Photooxidation of lens proteins with xanthurenic acid: a putative chromophore for cataractogenesis.

The tryptophan metabolite, xanthurenic acid (Xan), is produced through a transamination reaction in high concentrations in human lenses with age and has been isolated from aged human cataractous lenses. It has appreciable absorption between 300 and 400 nm (lambda max = 334 nm), the range absorbed by the human lens. Our recent studies have shown that unlike most tryptophan metabolites in the eye, Xan is photochemically active, producing both superoxide and singlet oxygen. To determine if Xan could act as a photosensitizer and photooxidize cytosolic lens proteins, alpha-, beta- and gamma-crystallins were irradiated (lambda > 300 nm, 12 mW/cm2) in the presence and absence of Xan. Upon irradiation and in the presence of Xan, lens proteins polymerized in the order alpha > beta > gamma as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Further analysis of the photolyzed alpha-crystallin by mass spectrometry indicated that histidine, tryptophan and methionine residues were oxidized at specific positions in a dose-dependent (irradiation time) manner. In alpha A-crystallin two forms of oxidized histidine 154 were observed, 2-imidazolone and 2-oxohistidine. Our results suggest that naturally occurring Xan is a chromophore capable of photosensitization and photooxidation of lens proteins. Furthermore, this compound could play a role in age-related cataractogenesis.     

The Positivity of Intersection Multiplicities and Symbolic Powers of Prime Ideals

Serre's nonnegativity conjecture for intersection multiplicities has recently been proven by O. Gabber. In this paper we investigate Serre's positivity conjecture using the methods which he developed. We show in particular that the positivity conjecture has implications for properties of symbolic powers of prime ideals in regular local rings.     

Simultaneous DPTV/PLIF measurements of a turbulent jet

Simultaneous measurements of instantaneous velocity and concentration fields were performed using digital particle tracking velocimetry and planar laser- induced fluorescence for a turbulent jet at a Reynolds number of 3000. The measurements of mean velocity, turbulent stresses, mean concentration, concentration variance, and turbulent flux of tracer all collapse onto self-similar profiles in the far field of the jet. The measurements showed excellent agreement with previous point velocity and concentration measurements. It is concluded that the system is an effective means of measuring the velocity and concentration distributions and turbulent characteristics. Received: 7 July 1999/Accepted: 9 February 2000     

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A flow-injection system for assay of the activity of an immobilized enzyme chemically-modified electrode

Chymotrypsin, covalently immobilized to the surface of an IrO₂-coated titanium electrode, responds potentiometrically to various substrates. A flow-injection system is described for assay of the activity of the immobilized enzyme with N-benzoyl-l-tyrosine ethyl ester as substrate and an ultraviolet detector. Least-squares fits of peak height vs. time typically yield correlation coefficients of 0.999 and standard errors of estimate of 0.0043 absorbance for a total absorbance change of about 0.130. Slopes of such plots vary linearly with enzyme activity.     

The influence of extraneous sounds on the perceptual estimation of first-formant frequency in vowels

The contribution of extraneous sounds to the perceptual estimation of the first-formant (F1) frequency of voiced vowels was investigated using a continuum of vowels perceived as changing from/I/to/epsilon/as F1 was increased. Any phonetic effects of adding extraneous sounds were measured as a change in the position of the phoneme boundary on the continuum. Experiments 1-5 demonstrated that a pair of extraneous tones, mistuned from harmonic values of the fundamental frequency of the vowel, could influence perceived vowel quality when added in the F1 region. Perceived F1 frequency was lowered when the tones were added on the lower skirt of F1, and raised when they were added on the upper skirt. Experiments 6 and 7 demonstrated that adding a narrow-band noise in the F1 region could produce a similar pattern of boundary shifts, despite the differences in temporal properties and timbre between a noise band and a voiced vowel. The data are interpreted using the concept of the harmonic sieve [Duifhuis et al., J. Acoust. Soc. Am. 71, 1568-1580 (1982)]. The results imply a partial failure of the harmonic sieve to exclude extraneous sounds from the perceptual estimation of F1 frequency. Implications for the nature of the hypothetical harmonic sieve are discussed.