals, Inc. Complexity 21: 459–469, 2016  相似文献   
63.
Cell-based microfluidic devices have attracted interest for a wide range of applications. While optical cell counting and flow cytometry-type devices have been reported extensively, sensitive and efficient non-optical methods to detect and quantify cells attached over large surface areas within microdevices are generally lacking. We describe an electrical method for counting cells based on the measurement of changes in conductivity of the surrounding medium due to ions released from surface-immobilized cells within a microfluidic channel. Immobilized cells are lysed using a low conductivity, hypotonic media and the resulting change in impedance is measured using surface patterned electrodes to detect and quantify the number of cells. We found that the bulk solution conductance increases linearly with the number of isolated cells contributing to solution ion concentration. The method of cell lysate impedance spectroscopy is sensitive enough to detect 20 cells microL(-1), and offers a simple and efficient method for detecting and enumerating cells within microfluidic devices for many applications including measurement of CD4 cell counts in HIV patients in resource-limited settings. To our knowledge, this is the most sensitive approach using non-optical setups to enumerate immobilized cells. The microfluidic device, capable of isolating specific cell types from a complex bio-fluidic and quantifying cell number, can serve as a single use cartridge for a hand-held instrument to provide simple, fast and affordable cell counting in point-of-care settings.  相似文献   
64.
Calmodulin is an EF hand calcium binding protein. Its binding affinities to various protein/peptide targets often depend on the conformational changes induced by the binding of calcium. One such target is melittin, which binds tightly to calmodulin in the presence of calcium, and inhibits its function. Chemical cross-linking combined with Fourier transform ion cyclotron resonance mass spectrometry has been employed to investigate the coordination of calmodulin and melittin in the complex at different concentrations of calcium. This methodology can be used to monitor structural changes of proteins induced by ligand binding, and study the effects these changes have on non- covalent interactions between proteins. Cross-linking results indicate that the binding place of the first melittin in the calcium free calmodulin form is the same as in the calcium loaded calmodulin/melittin complex.  相似文献   
65.
Phytochemical investigation of Sarcococca saligna by extensive bioassay‐guided fractionation resulted in the isolation of the pregnane‐type steroidal alkaloids 1 – 15 , i.e. of the five new compounds 1 – 5 and the ten known alkaloids 6 – 15 . The structures of the new alkaloids salignenamide C ( 1 ), salignenamide D ( 2 ), 2β‐hydroxyepipachysamine D ( 3 ), salignenamide E ( 4 ), and salignenamide F ( 5 ) were elucidated with the help of modern spectroscopic techniques, while the known alkaloids axillarine C ( 6 ), axillarine F ( 7 ), sarcorine ( 8 ), N3‐demethylsaracodine ( 9 ), saligcinnamide ( 10 ), salignenamide A ( 11 ), vaganine A ( 12 ), axillaridine A ( 13 ), sarsalignone ( 14 ), and sarsalignenone ( 15 ) were identified by comparing their spectral data with those reported earlier. Inhibition of electric‐eel acetylcholinesterase (EC 3.1.1.7) and horse‐serum butyrylcholinesterase (EC 3.1.1.8) by alkaloids 1 – 15 were investigated. These new cholinesterase inhibitors may act as potential leads in the discovery of clinically useful inhibitors for nervous‐system disorders, particularly by reducing memory deficiency in Alzheimer's disease patients by potentiating and effecting the cholinergic transmission process. These compounds were found to inhibit both enzymes in a concentration‐dependent fashion with the IC50 values ranging from 5.21–227.92 μM against acetylcholinesterase and 2.18–38.36 μM against butyrylcholinesterase.  相似文献   
66.
The fermion propagator in an arbitrary covariant gauge can be obtained from the Landau gauge result via a Landau–Khalatnikov–Fradkin transformation. This transformation can be written in a practically useful form in both configuration and momentum space. It is therefore possible to anticipate effects of a gauge transformation on the propagator’s analytic properties. These facts enable one to establish that if a critical number of flavours for chiral symmetry restoration and deconfinement exists in noncompact QED3, then its value is independent of the gauge parameter. This is explicated using simple forms for the fermion–photon vertex and the photon vacuum polarisation. The illustration highlights pitfalls that must be avoided in order to arrive at valid conclusions. Landau gauge is seen to be the covariant gauge in which the propagator avoids modification by a non-dynamical gauge-dependent exponential factor, whose presence can obscure truly observable features of the theory.  相似文献   
67.
