Development of new multifunctional terpolymer sorbent for proteomics applications

Determination of the availability of phases for specific separations is an important task achieved by a separation chemist. This becomes vital when the complex samples like biofluids are dealt with in proteome science. The work presented here involves the synthesis and application of terpolymeric sorbent with different functionalizations adopted for the selective enrichment of biomolecules of interest from biological fluids. Synthesis of terpolymer was carried out by the radical polymerization of monomers: methyl acrylate, acrylic acid and vinyl acetate with diethylene glycol dimethacrylate as cross‐linking agent, benzoyl peroxide as initiator and chloroform as a porogenic solvent. Characterization was done through Fourier transform infrared spectroscopy, scanning electron microscopy and nitrogen adsorption porosimetry. The polymer was further modified to immobilized metal ion affinity chromatographic material, with immobilized Fe³⁺/La³⁺ ions that allowed phosphopeptide enrichment from tryptic digests of standard proteins as well as milk, egg yolk and human serum. Sensitivity of enrichment down to 50 fmol was achieved in the presence of complex protein background as bovine serum albumin. Hydrophobicity was introduced through octadecyl amine, which provides comparable results to ZipTip C₁₈/C₄ for desalting of complex mixtures of all caseins. Analysis of the enriched content was performed by Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI‐MS). Copyright © 2014 John Wiley & Sons, Ltd.     

A Green Strategy to Prepare Warfarin‐like Compounds Catalyzed by Zirconium Oxychloride

A one‐pot, three‐component synthesis of warfarin‐like compounds from the reaction of benzamide, aryl glyoxal and 4‐hydroxycoumarin is described. Zirconium oxychloride octahydrate catalyzed the reactions efficiently under solvent‐free conditions to yield desired products in good yields.     

Application of a new equation of state to energy carriers

A new equation of state (IR EOS) recently reported for liquids and gases has been utilized to predict the densities of some energy carriers at different temperatures, pressures. The ability of IR EOS is examined by comparing its results with experimental data for some energy carriers in homogeneous gas, homogeneous liquid and gas–liquid transition region from low to very high pressures. The IR EOS gives excellent results in homogenous gas and homogeneous liquid region while its predictions in gas–liquid transition have more deviations. The average absolute deviation between calculated and experimental densities for 968 data points of 12 energy carriers is 0.33% over the entire range of data with a maximum pressure of 1000 MPa.     

UV-Visible Spectrophotometric Investigations of Charge Transfer Complexes of Some P-N,N-Dimethylaminobenzylidene Ailines with the New Acceptor PFB and CA Acceptor

The CT complexes of PFB and CA acceptors with eleven benzylidene anilines donors have been investigated spectro-photometrically in the uv-visible region for the first time. The values of Ip of these compounds have been estimated from their complexes with CA, the values are in the range of 8.64–8.96 ev. From these values the constants of the new acceptor PFB a, b, c₁, c₂ and Ea have been calculated, the values are 0.91, -5.31, 7.13, 1.97, and 1.37 ev, respectively. The CT parameters of our donors with PFB and CA acceptors, ζCT, ^KCT ΔG° and W have also been estimated and discussed.     

Total synthesis of achlisocoumarins I-II from Achlys triphylla

A concise and efficient total synthesis of achlisocoumarin I-II has been completed featuring a construction of the isocoumarin skeleton in a single step and the geranyl coupling with bromoarene ring at −10 °C exclusive of any chelating ligand.