Capillary electrophoretic detection in apolipoprotein E genotyping

We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20-100 base pairs. Especially discrimination between apoE2/E2 and apoE2/E3 genotypes is more reliable with CE than with AGE, this being of great clinical value in the diagnosis of familiary dysbetalipoproteinemia.     

Fully aromatic thermotropic liquid crystalline polyesters of substituted 4,4′-biphenols. IV. Homopolyesters with terephthalic acid and copolyesters with terephthalic acid and 4-hydroxybenzoic acid

A series of fully aromatic thermotropic polyesters based on mono-, di-, and tetra-substituted biphenols was prepared by the melt polycondensation method and examined for their thermotropic behavior by a variety of experimental techniques. The homopolyesters obtained from substituted biphenols containing either one phenyl or two phenyl groups as substituent(s) and TA formed nematic melts, but the homopolymers of the substituted biphenols containing either four sec-butyl groups or two tert-butyl groups with TA had melting transitions, T_m, above 400°C. Thus, it was not possible to determine whether they formed nematic melts. On copolymerization with 30 mol % HBA most of the resulting copolyesters had much lower T_m values, compared to those of respective homopolyesters, and the copolymers of the biphenol monomer containing the tert-butyl groups formed a nematic melt at an observable temperature. However, the copolymer of the biphenol with sec-butyl groups still had a T_m above 400°C. © 1993 John Wiley & Sons, Inc.     

Allylic vs homoallylic control of stereospecificity in the epoxidation of 3(1′-hydroxyethyl)-5,8-dimethoxy-1,2-dihydronaphthalen-1-ol: implications for the synthesis of chiral anthracyclines

The stereochemistry of the epcxidation of the title compound with t-BuOOH catalysed by VO(acac)₂ is subject to exclusive homoallylic control. Secondary allylic alcohols in the side chain regain their normal controlling influence over diastereoselection only when the homoallylic group is blocked.     

Synthesis and X-Ray crystal structure of a 2,4,5-trisubstituted 1,2,4-triazolin-3-one

The synthesis and x-ray crystal structure of BMY 13754, a 1,2,4-triazolin-3-one under clinical evaluation as an antidepressant agent, is described. Several synthetic strategies are discussed.     

Synthesis and structural characterisation of [(Ph3P)AuCo(CO)3(PPh3)]

Summary [(Ph₃P)AuCo(CO)₃(PPh₃)] has been synthesised from [(Ph₃P)AuCo(CO)₄], PPh₃v and Me₃NO in acetonitrile. Its molecular structure, determined by single-crystal x-ray crystallography, consists of an almost linear P-Au-Co-P arrangement in which the Co atom is in a slightly distorted trigonalbipyramidal geometry, with the Au and P atoms occupying the apical sites. The Au-Co bond length of 2.450(1) ? is shorter than that reported for [(Ph₃P)AuCo(CO)₄]. The carbonyl ligands are bent towards the Au atom and the mean Au-Co-C angle is 81(1)°.     

Strongly Phosphorescent Iridium(III)-Porphyrins - New Oxygen Indicators with Tuneable Photophysical Properties and Functionalities

Synthesis and characterization of four iridium(III)-octaethylporphyrins and a π-extended iridium(III)-benzoporphyrin are presented. Strong room-temperature phosphorescence was observed for all of the complexes with quantum yields of up to 30 %. Axial ligands were introduced to tune the photophysical properties and the solubility. Complexes bearing lipophilic ligands such as pyridine or N-(n-butyl)imidazole were incorporated into polystyrene to obtain optical oxygen sensors. Covalent coupling of the dye is possible by introduction of ligands with binding domains (1-imidazoleacetic acid). This enabled preparation of a water-soluble oxygen probe (by staining bovine serum albumin) and a trace oxygen sensor (by coupling to amino-modified silica gel).     

Synthesis and structure‐activity relationship of several aromatic ketone‐based two‐photon initiators

Several novel aromatic ketone‐based two‐photon initiators containing triple bonds and dialkylamino groups were synthesized and the structure‐activity relationships were evaluated. Branched alkyl chains were used at the terminal donor groups to improve the solubility in the multifunctional monomers. Because of the long conjugation length and good coplanarity, the evaluated initiators showed large two‐photon cross section values, while their fluorescence lifetimes and quantum yields strongly depend on the solvent polarity. All novel initiators exhibited high activity in terms of two‐photon‐induced microfabrication. This is especially true for fluorenone‐based derivatives, which displayed much broader processing windows than well‐known highly active initiators from the literature and commercially available initiators. While the new photoinitiators gave high reactivity in two‐photon‐induced photopolymerization at concentration as low as 0.1% wt, these compounds are surprisingly stable under one photon condition and nearly no photo initiation activity was found in classical photo DSC experiment. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011