Reversed‐Phase High‐Performance Liquid Chromatographic Separation and Determination of 4‐Amino‐azobenzene‐4′,5‐disulfonic Acid and Related Products in Industrial Wastewater Effluents

A simple and rapid reversed‐phase high‐performance liquid chromatographic method for the separation and determination of 4‐amino‐azobenzene‐4′,5‐disulfonic acid (AABDS) and its process‐related impurities was developed. The separation was achieved on a μ‐Bondapak C₁₈ column using 0.15 M ammonium sulfate‐acetonitrile (55:45) (v/v) as eluent. A UV‐visible spectrophotometric detector fixed at 386 nm was used both for detection and quantitation. The method was used not only for quality assurance but also for process development and wastewater management of AABDS.     

Impact of Gamma Irradiation and Kale Leaf Powder on Amino Acid and Fatty Acid Profiles of Chicken Meat under Different Storage Intervals

The present study was planned to determine the effect of kale leaf powder and gamma rays on variations in the pH, amino acid and fatty acid profiles of chicken meat at different storage intervals. Significant changes (p ≤ 0.05) in the pH, amino acid and fatty acid profiles of chicken meat following different treatments (KLP (1% and 2%) and gamma irradiation (3k Gy)) were reported at 0, 7 and 14 days of storage. The pH value of the chicken meat sample decreased with the addition of kale leaf powder, whereas the value increased following a gamma irradiation dose of 3 kGy and with the passage of time. During different storage intervals, the minimum reduction in the amino acid and fatty acid quantities in the chicken meat samples was reported after gamma irradiation treatment. However, with the addition of KLP, the amount of amino acids and fatty acids in the chicken meat samples increased. Conclusively, the pH was observed to be reduced in the meat following combined treatment (irradiation + KLP), whereas the 2% KLP treatment improved the amino acid and fatty acid profiles of the chicken samples.     

Oleuropein as a Potent Compound against Neurological Complications Linked with COVID-19: A Computational Biology Approach

The association of COVID-19 with neurological complications is a well-known fact, and researchers are endeavoring to investigate the mechanistic perspectives behind it. SARS-CoV-2 can bind to Toll-like receptor 4 (TLR-4) that would eventually lead to α-synuclein aggregation in neurons and stimulation of neurodegeneration pathways. Olive leaves have been reported as a promising phytotherapy or co-therapy against COVID-19, and oleuropein is one of the major active components of olive leaves. In the current study, oleuropein was investigated against SARS-CoV-2 target (main protease 3CL^pro), TLR-4 and Prolyl Oligopeptidases (POP), to explore oleuropein potency against the neurological complications associated with COVID-19. Docking experiments, docking validation, interaction analysis, and molecular dynamic simulation analysis were performed to provide insight into the binding pattern of oleuropein with the three target proteins. Interaction analysis revealed strong bonding between oleuropein and the active site amino acid residues of the target proteins. Results were further compared with positive control lopinavir (3CL^pro), resatorvid (TLR-4), and berberine (POP). Moreover, molecular dynamic simulation was performed using YASARA structure tool, and AMBER14 force field was applied to examine an 100 ns trajectory run. For each target protein-oleuropein complex, RMSD, RoG, and total potential energy were estimated, and 400 snapshots were obtained after each 250 ps. Docking analyses showed binding energy as −7.8, −8.3, and −8.5 kcal/mol for oleuropein-3CL^pro, oleuropein-TLR4, and oleuropein-POP interactions, respectively. Importantly, target protein-oleuropein complexes were stable during the 100 ns simulation run. However, an experimental in vitro study of the binding of oleuropein to the purified targets would be necessary to confirm the present study outcomes.     

On MHD transient flow of a Maxwell fluid in a porous medium and rotating frame

Analytic solution for unsteady magnetohydrodynamic (MHD) flow is constructed in a rotating non-Newtonian fluid through a porous medium. Constitutive equations for a Maxwell fluid have been taken into consideration. The hydromagnetic flow in the uniformly rotating fluid is generated by a suddenly moved infinite plate in its own plane. Analytic solution of the governing flow problem is obtained by means of the Fourier sine transform. It is shown that the obtained solution satisfies both the associate partial differential equation and the initial and boundary conditions. The solution for a Navier-Stokes fluid is recovered if λ→0. The steady state solution is also obtained for t→∞.     

Synthesis,characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Journal of Thermal Analysis and Calorimetry - This paper deals with an advanced colorimetric method used to determine the catalase mimetic activity of V2O5 nanoparticles by measuring the decrease...     

Prediction of low-sensitivity reactive dye recipe in exhaust dyeing influenced by material to liquor ratio and nature of salt

Reactive dyeings were carried out by exhaust method on 100% cotton knits. A trichromatic combination was chosen with only change in blue component. Colorimetric data were produced under controlled dyeing conditions by comparing the color difference between the target shade and resulting shades. Giving a change in liquor ratio and nature of salt the colorimetric data were regenerated again produced the shades. The data will be helpful to predict the low-sensitivity reactive dye recipe, which lead to the concept of right-first-time dyeing. The aim of this research is to help a dyer to select the right recipe. A set of the dye recipes was applied by dyeing with reactive dyes on cotton. Sodium chloride shows best results in terms of dye sensitivity as compared to Glauber's salt at low liquor ratio that is 1:10. Blue BRF in combination with yellow and red shows best result as compared to navy blue BF.     

A New Indole Alkaloid from Cleome droserifolia

The phytochemical investigations on Cleome droserifolia resulted in the isolation and characterization of a new indole alkaloid, 5‐hydroxy‐2‐methoxy‐1‐methyl‐1H‐indole‐3‐carbaldehyde ( 1 ). The structure elucidation was carried out on the basis of 1D‐ and 2D‐NMR techniques. In addition to 1 , two known aromatic derivatives, veratrol ( 2 ) and 2‐methoxy‐4‐methylacetophenone ( 3 ), were also obtained. All these compounds were purified by repeated column chromatography of the CH₂Cl₂ fraction obtained from MeOH extract of Cleome droserifolia. The structure of the new compound 1 was finally confirmed by the combined 1D (¹H‐ and ¹³C‐) and 2D (H? C correlations; HMBC and HSQC) NMR and IR spectroscopy, mass spectrometry (MS), and UV absorption spectroscopy techniques. The comparison of the physical and spectroscopic data with those in the literature provided evidence for the structure confirmation of known compounds. All the purified compounds were subjected to urease and α‐glucosidase enzymes inhibition. The results showed that compound 1 was more potent with an IC₅₀ value 11.97±2.067 μg/ml towards urease inhibition, while the activity of α‐glucosidase enzyme was marginal.     

Heat pipes: progress in thermal performance enhancement for microelectronics

Journal of Thermal Analysis and Calorimetry - The aim of this study is to oversee the impact of various techniques on thermal performance of heat pipes and to comprehensively cover the progress...