Rapid identification of unknown microorganisms of clinical and agricultural importance is not only critical for accurate diagnosis of infections but also essential for appropriate and prompt treatment. We describe here a rapid method for microorganisms typing based on quantitative analysis of fatty acids by iFAT approach (Isotope-coded Fatty Acid Transmethylation). In this work, lyophilized cell lysates were directly mixed with 0.5M NaOH solution in d3-methanol and n-hexane. After 1 min of ultrasonication, the top n-hexane layer was combined with a mixture of standard d0-methanol derived fatty acid methylesters with known concentration. Measurement of intensity ratios of d3/d0 labeled fragment ion and molecular ion pairs at the corresponding target fatty acids provides a quantitative basis for hierarchical clustering. In the resultant dendrogram, the Euclidean distance between unknown species and known species quantitatively reveals their differences or shared similarities in fatty acid related pathways. It is of particular interest to apply this method for typing fungal species because fungi has distinguished lipid biosynthetic pathways that have been targeted for lots of drugs or fungicides compared with bacteria and animals. The proposed method has no dependence on the availability of genome or proteome databases. Therefore, it is can be applicable for a broad range of unknown microorganisms or mutant species. 相似文献
In this paper, we consider a multi-dimensional system of thermoelasticity type III with a viscoelastic damping acting on a
part of the boundary. We establish a general decay result, from which the usual exponential and polynomial decay rates are
only special cases. 相似文献
In this paper, we extend some results of D.Dolzan on finite rings to profinite rings, a complete classification of profinite
commutative rings with a monothetic group of units is given. We also prove the metrizability of commutative profinite rings
with monothetic group of units and without nonzero Boolean ideals. Using a property of Mersenne numbers, we construct a family
of power 2ℵ0 commutative non-isomorphic profinite semiprimitive rings with monothetic group of units. 相似文献
This work is concerned with a system of two viscoelastic wave equations with nonlinear damping and source terms acting in
both equations. Under some restrictions on the nonlinearity of the damping and the source terms, we prove that, for certain
class of relaxation functions and for some restrictions on the initial data, the rate of decay of the total energy depends
on those of the relaxation functions. This result improves many results in the literature, such as the ones in Messaoudi and
Tatar (Appl. Anal. 87(3):247–263, 2008) and Liu (Nonlinear Anal. 71:2257–2267, 2009) in which only the exponential and polynomial decay rates are considered. 相似文献
In this paper, firstly, our purpose is to give the relationship among the densities of the sets of collinear points, the relationship among the densities of the sets of noncollinear points, and the relationship among the densities of the sets of the intersecting lines in Euclidean plane, respectively. In addition to that, we define the density formulas for the sets of points and lines under the two‐parameter planar Euclidean motion. By means of these results, we obtain essential properties that explain the connection among the densities of the sets of points and among the densities of the sets of intersecting lines under the two‐parameter planar Euclidean motion. 相似文献
We define curves on a Riemannian manifold as integrals of generalized Jacobi fields. We show that the force term that deviates the trajectory from the geodesic motion can be constructed as a functional of the metric tensor. These curves can be interpreted as particles (observers) coupled nonminimally with gravitation that can provide a class of residual observers for the inevitable singularity—as shown in the text.This essay received an honorable mention (1976) from the Gravity Research Foundation-Ed. 相似文献
Summary This paper deals with an elliptic boundary value problem posed in the plane, with variable coefficients, but whose restriction to the exterior of a bounded domain reduces to a Helmholtz equation. We consider a mixed variational formulation in a bounded domain that contains the heterogeneous medium, coupled with a boundary integral method applied to the Helmholtz equation in . We utilize suitable auxiliary problems, duality arguments, and Fredholm alternative to show that the resulting formulation of the problem is well posed. Then, we define a corresponding Galerkin scheme by using rotated Raviart-Thomas subspaces and spectral elements (on the interface). We show that the discrete problem is uniquely solvable and convergent and prove optimal error estimates. Finally we illustrate our analysis with some results from computational experiments. 相似文献
This contribution presents a new strategy for preparing nanocapsules with a shell made of a supramolecular polymer which repeating units are held together by reversible interactions rather than covalent bonds. These nanocapsules were prepared in classical miniemulsion through interfacial addition reaction of a diisocyanate (IPDI) and a monoamine (iBA), forming low‐molecular weight bis‐ureas moieties which are strong self‐complementary interacting molecules through hydrogen‐bonding. The nanocapsules present a diameter around 100 nm, and MALDI‐TOF MS and 1H NMR analyses confirm the expected molecular characteristics for the shell. This strategy opens the scope of a new type of nanomaterials exhibiting stimuli‐responsiveness due to the reversible interaction linking the repeating units.
In this paper, we consider the nonlinear viscoelastic equation
with initial conditions and Dirichlet boundary conditions. For nonincreasing positive functions g and for p>m, we prove that there are solutions with positive initial energy that blow up in finite time. 相似文献