Vanadin-Bestimmungsmethoden

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Gasanalyse

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Synthèse rationelle de clusters mixtes à trois au quatre métaux differents et contenant le ligand Ph2PCH2PPh2 (dppm)

Reaction of PdPtCl₂(dppm)₂ with Co(CO)₄^? or Mn(CO)₅^? affords the tetrametallic clusters PdPtCo₂(CO)₇(dppm)₂ and PdPtMn₂(CO)₉(dppm)₂. The cobalt cluster reacts with iodide to give PdPtCoI(CO)₃(dppm)₂ which upon reaction with Fe(CO)₃NO^? or Mn(CO)₅^? yields PdPtFeCo(CO)₆NO(dppm)₂ and PdPtMnCo(CO)₈(dppm)₂, respectively.     

Optically active transition metal compounds : LXXXI. Crystal structure of (+)546-[C5H5Mo(CO)2LL]PF (LL = 2-benzoylpyridine-(R)-1-phenylethylimine)

Racemic [C₅H₅Mo(CO)₂LL]PF₆, (2) with LL = 2-benzoylpyridine-1-phenylethylimine, undergoes spontaneous resolution upon crystallization from acetone/CH₂Cl₂/ethanol. The absolute configuration of the (+)₅₄₆-isomer was shown to be (R) at the Mo atom and (R) at the asymmetric carbon atom. Comparison of 2 with [C₅H₅Mo(CO)₂LL]PF₆ (1) (LL = 2-carbaldehydepyridine-1-phenylethylimine) reveals distinct changes caused by the differences resulting from the presence of the phenyl group in 2 and the change from the (RR)- to the (RS)-configuration.     

Site‐Specific Labeling of Affimers for DNA‐PAINT Microscopy

Optical super‐resolution techniques allow fluorescence imaging below the classical diffraction limit of light. From a technology standpoint, recent methods are approaching molecular‐scale spatial resolution. However, this remarkable achievement is not easily translated to imaging of cellular components, since current labeling approaches are limited by either large label sizes (antibodies) or the sparse availability of small and efficient binders (nanobodies, aptamers, genetically‐encoded tags). In this work, we combined recently developed Affimer reagents with site‐specific DNA modification for high‐efficiency labeling and imaging using DNA‐PAINT. We assayed our approach using an actin Affimer. The small DNA‐conjugated affinity binders could provide a solution for efficient multitarget super‐resolution imaging in the future.