Duty cycle and directional jet effects of a plasma actuator on the flow control around a NACA0015 airfoil

This paper reports on the effects of a series of fluid-dynamic dielectric barrier discharge plasma actuators on a NACA0015 airfoil at high angle of attack. A set of jet actuators able to produce plasma jets with different directions (vectoring effect) and operated at different on/off duty cycle frequencies are used. The experiments are performed in a wind tunnel facility. The vectorized jet and the transient of the flow induced by unsteady duty cycle operation of each actuator are examined and the effectiveness of the actuator to recover stall condition in the range of Reynolds numbers between 1.0 × 10⁵ and 5.0 × 10⁵ (based on airfoil chord), is investigated. The actuator placed on the leading edge of the airfoil presents the most effective stall recovery. No significant effects can be observed for different orientations of the jet. An increase of the stall recovery is detected when the actuator is operated in unsteady operation mode. Moreover, the frequency of the on/off duty cycle that maximizes the stall recovery is found to be a function of the free stream velocity. This frequency seems to scale with the boundary layer thickness at the position of the actuator. A lift coefficient increase at low free stream velocities appears to linearly depend on the supply voltage.     

Polarization dependence of electroluminescence from closely-stacked and columnar quantum dots

Quantum dots (QDs) have a potential for application in semiconductor optical amplifiers (SOAs), due to their high saturation power related to the low differential gain, fast gain recovery and wide gain spectrum compared to quantum wells. Besides all advantages, QDs realized by Stranski-Krastanov growth mode have a flat shape which leads to a gain anisotropy and a related transverse magnetic (TM) and -electric (TE) polarization dependence as compared to bulk material. This has so far prevented their applications in SOAs. It has been suggested that control of optical polarization anisotropy of the QD can be obtained through QD shape engineering, in closely stacked or columnar QDs (CQDs). To this aim, we have fabricated and tested SOA structures based on closely-stacked and columnar QDs. Closely-stacked InAs QDs with 4, 6 and 10 nm GaAs spacer showed a minor improvement in the ratio of TM and TE integrated electroluminescence (EL) over standard QDs along with a strong reduction in efficiency. In contrast, a large improvement was obtained in CQDs, depending on the number of stacked submonolayers which can be attributed to the more symmetric shape of columnar QDs. A relatively small spectral separation (ΔE ~ 21 meV) between TE- and TM-EL peaks has been observed showing that heavy- and light hole-like states, respectively are energetically close in these QDs. These results indicate that columnar QDs have a significant potential for polarization-independent QD SOA.     

Comparison of Twisted P-Form Spectra for Flat Manifolds with Diagonal Holonomy

We give an explicit formula for the multiplicities of the eigenvalues ofthe Laplacian acting on sections of natural vector bundles over acompact flat Riemannian manifold M = \ ⁿ , a Bieberbach group. In the case of the Laplacian acting onp-forms, twisted by a unitary character of , when hasdiagonal holonomy group F ₂ ^k , these multiplicities have acombinatorial expression in terms of integral values of Krawtchoukpolynomials and the so called Sunada numbers. If the Krawtchoukpolynomial K _p ⁿ (x)does not have an integral root, this expressioncan be inverted and conversely, the presence of such roots allows toproduce many examples of p-isospectral manifolds that are notisospectral on functions. We compare the notions of twistedp-isospectrality, twisted Sunada isospectrality and twisted finitep-isospectrality, a condition having to do with a finite part of thespectrum, proving several implications among them and getting a converseto Sunada's theorem in our context, for n 8. Furthermore, a finitepart of the spectrum determines the full spectrum. We give new pairs ofnonhomeomorphic flat manifolds satisfying some kind of isospectralityand not another. For instance: (a) manifolds which are isospectral onp-forms for only a few values of p 0, (b) manifolds which aretwisted isospectral for every , a nontrivial character of F, and(c) large twisted isospectral sets.     

Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler

A capillary electrophoresis apparatus was used as sampler for flow injection analysis (FIA) in tandem mass spectrometry of L-carnitine and its acetyl- and propionyl-metabolites in human plasma. The capillary electrophoresis instrument was coupled to the ion trap mass spectrometer by an electrospray ionization coaxial sheath liquid interface. The electrophoresis capillary introduced the sample directly into the source by applying a prolonged sample injection. The use of the capillary electrophoresis apparatus miniaturised the FIA procedure, substantially reducing the quantities of solvents and samples used, and allowed rapid automated sequential analyses. The method was optimised and validated using a dialyzed human plasma matrix. The plasma samples were analysed after a simple, rapid deproteinisation procedure with acetonitrile and diluted 70 times before direct injection into the mass spectrometer for product ion scan MS/MS analysis in positive ionisation. The total analysis time was 5 min, including capillary preconditioning and acquisition time (3 min). The method was sensitive, allowing the determination of L-, L-acetyl- and L-propionyl-carnitines at 140, 14 and 3.6 nM concentrations (injected values) corresponding to lower limit of quantitation values in plasma of 10, 1 and 0.25 microM, respectively. The method was processed for full validation and applied to the analysis of L-carnitine and its short chain derivatives in human plasma samples.     

