体外培养三个人体肝癌细胞系的建立及其特征

从两例男性和一例女性肝癌患者获得的临床手术标本,经离体培养建成三系细胞,分别定名为:BEL-7402,BEL-7404和BEL-7405,在建系过程中(从第2代起),陆续对它们的生物学特征进行观察,细胞群体的倍增时间为20—26小时,动物异种移植能形成肿瘤结节,它们不仅在形态上均为上皮样细胞,且在电子显微镜下亦均显示上皮细胞所具有的桥粒和张力原纤维,它们的超显微结构显示与正常人肝细胞不同,而与临床肝癌细胞相似,三系细胞的染色体数均为不足三倍体值,均有一个异常长的近端着丝点染色体,但在BEL-7405系细胞中尚有微小的近端着丝点染色体和染色体断片的存在,免疫荧光间接法检测三系细胞内的AFP均显示阳性反应,LDH同功酶谱显示与成年人肝不同,而与人胚肝和临床肝癌相近似,即趋向于以LDH-1(H-型)比LDH-5(M-型)的酶活力高,上述几方面观察的结果,表明三系人肝癌均属生长迅速、分化较差的上皮样恶性细胞。     

千周和兆周范围的马氏体相变内耗以及预马氏体相变内耗

在千周和兆周两个频段分别测量了含Cd为46.1at％,47.5at％,50.0at％和52.5at％的AuCd合金中与马氏体转变有关的内耗和杨氏模量。实验结果表明,在千周范围,由于马氏体转变引起的Q^-1(内耗)是静滞后型的。Q^-1峰的形成可以用弹性软化及界面位错的静滞后阻尼机制来解释。而兆周范围的超声衰减是与频率有关的,表明尚有共振型及弛豫型阻尼的成份。此外,实验还观察到AuCd合金的等温马氏体转变Q^-1峰以及预马氏体相变Q^-1峰。 关键词：     

DEVELOPMENT AND IDENTIFICATION OF CHINESE SPRING Ta1 kr ph1b PLANTS AND THEIR USE ON GENETIC TRANSFER OF DESIRABLE GENES FROM Aegilops

Chinese Spring Tal kr phlb plants were developed by hybridizing Tal common wheat and Chinese Spring phlb mutant and backcrosses. When Chinese Spring Tal kr phlb plants were crossed with some wild species of Triticeae, much more seeds of wide hybridization can be obtained without artificial emasculation and pollination because of the existence of Tat and kr genes. The homoeologous chromosomes between common wheat and the wild species of Triticeae can also pair and exhibit cross-over in the hybrid F1 plants because of the existence of the phlb gene. So that more alien desirable genes can be transferred to common wheat rapidly. Therefore, Chinese Spring Tal kr phlb plants become a powerful tool for wide hybridization and transferring alien desirable genes to common wheat.     

GaAs/AlGaAs异质结中二维电子气子能带的Landau能级耦合

采用栅压比谱技术，在相对较低的磁场下，观察到GaAs/AlGaAs异质结中二维电子气第零子能带的N＝1 Landau能级与高达第五子能带的N＝0 Landau能级的共振耦合现象，由此精确测量了各子能带能量间距，并与自洽的理论计算结果相比较.同时，讨论了共振子能带-Landau能级耦合所引起的Landau能级分裂大小与子能带量子数的关系.     

FORMATION AND MAGNETIC PROPERTIES OF Dy2Fe17-xMnxC(x = 0-17) PREPARED BY ARC-MELTING

We report on alloys formed by replacing iron with manganese in the compound Dy₂Fe₁₇C_1.0 Samples were characterized by X-ray diffraction and magnetic measure-ments, The 2:17-type structure can be crystallized in the whole range of manganese substitution. The Curie temperature of the series of Dy₂Fe_17-xMn_xC_1.0(x= 0-17) drops down considerably from 515K for x = 0 to about 20K for compounds with high manganese concentration, and the compensation point was observed in a narrow range of x≈4-6. The rapid decrease of the magnetization shows that the manganese substitution is not a simple magnetic dilution to the transition-metal sublattice mo-ment, it indicates that the moment of Mn may be antiparallel to that of Fe. The field dependence of the magnetization of Dy₂Fe_17-xMn_xC_1.0 may indicate the existence of the nonlinear magnetic structure for the samples with high Mn conoentrations.     

倒浮萍聚合物LB膜的有序－无序相变研究

倒浮萍聚合物ＬＢ膜的有序－无序相变研究李宏斌，张希，张瑞丰，赵冰，徐尉青，沈家骢，高忠民（吉林大学分子光谱与分子结构开放实验室、化学系，分析测试中心，长春，１３００２３）关键词聚合物ＬＢ膜，有序－无序相变，变温红外光谱，变温小角Ｘ光衍射ＬＢ膜的有序－...     

Adsorption of Benzene on the Potassium Exchanged Hbeta Zeolites

ＡｄｓｏｒｐｔｉｏｎｏｆＢｅｎｚｅｎｅｏｎｔｈｅＰｏｔａｓｓｉｕｍＥｘｃｈａｎｇｅｄＨｂｅｔａＺｅｏｌｉｔｅｓＳＨＥＮＪｉａｎ－ｐｉｎｇ；ＭＡＪｕｎ；ＳＵＮＴｉｅａｎｄＪＩＡＮＧＤａ－ｚｈｅｎ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＪｉｌｉｎＵ...     

两级非磁性超高压装置

本文首次报道一种用于高压下电磁现象研究的两级非磁性超高压装置,描述了其结构原理和设计概要。这一装置目前达到的最高调试压力为28kbar,这是完全流体静力学的压力。利用本装置,已在0—25kbar的压力范围内进行了锑化铟、锗、碲镉汞等的电阻率和霍耳系数随压力变化的测量,对于这些材料能带结构和迁移率特性随压力变化,已获得令人满意的结果。 关键词：     

白光LED用Ca_8Mg(SiO_4)_4Cl_2:Eu~(2+),Dy~(3+)发光粉的发光性能

采用高温固相法合成了白光LED用Ca8Mg(SiO4)4Cl2:Eu和Ca8Mg(SiO4)4Cl2:Eu,Dy绿色发光粉。研究发现:共掺Dy可以明显地提高Ca8Mg(SiO4)4Cl2:Eu发光粉的发光性能,表明Dy3+和Eu2+之间存在着能量传递过程。当Dy3+的最佳掺杂摩尔分数为0.02时,发光粉505nm处绿光发射的强度约提高12%。通过对Dy3+和Eu2+光谱特性的分析,Dy3+和Eu2+之间的能量传递机制可归因于无辐射交叉弛豫。     

虎皮楠中2个黄酮苷的波谱学研究

研究了虎皮楠中的抗氧化活性成分.应用D101大孔吸附树脂、Sephadex LH-20及制备型高效液相色谱进行分离,应用波谱学(UV,ESI-MS,1H NMR,13C NMR,1H-1H COSY,HSQC,HMBC等)方法进行结构鉴定.分离得到1个二氢黄酮苷类化合物和1个黄酮苷类化合物,确定了1H NMR,13C NMR信号的全归属,化合物1鉴定为5,4′-二羟基二氢黄酮-7-O-α-L-鼠李糖(1→2)-β-D-葡萄糖苷,化合物2鉴定为7,4′-二羟基异黄酮-8-C-β-D-葡萄糖碳苷.