Detection of Tumor Necrosis Factor Gene by Piezoelectric Nucleic Acid Senosr

A piezoelectric nucleic acid sensor was constructed ofr detection of tumor necrosis factor gene.Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal.The results show that polyethyleneimine adbesion and glutaraldehyde cross-linking method has higher sensitivity,stability and selectivity than protein A method.The solid-phase nucleic acid hybridization of oligo unclecotides and tumor necrosis factor target gene sequence were monitorde using this sensor.Tumor necrosis factor gene sequence(580bp) was detected by this nucleic acid sensor for the first time.     

Electrogenerated Chemiluminescence Based on Tris(2,2′-bipyridyl) Ruthenium(II) with Hydroxyl Carboxylic Acid

Upon the electrochemical oxidation of tris(2,2′-bipyridyl) ruthenium(II) [Ru(bpy)²⁺₃] and hydroxyl carboxylic acids, for instance, citric acid, tartaric acid, malic acid, and -gluconic acid, bright electrochemiluminescences (ECLs) were observed. Different luminescent reactions were presented depending on the applied potential. The light emission was mainly caused by the reaction between alkoxide radical ion and Ru(bpy)³⁺₃below the potential +1.80 V (vs Ag/AgCl). The luminescence intensity obviously increased because of the more complex reaction process. The luminescence wavelength of 608 nm, which could be found either at higher potential than +1.80 V or in the potential range from +1.30 to +1.80 V, confirmed that ECL was caused by Ru(bpy)²⁺₃*. The factors which affect the determination and HPLC separation of the four acids were also investigated.     

烯烃聚合数学模拟研究与现状

综述了近年来国内外在烯烃聚合微观形态和宏观反应器数学模拟方面取得的研究成果，指出了烯烃聚合过程数学模拟的发展方向。     

Reaction Ergodography and Transition State Analyses of the Addition of AIH_3 to Acetylene

The present paper covers the reaction ergodography for the addition of AlH3 to acetylene investigated by ab initio calculation with RHF/3-21G basis set. The changes in some physical properties along the reaction path (IRC) are presented. The formations of FMOs of the transition state have been analyzed according to the Fukui' s method. On the basis of the perturbation theory and Wolfsborg Helmholtz formula, we calculated the FMO' s energy orders of TS and its formation that shows the HOMO of TS consists of LUMO, HOMO of AlH3 and HOMO of HCCH.     

水杨酸与烟草铜锌超氧化物歧化酶的相互作用

利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸（SA）对超氧化物歧化酶（SOD）活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制，求得两者结合的形成常数和配位数，使用Forster非辐射能量转移技术求出了两者的结合位置距色氨酸残基间的距离为2.60nm。     

二氯亚甲基环己烷的卤化反应和2-取代二氯亚甲基环己烷的构象

多卤代烃在有机反应机理和结构研究中有着重要意义,作为一个有用的合成中间体,其制备也受到人们重视。我们在此报道二氯亚甲基环已烷(1)与氯及溴的反应和其它有关反应的实验结果。 1 于氯作用,主要得到加成产物1—氯—1—     

双核磺化酞菁铜(Ⅱ)催化氧化α-萘酚

本文研究了以乙醇为溶剂,CaCl_2为助催化剂,双核磺化酞菁铜(Ⅱ)催化氧化α-萘酚制备α-萘醌的各种因素。提出了可能的反应机理。     

Cell lysis on a microfluidic CD (compact disc)

Cell lysis was demonstrated on a microfluidic CD (Compact Disc) platform. In this purely mechanical lysis method, spherical particles (beads) in a lysis chamber microfabricated in a CD, cause disruption of mammalian (CHO-K1), bacterial (Escherichia coli), and yeast (Saccharomyces cerevisiae) cells. Interactions between beads and cells are generated in the rimming flow established inside a partially filled annular chamber in the CD rotating around a horizontal axis. To maximize bead-cell interactions in the lysis chamber, the CD was spun forward and backwards around this axis, using high acceleration for 5 to 7 min. Investigation on inter-particle forces (friction and collision) identified the following parameters; bead density, angular velocity, acceleration rate, and solid volume fraction as having the most significant contribution to cell lysis. Cell disruption efficiency was verified either through direct microscopic viewing or measurement of the DNA concentration after cell lysing. Lysis efficiency relative to a conventional lysis protocol was approximately 65%. In the long term, this work is geared towards CD based sample-to-answer nucleic acid analysis which will include cell lysis, DNA purification, DNA amplification, and DNA hybridization detection.     

The Luminescence Properties and Intramolecular Energy Transfer of Rare Earth Complexes with Aromatic Carboxylic Acids

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Oxodiperoxo molybdenum modified mesoporous MCM-41 materials for the catalytic epoxidation of cyclooctene

A hybrid heterogeneous catalyst system, which has been synthesized by covalently anchoring oxodiperoxo molybdenum chelate complexes onto the surface of mesoporous MCM-41, is highly active and truly heterogeneous for the liquid-phase epoxidation of cyclooctene with tBuOOH as the oxygen source.