Interaction of antigen and antibody on core-shell polymeric microspheres

Monodispersed microspheres with polystyrene as the core and poly(acrylamide-co-N-acryloxysuccinirnide) as the shell were synthesized by a two-step surfactant-free emulsion copolymerization.The core-shell morphology of the microspheres was shown by scanning electron microscopy and transmission electron microscopy.Rabbit immunoglobulin G (as antigen) was covalently coupled onto the microspheres by the reaction between succinimide-activated ester groups on the shell of the microspheres and amino groups of the antigen molecules.The size of particles was characterized by dynamic light scattering technique and was found to vary upon bioconjugation and interaction with proteins.The binding process was shown to be specific to goat anti-rabbit immunoglobulin G(as antibody) and reversible upon the addition of free antigen into the system.     

镀锌扁钢电力接地材料在NaCl介质中的腐蚀特性

应用极化曲线、电化学阻抗谱和中性盐雾腐蚀试验法,研究电力接地材料镀锌扁钢在5%NaCl溶液中的腐蚀行为,扫描电子显微镜（SEM）、X射线衍射仪（XRD）观察与表征镀锌扁钢样品腐蚀的表面形貌及其产物性质.结果表明,在20～60℃温度范围内,镀锌扁钢腐蚀电流随温度升高而增大,60℃时腐蚀速率达到0.8408mA.cm-2（...     

Accurate measurement method of the thickness of a quartz pendulous reed by combining polarized reflectance and vision image

The detection of thickness provides important information on the production process of the quartz pendulous reed (QRP). Through real-time detection of QPR thickness, high-quality products can be produced, and defective products can be identified and removed. In order to improve detection accuracy and efficiency, a novel noncontact method for measuring the QPR thickness by combining the polarized reflectance and vision image is presented, which can be automated. Based on Snell’s law of fundamental optics, the image of the laser spot is obtained by a laser vision system. The selection criterion of the laser incident angle is analyzed. An improved edge detection algorithm is proposed to locate the subpixel edge of the laser spot, and the calculation method of the laser spot center is presented based on a set of subpixel edge-point data obtained by defining an error function which is to optimize the distribution of data points and minimize it. Experimental results show that the method proposed has good stability and high measurement accuracy, which can achieve noncontact accurate measurement of QPR thickness.     

结构动力问题的高精度组合差分时程积分法

基于Taylor级数展开得到位移和加速度的中心差分格式,并结合速度的后差分格式,构造了一种求解结构动力问题的组合差分格式的时程积分算法,该算法为自起步的两步高精度算法。通过求解递推格式的传递矩阵及其特征值,对该算法的稳定性和精度进行了理论分析,结果表明,本文提出的算法虽属条件稳定,但其精度极高,具有周期延长率小、没有振幅衰减等优点。数值分析结果也证明本文提出的算法具有较高精度。     

兰州地区光强分布及其起伏特性研究

在兰州地区夜间进行了链路长为610m的激光传输实验,研究了高斯激光在不同天气条件下的光强分布及其起伏特性.采用三维伪彩色变换方法,分析了光斑光强的空间分布规律,得到了光斑主瓣光强服从类高斯分布,并按照晴天、多云天、阴天的顺序,光斑几何中心位置附近光强分布的陡峭度依次减小,而其衰减则依次增大的结论.同时,利用实测光强值分析了大气闪烁指数,结果表明:晴天、雨后晴天和阴天下的闪烁指数分别为0.225 4、0.189 2、0.188 8,这说明晴天下的光强起伏大于雨后晴天和阴天的,且均为弱起伏.通过对归一化光强的频数分布进行非线性拟合得到了光强的概率密度分布,它们均服从对数正态分布,其中晴天下的概率密度曲线的拟合优度更是达到了0.997 50.     

ICP-OES法测定内蒙古饮茶型氟中毒病区人发、人尿中多种生命元素

应用等离子体发射光谱仪(ICP-OES)法测定了内蒙古饮茶型氟中毒病区锡林浩特市有长期饮砖茶习惯的蒙古族牧民以及对照组(不饮砖茶的汉族居民)的发和尿中Ca, Mg, K, Al, P, Cu, Zn, Fe等八种生命元素的含量。标准物质测定结果满意,方法精密度介于2.32%到8.03%之间。通过人发和人尿样品的测定,发现长期饮砖茶人群的发铝、发镁、发钾明显高于对照组(P<0.05),尿铝、尿钙也显著高于对照组,而尿钾低于对照组(P<0.05)。表明长期饮用砖茶会影响体内Al和Ca等生命元素的代谢,其机理需进一步深入研究。     

三种TATP-铜(Ⅱ)-L-氨基酸配合物的SOD活性及其电化学性质研究

超氧化物歧化酶(SOD)广泛存在于人、动物、植物、藻类以及原核生物中,它能够催化超氧阴离子自由基发生歧化反应,平衡机体内的氧自由基,是一切生物体内重要的抗活性氧毒害的酶[1].     

Emerson试剂分光光度法测定头孢羟氨苄

采用Emerson试剂分光光度法测定头孢羟氨苄的含量。在pH 9.60的四硼酸钠-氢氧化钠缓冲溶液中,在铁氰化钾存在下,头孢羟氨苄可与4-氨基安替比林发生反应,生成红色的化合物,组成比为1比1,最大吸收波长为508nm。头孢羟氨苄的质量浓度在1.20~110.0mg·L-1范围内与其吸光度呈线性关系,方法的检出限(3s/k)为0.80mg·L-1。方法应用于头孢羟氨苄胶囊中头孢羟氨苄含量的测定,测定结果与药典法测定值相符,测定值的相对标准偏差(n=5)在0.20%~0.71%之间。Emerson试剂与头孢羟氨苄的反应机理被初步探讨。     

对交流通路中理想直流电压源相当于交流短路问题的一种阐述方法

通过对微变等效电路的分析,对交流通路中理想直流电压源相当于交流短路的问题,用比较容易理解的方法进行了阐述.     

CdSe/ZnSe/ZnS多壳层结构量子点的制备与表征

展示了一种简捷的多壳层量子点合成路线。在含有过量Se源的CdSe体系中直接注入Zn源,"一步法"合成了CdSe/ZnSe量子点;进一步以CdSe/ZnSe为"核",表面外延生长ZnS壳层制备了核/壳/壳结构CdSe/ZnSe/ZnS量子点。相对于以往报道的多壳层结构量子点的制备方法,该方法通过减少壳层的生长步骤有效地简化了实验操作,缩短了实验周期,同时减少对原料的损耗。对量子点进行高温退火处理,能够大幅提高CdSe/ZnSe/ZnS量子点的发光量子产率。透射电镜、XRD以及光谱研究表明:所制备的量子点接近球形,核与壳层纳米晶均为闪锌矿结构,最终获得的CdSe/ZnSe/ZnS量子点的光致发光量子产率达到53％。为了实现量子点的表面生物功能化,通过巯基酸进行了表面配体交换修饰,使量子点表面具有水溶性的羧基功能团,并且能够维持较高的光致发光量子产率。