Quantitative real-time measurements of DNA hybridization with alkylated nonoxidized silicon nanowires in electrolyte solution

The quantitative, real-time detection of single-stranded oligonucleotides with silicon nanowires (SiNWs) in physiologically relevant electrolyte solution is demonstrated. Debye screening of the hybridization event is circumvented by utilizing electrostatically adsorbed primary DNA on an amine-terminated NW surface. Two surface functionalization chemistries are compared: an amine-terminated siloxane monolayer on the native SiO2 surface of the SiNW, and an amine-terminated alkyl monolayer grown directly on a hydrogen-terminated SiNW surface. The SiNWs without the native oxide exhibit improved solution-gated field-effect transistor characteristics and a significantly enhanced sensitivity to single-stranded DNA detection, with an accompanying 2 orders of magnitude improvement in the dynamic range of sensing. A model for the detection of analyte by SiNW sensors is developed and utilized to extract DNA-binding kinetic parameters. Those values are directly compared with values obtained by the standard method of surface plasmon resonance (SPR) and demonstrated to be similar. The nanowires, however, are characterized by higher detection sensitivity. The implication is that SiNWs can be utilized to quantitate the solution-phase concentration of biomolecules at low concentrations. This work also demonstrates the importance of surface chemistry for optimizing biomolecular sensing with silicon nanowires.     

Determination of hexavalent chromium (Cr(VI)) in plastics using organic-assisted alkaline extraction

An organic-assisted alkaline extraction method was developed for the determination of hexavalent chromium (Cr(VI)) in plastics. The solubilization of polymer as a pre-step of the alkaline extraction provided good extraction efficiency of Cr(VI) from the sample. The optimization of the experimental conditions affecting the extraction and UV–vis spectrophotometric analysis was accomplished by evaluating the recovery rate of Cr(VI) through the analysis of Cr(VI) in in-house polymer reference materials (RMs). With the proposed method, most of the Cr(VI) in polymers was released within a short extraction time of 30 min and the Cr(III)-DPCO complex can be kept stable for 24 h. The heating for the extraction of the Cr(VI) was not necessary. The optimal pH of the final solution was fixed at 2.0. The proposed extraction method was applied successfully for the determination of Cr(III) and Cr(VI) in spiked samples. The practical applicability of this new method was evaluated through the analysis of Cr(VI) in in-house polymer RMs. The good linearity was demonstrated at desired concentrations of the range 0–3.3 mg L⁻¹. The detection limits were quite low, varying from 0.0061 to 0.0285 mg L⁻¹. The recovery of Cr(VI) was between 97 and 106%, and the relative standard deviation (R.S.D.) was below 6%.     

Benzyl vs. tropylium ions in the decomposition of some alkylnitrobenzenes

The [NO₂C₇H₆]⁺ ions generated from m-alkylnitrobenzenes have been shown to be different in their decomposition from those generated from p-alkylnitrobenzenes, even when the alkyl group is methyl and the departing fragment a hydrogen radical. Thus, in these cases even molecular ions of relatively high internal energy do not reversibly ring-expand to cycloheptatriene structures. In addition, the [NO₂C₇H₆]⁺ ions, assumed to be benzylic, do not ring-expand to nitrotropylium ions at internal energies sufficient to cause subsequent loss of NO or NO₂ from the p- and m-isomers, respectively.     

Hamilton cycles,avoiding prescribed arcs,in close-to-regular tournaments

The local irregularity of a digraph D is defined as i_l(D) = max {|d⁺ (x) − d⁻ (x)| : x ϵ V(D)}. Let T be a tournament, let Γ = {V₁, V₂, …, V_c} be a partition of V(T) such that |V₁| ≥ |V₂| ≥ … ≥ |V_c|, and let D be the multipartite tournament obtained by deleting all the arcs with both end points in the same set in Γ. We prove that, if |V(T)| ≥ max{2i_l(T) + 2|V₁| + 2|V₂| − 2, i_l(T) + 3|V₁| − 1}, then D is Hamiltonian. Furthermore, if T is regular (i.e., i_l(T) = 0), then we state slightly better lower bounds for |V(T)| such that we still can guarantee that D is Hamiltonian. Finally, we show that our results are best possible. © 1999 John Wiley & Sons, Inc. J Graph Theory 32: 123–136, 1999     

Solid phase synthesis of azapeptides using an automatic synthesizer

Peptides containing azaglycine located terminally or within the backbone have been prepared in the solid phase by means of an automatic synthesizer.     

On matching and total domination in graphs

A set M of edges of a graph G is a matching if no two edges in M are incident to the same vertex. A set S of vertices in G is a total dominating set of G if every vertex of G is adjacent to some vertex in S. The matching number is the maximum cardinality of a matching of G, while the total domination number of G is the minimum cardinality of a total dominating set of G. In this paper, we investigate the relationships between the matching and total domination number of a graph. We observe that the total domination number of every claw-free graph with minimum degree at least three is bounded above by its matching number, and we show that every k-regular graph with k?3 has total domination number at most its matching number. In general, we show that no minimum degree is sufficient to guarantee that the matching number and total domination number are comparable.