Asymmetric distribution of phosphatidyl serine in supported phospholipid bilayers on titanium dioxide

Supported phospholipid bilayers (SPBs) are useful for studying cell adhesion, cell-cell interactions, protein-lipid interactions, protein crystallization, and applications in biosensor and biomaterial areas. We have recently reported that SPBs could be formed on titanium dioxide, an important biomaterial, from vesicles containing anionic phospholipid phosphatidyl serine (PS) in the presence of calcium. Here, we show that the mobility of the fluorescently labeled PS present in these bilayers is severely restricted, whereas that of the zwitterionic phosphatidyl choline is not affected. Removal of calcium alleviated the restriction on the mobility of PS. Both components were found to be mobile in SPBs of identical compositions prepared in the presence of calcium on silica. To explain these results, we propose that, on TiO2, PS is trapped in the proximal leaflet of the bilayers. This proposal is supported by the results of protein adsorption experiments carried out on bilayers containing various amounts of PS prepared on silica and titania.     

The Interplay of Cholesterol and Ligand Binding in hTSPO from Classical Molecular Dynamics Simulations

The translocator protein (TSPO) is a 18kDa transmembrane protein, ubiquitously present in human mitochondria. It is overexpressed in tumor cells and at the sites of neuroinflammation, thus representing an important biomarker, as well as a promising drug target. In mammalian TSPO, there are cholesterol–binding motifs, as well as a binding cavity able to accommodate different chemical compounds. Given the lack of structural information for the human protein, we built a model of human (h) TSPO in the apo state and in complex with PK11195, a molecule routinely used in positron emission tomography (PET) for imaging of neuroinflammatory sites. To better understand the interactions of PK11195 and cholesterol with this pharmacologically relevant protein, we ran molecular dynamics simulations of the apo and holo proteins embedded in a model membrane. We found that: (i) PK11195 stabilizes hTSPO structural fold; (ii) PK11195 might enter in the binding site through transmembrane helices I and II of hTSPO; (iii) PK11195 reduces the frequency of cholesterol binding to the lower, N–terminal part of hTSPO in the inner membrane leaflet, while this impact is less pronounced for the upper, C–terminal part in the outer membrane leaflet, where the ligand binding site is located; (iv) very interestingly, cholesterol most frequently binds simultaneously to the so-called CRAC and CARC regions in TM V in the free form (residues L150–X–Y152–X(3)–R156 and R135–X(2)–Y138–X(2)–L141, respectively). However, when the protein is in complex with PK11195, cholesterol binds equally frequently to the CRAC–resembling motif that we observed in TM I (residues L17–X(2)–F20–X(3)–R24) and to CRAC in TM V. We expect that the CRAC–like motif in TM I will be of interest in future experimental investigations. Thus, our MD simulations provide insight into the structural features of hTSPO and the previously unknown interplay between PK11195 and cholesterol interactions with this pharmacologically relevant protein.     

Harnessing Effective Molarity to Design an Electrochemical DNA-based Platform for Clinically Relevant Antibody Detection

Easy-to-use platforms for rapid antibody detection are likely to improve molecular diagnostics and immunotherapy monitoring. However, current technologies require multi-step, time-consuming procedures that limit their applicability in these fields. Herein, we demonstrate effective molarity-driven electrochemical DNA-based detection of target antibodies. We show a highly selective, signal-on DNA-based sensor that takes advantage of antibody-binding-induced increase of local concentration to detect clinically relevant antibodies in blood serum. The sensing platform is modular, rapid, and versatile and allows the detection of both IgG and IgE antibodies. We also demonstrate the possible use of this strategy for the monitoring of therapeutic monoclonal antibodies in body fluids. Our approach highlights the potential of harnessing effective molarity for the design of electrochemical sensing strategies.     

Modeling of gain and phase dynamics in quantum dot amplifiers

By means of an electron hole rate equation model we explain the phase dynamics of a quantum dot semiconductor optical amplifier and the appearance of different decay times observed in pump and probe experiments. The ultrafast hole relaxation leads to a first ultrafast recovery of the gain, followed by electron relaxation and, in the nanosecond timescale, radiative and non-radiative recombinations. The phase dynamics is slower and is affected by thermal redistribution of carriers within the dot. We explain the ultrafast response of quantum dot amplifiers as an effect of hole escape and recombination without the need to assume Auger processes.     

Laser-induced ablation and crater formation at high laser flux

Laser ablation and crater formation have been studied on a copper target using a 10 Hz Nd:YAG laser system delivering pulses up to 100 mJ in 40 ps with a flux on target F?≤?5000 J/cm². Crater dimensions were measured using optical microscope or scanning electron microscope. In order to understand the process of crater formation, we considered various theoretical models present in the literature and revised them taking into account the occurrence of plasma phenomena, which are important at the intensities used in this experiment. We also compared our experimental results with other results obtained at the PALS laboratory, using a 0.44 μm wavelength laser and much higher laser intensities. Finally, we explore the possibility of extending the information derived from laser-produced craters to other types of craters.