A microfluidic "baby machine" for cell synchronization

Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the "baby machine" was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle. However, the existing "baby machine" is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic "baby machine" in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety of cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic "baby machine" by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase.     

Risk arbitrage in the Nikkei put warrant market of 1989–1990

This paper discusses the Nikkei put warrant market in Toronto and New York during 1989–1990. Three classes of long term American puts were traded which when evaluated in yen are ordinary, product and exchange asset puts, respectively. Type I do not involve exchange rates for yen investors. Type II, called quantos, fix in advance the exchange rate to be used on expiry in the home currency. Type III evaluate the strike and spot prices of the Nikkei Stock Average in the home currency rather than in yen. For typically observed parameters, type I are theoretically more valuable than type II which in turn are more valuable than type III. In late 1989 and early 1990 there were significant departures from fair values in various markets. This was a market with a set of complex financial instruments that even sophisticated investors needed time to learn about to price properly. Investors in Canada were willing to buy puts at far more than fair value based on historical volatility. In addition, US investors overpriced type II puts fixed in dollars rather than the type I's in yen. This led to cross border and US traded (on the same exchange) low risk hedges. The market's convergence to efficiency (that is, all puts priced within transaction cost bands) took about one month after the introduction of the US puts in early 1990 leading to significant profits for the hedgers.     

Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: Mass spectrometric structural characterisation and cloning of precursor cDNAs

Amphibian skin secretions contain a plethora of bioactive compounds, many of which are understood to act to deter ingestion by predators. Bradykinins in particular are constitutively expressed in many amphibian skin secretions, mediating a variety of effects including hyperalgesia and contraction of gastric smooth muscle. Using a variety of proteomic techniques (high-performance liquid chromatography (HPLC) separation, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), and quadrupole time-of-flight tandem mass spectrometry (Q-TOF-MS/MS)) the current study identified 13 bradykinin-like peptides in the skin secretions of Phyllomedusa hypochondrialis azurea, including several new C-terminally extended isoforms (VPPGFTPFRLT, VHypPGFTPFRQT) and a novel phyllokinin-like peptide (RPPGFTPFRVY). Identification of the cDNA sequences encoding these peptides led to the deduction that the peptides were derived from differential post-translational processing and modification of five different precursors. Such an event emphasises the metabolic efficiency of peptide production in amphibian venom, with multiple products perhaps selective to different receptors in a variety of predators generated from a single precursor. An unusual modification was also recognised in the present study, with several bradykinin-like peptides featuring hydroxyprolination of the first proline residue rather than the commonly targeted second. This alteration may be mediated by the structural organisation of N-terminal amino acids prior to precursor processing.     

The structural and stereogenic properties of pentaerythritoxy-bridged cyclotriphosphazene derivatives: spiro-spiro, spiro-ansa and ansa-ansa isomers

Reactions of pentaerythritol with hexachlorocyclotriphosphazene, N3P3Cl6, and gem-disubstituted cyclotriphosphazene derivatives, N3P3Cl4R2 [R = Ph, NHBu(t) or (OCH2CF2CF2CH2O)0.5] gave a series of pentaerythritol-bridged derivatives linked spiro-spiro, spiro-ansa and ansa-ansa. The structures and stereogenic properties of the products were characterised by X-ray crystallography and 31P NMR spectroscopy on addition of the chiral solvating agent, (S)-(+)-2,2,2-trifluoro-1-(9-anthryl)ethanol. Molecules with spiro-spiro and spiro-ansa bridged gem-disubstituted cyclophosphazenes [R = Ph, NHBu(t) or (OCH2CF2CF2CH2O)0.5] are found to be chiral and exist as racemates. Molecules with ansa-ansa bridged cyclophosphazenes [R = Cl or (OCH2CF2CF2CH2O)0.5] have been characterised for the first time and are shown to have meso configurations. Analysis of crystal structure data shows that the six-membered chair form of the spiro rings and the eight-membered boat-chair form of the ansa rings in the bridged compounds are similar to analogous spiro and ansa exocyclic ring conformations of 1,3-propanedioxy-derivatives of cyclophosphazenes.     

The taming of absorption: generating a constant intensity beam in a lossy medium

We report the first observation (to our best knowledge) of a constant intensity, quasi-Bessel/nondiffracting beam in an absorbing medium generated by a novel optical element, "exicon," or exponential intensity axicon. Such absorption-compensated and diffraction-resistant beams can find applications in illumination, remote sensing, free-space communications, imaging in biological tissues, nonlinear optics, and other situations where absorption and diffraction hinder light propagation.     

Counterexamples in calculus of variations in L∞ through the vectorial Eikonal equation

We show that, for any regular bounded domain $\mathrm{\Omega }?{\mathbb{R}}^n$, $n=2,3$, there exist infinitely many global diffeomorphisms equal to the identity on ?Ω that solve the Eikonal equation. We also provide explicit examples of such maps on annular domains. This implies that the ∞-Laplace system arising in vectorial calculus of variations in $L^{\infty }$ does not suffice to characterise either limits of p-Harmonic maps as $p\to \infty$ or absolute minimisers in the sense of Aronsson.     

On the smoothing properties of the SIMPLE pressure-correction algorithm

A local mode Fourier analysis is used to assess the suitability of the SIMPLE pressure-correction algorithm to act as a smoother in a multigrid method. The necessary ellipticity of the Navier-Stokes equations and their discrete representation are established. The theoretical analysis is compared with practical results.     

Vibrational relaxation of KrF* and XeCl* by rare gases

A steady-state, chemiluminescence technique has been used to measure effective rate constants for vibrational relaxation as a function of vibrational level for KrF^* in collisions with He, Ne, and Ar and XeCl^* with Ar. The effective rate constants reported include contributions to relaxation due to intersystem crossing between theB andC states, in addition to direct relaxation within theB state. The relevance of these results to the understanding of previous measurements in KrF and XeCl lasers is discussed.