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391.
Vetromile CM Perman J Cheney ML Zaworotko MJ Larsen RW 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,78(2):648-652
In this report the photo-physical properties of 9-amino acridine (9AA) associated with αZr-phosphate particles (αZrP) is examined. In ethanol solution 9AA exhibits absorption maxima at 425 nm, 402 nm and 383 nm as well as emission bands centered at 455 nm and 483 nm (using 423 nm excitation). The corresponding emission decay is monophasic with a lifetime of 16.5 ns. When αZrP is sonicated in the presence of an ethanol solution of 1 mM 9AA the resulting material exhibits a broad absorption band centered at ~400 nm with shoulders at ~425 nm and ~385 nm and emission bands at 462 nm and 485nm (using 423 nm excitation). Interestingly, the emission decay is biphasic with lifetimes of 1.6 ns and 9.8 ns constituting 57% and 43% of the total emission intensity, respectively. The absence of any shifts in the low angle XRD data suggests that 9AA associates via adsorption onto the exterior surfaces of the αZrP particles. Overall, these results are consistent with different modes of 9AA association to αZrP reflecting different degrees of H-bonding which significantly influences the extent of non-radiative decay from the lowest excited singlet state of 9AA. 相似文献
392.
For bottom-up MS, the digestion step is critical and is typically performed with trypsin. Solvent-assisted digestion in 80% acetonitrile has previously been shown to improve protein sequence coverage at shorter digestion times. This has been attributed to enhanced enzyme digestion efficiency in this solvent. However, our results demonstrate that tryptic digestion in 80% acetonitrile is less efficient than that of conventional (aqueous) digestion. This is a consequence of decreased enzyme activity beyond ∼40% acetonitrile, increased enzyme autolysis and lower protein solubility in 80% acetonitrile. We observe multiple missed cleavages and reduced concentration of fully cleaved digestion products. Nonetheless we confirm, through room temperature solvent-assisted digestion, a consistent improvement in protein sequence coverage when analyzed by mass spectrometry. These results are explained through the increased number of unique digestion products available for detection. Thus, while solvent-assisted digestion has clear merits for proteome analysis, one should be aware of the inefficiency of protein digestion though this protocol, particularly with absolute protein quantitation experiments. 相似文献
393.
Production of New Cladosporin Analogues by Reconstitution of the Polyketide Synthases Responsible for the Biosynthesis of this Antimalarial Agent 下载免费PDF全文
Rachel V. K. Cochrane Randy Sanichar Gareth R. Lambkin Béla Reiz Dr. Wei Xu Prof. Yi Tang Prof. John C. Vederas 《Angewandte Chemie (International ed. in English)》2016,55(2):664-668
The antimalarial agent cladosporin is a nanomolar inhibitor of the Plasmodium falciparum lysyl‐tRNA synthetase, and exhibits activity against both blood‐ and liver‐stage infection. Cladosporin can be isolated from the fungus Cladosporium cladosporioides, where it is biosynthesized by a highly reducing (HR) and a non‐reducing (NR) iterative type I polyketide synthase (PKS) pair. Genome sequencing of the host organism and subsequent heterologous expression of these enzymes in Saccharomyces cerevisiae produced cladosporin, confirming the identity of the putative gene cluster. Incorporation of a pentaketide intermediate analogue indicated a 5+3 assembly by the HR PKS Cla2 and the NR PKS Cla3 during cladosporin biosynthesis. Advanced‐intermediate analogues were synthesized and incorporated by Cla3 to furnish new cladosporin analogues. A putative lysyl‐tRNA synthetase resistance gene was identified in the cladosporin gene cluster. Analysis of the active site emphasizes key structural features thought to be important in resistance to cladosporin. 相似文献
394.
Elles CG Shkrob IA Crowell RA Arms DA Landahl EC 《The Journal of chemical physics》2008,128(6):061102
Time-resolved x-ray absorption spectroscopy has been used to observe the transient species generated by one-photon detachment of an electron from aqueous bromide. The K-edge spectrum of the short-lived Br(0) atom exhibits a resonant 1s-4p transition that is absent for the Br(-) precursor. The strong 1s-4p resonance suggests that there is very little charge transfer from the solvent to the open-shell atom, whereas weak oscillations above the absorption edge indicate that the solvent shell around a neutral Br(0) atom is defined primarily by hydrophobic interactions. These conclusions are in agreement with Monte Carlo and quantum chemical simulations of the solvent structure. 相似文献
395.
A reproducible analytical method for determination of nitrocellulose in soil is described. The new method provides the precision and accuracy needed for quantitation of nitrocellulose in soils to enable worker safety on contaminated sites. The method utilizes water and ethanol washes to remove co-contaminants, acetone extraction of nitrocellulose, and base hydrolysis of the extract to reduce nitrate groups. The hydrolysate is then neutralized and analyzed by ion chromatography for determination of free nitrate and nitrite. A variety of bases for hydrolysis and acids for neutralization were evaluated, with 5N sodium hydroxide and carbon dioxide giving the most complete hydrolysis and interference-free neutralization, respectively. The concentration of nitrocellulose in the soil is calculated from the concentrations of nitrate and nitrite and the weight percentage of nitrogen content in nitrocellulose. The laboratory detection limit for the analysis is 10mg/kg. The method acceptance range for recovery of nitrocellulose from control samples is 78-105%. 相似文献
396.
397.
More than eighty scientists from over ten different countries in Europe and America descended on Diamond Light Source in Oxfordshire to participate in the International Workshop on X-ray Spectroscopy of Magnetic Solids on June 10–21, 2010. The XRMS meetings have demonstrated to be a fruitful forum for informal discussion of recent results and future projects of synchrotron-radiation-based research on magnetism and also serve for the formation of new collaborations. The meetings are organized in sessions dedicated to specific scientific topics and experimental techniques. The absence of parallel sessions allows all the participants to take part in the discussion and to have a comprehensive view of the latest achievements and of the envisioned developments in the field. The previous meeting in the series was held last year at the synchrotron Soleil in St Aubin near Paris. The emerging new opportunities for magnetic spectroscopy, such as offered by the beamline for advanced dichroism experiments (BLADE), with its three intertwined assets of polarization, coherence, and time resolution, made it timely for the international community to convene at Diamond. 相似文献
398.
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400.
Randy M. Whittal Liang Li Sam Lee Mitchell A. Winnik 《Macromolecular rapid communications》1996,17(1):59-64
We report the characterization of a sample of poly(ethylene glycol) (PEG, M n = 3841, M w/M n = 1.01), and its derivative end-labeled with pyrenebutyrate groups, using high resolution MALDI time-of-flight mass spectrometry. A matrix of 2-(4-hydroxyphenyl-azo)benzoic acid containing a trace of either sodium chloride or potassium chloride was employed for laser desorption. Peaks due to the sodium or potassium cationized polymers were obtained, equally spaced at 44 mass units apart. For the pyrenebutyrate diester, the analysis shows that 80 ± 2% of the chains were doubly labeled, with the ramaining chains containing only a single pyrene group. Molecular weight determinations for both sets of samples were entirely consistent with size-exclusion chromatography measurements, but were obtained with greater accuracy and less ambiguity about the influence of the end groups on the hydrodynamic radius of the polymer. 相似文献