Silver nanoparticles (Ag-NPs) were synthesized using a facile green chemistry synthetic route. The reaction occurred at ambient temperature with four reducing agents introduced to obtain nanoscale Ag-NPs. The variables of the green synthetic route, such as acidity, concentration of starting materials, and molar ratio of reactants were optimized. Dispersing agents were employed to prevent Ag-NPs from aggregating. Advanced instrumentation techniques, such as X-ray powder diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), ultraviolet–visible spectroscopy (UV–vis), and phase analysis light scattering technique (ZetaPALS) were applied to characterize the morphology, particle size distribution, elemental composition, and electrokinetic behavior of the Ag-NPs. UV–vis spectra detected the characteristic plasmon at approximately 395–410 nm; and XRD results were indicative of face-centered cubic phase structure of Ag. These particles were found to be monodispersed and highly crystalline, displaying near-spherical appearance, with average particle size of 10.2 nm using citrate or 13.7 nm using ascorbic acid as reductants from particle size analysis by ZetaPALS, respectively. The rapid electrokinetic behavior of the Ag was evaluated using zetapotential (from −40 to −42 mV), which was highly dependant on nanoparticle acidity and particle size. The current research opens a new avenue for the green fabrication of nanomaterials (including variables optimization and aggregation prevention), and functionalization in the field of nanocatalysis, disinfection, and electronics.  相似文献   
68.
The objective of this work is to develop and validate spectrophotometric method for the determination of piroxicam in commercial dosage forms. The method is based on the chelation of the drug with Fe(III) to form pink coloured metal chelate at room temperature which absorbs maximally at 504 nm. Beer's law is obeyed over the concentration range of 8–160 μg mL?1 (A = 1.07 × 10?3 + 7.75 × 10?3 C). Under the optimized experimental conditions, proposed method is validated as per the International Conference on Harmonisation guidelines. The limits of detection and quantitation for the proposed method are 0.775 and 2.348 μg mL?1, respectively. The proposed method has been successfully applied to the determination of piroxicam in commercial dosage forms. The results are compared with the reference El‐Ries et al. spectrophotometric method.  相似文献   
69.
An analytical method has been developed for the preconcentration of rare earth elements (REEs) in seawater for their determination by inductively coupled plasma optical emission spectrometry (ICP‐OES). An indigenously synthesized chelating resin was used for the preconcentration of (REEs) which was based on immobilization of fluorinated β‐diketone group on solid support styrene divinyl benzene. Sample solutions (adjusted to optimized pH) were passed through a polyethylene column packed with 250 mg of the resin. Experimental conditions consisting of pH, sample flow rate, sample volume and eluent concentration were optimized. The established method has been applied for the preconcentration of light, medium and heavy REEs in coastal sea water samples for their subsequent determination by (ICP‐OES). Percentage recoveries of La, Ce, Nd, Sm, Eu, Gd, Dy, Er, Yb and Lu were ≥ 95%, a preconcentration factor of 200 times, and relative standard deviations < 5% were achieved.  相似文献   
70.
The suitability of three different retention models to predict the retention times of poly(ethylene glycol)s (PEGs) in gradient and isocratic chromatography was investigated. The models investigated were the linear (LSSM) and the quadratic solvent strength model (QSSM). In addition, a model describing the retention behaviour of polymers was extended to account for gradient elution (PM). It was found that all models are suited to properly predict gradient retention volumes provided the extraction of the analyte specific parameters is performed from gradient experiments as well. The LSSM and QSSM on principle cannot describe retention behaviour under critical or SEC conditions. Since the PM is designed to cover all three modes of polymer chromatography, it is therefore superior to the other models. However, the determination of the analyte specific parameters, which are needed to calibrate the retention behaviour, strongly depend on the suitable selection of initial experiments. A useful strategy for a purposeful selection of these calibration experiments is proposed.  相似文献   
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