Enumeration of total yeasts and molds in foods by the SimPlate Yeast and Mold-Color Indicator method and conventional culture methods: collaborative study

The relative effectiveness of the SimPlate Yeast and Mold-Color Indicator method (Y&M-CI) was compared to the U.S. Food and Drug Administration's (FDA) Bacteriological Analytical Manual (BAM) method and the proposed International Organization for Standardization (ISO) method, ISO/CD 21527, for enumerating yeasts and molds in foods. Test portions were prepared and incubated according to the conditions stated in both the BAM and ISO methods. Six food types were analyzed: frozen corn dogs, nut meats, frozen fruits, cake mix, cereal, and fresh cheese. Nut meats, frozen fruits, and fresh cheese were naturally contaminated. All other foods were artificially contaminated with either a yeast or mold. Seventeen laboratories throughout North America and Europe participated in the study. Three method comparisons were conducted. In general, there was <0.3 mean log count difference in recovery between the SimPlate method and the 2 corresponding reference methods. Moreover, mean log counts between the 2 reference methods were also very similar. The repeatability (Sr) and reproducibility (SR) standard deviations were comparable between the 3 method comparisons. These results indicate that the BAM method and the SimPlate method are equivalent for enumerating yeast and mold populations in foods. Similarly, the SimPlate method is comparable to the proposed ISO method when test portions are prepared and incubated as defined in the proposed ISO method.     

The Extracts of Polygonum cuspidatum Root and Rhizome Block the Entry of SARS-CoV-2 Wild-Type and Omicron Pseudotyped Viruses via Inhibition of the S-Protein and 3CL Protease

COVID-19, resulting from infection by the SARS-CoV-2 virus, caused a contagious pandemic. Even with the current vaccines, there is still an urgent need to develop effective pharmacological treatments against this deadly disease. Here, we show that the water and ethanol extracts of the root and rhizome of Polygonum cuspidatum (Polygoni Cuspidati Rhizoma et Radix), a common Chinese herbal medicine, blocked the entry of wild-type and the omicron variant of the SARS-CoV-2 pseudotyped virus into fibroblasts or zebrafish larvae, with IC₅₀ values ranging from 0.015 to 0.04 mg/mL. The extracts were shown to inhibit various aspects of the pseudovirus entry, including the interaction between the spike protein (S-protein) and the angiotensin-converting enzyme II (ACE2) receptor, and the 3CL protease activity. Out of the chemical compounds tested in this report, gallic acid, a phytochemical in P. cuspidatum, was shown to have a significant anti-viral effect. Therefore, this might be responsible, at least in part, for the anti-viral efficacy of the herbal extract. Together, our data suggest that the extracts of P. cuspidatum inhibit the entry of wild-type and the omicron variant of SARS-CoV-2, and so they could be considered as potent treatments against COVID-19.     

Optical and acoustic study of nucleation and growth of bubbles at a liquid-solid interface induced by nanosecond-pulsed-laser heating

The dynamics of liquid-vapor phase-change in the nanosecond time-scale induced by pulsed-laser heating of a liquid on a solid sample is studied by means of optical reflectance and scattering measurements, and the piezoelectric detection technique. The liquids studied include water, ethanol, methanol, IsoproPropyl Alcohol (IPA), and mixtures of water and IPA. The threshold fluence for nucleation is determined with high accuracy using the optical and acoustic signals. Heat diffusion calculations performed for the threshold fluences indicate that the liquids are sufficiently superheated before nucleation sets on. The transient optical reflectance signal is analyzed by an effective-medium theory to provide bubble-growth kinetics, so that the bubble-growth velocity for the test liquids could be estimated. In addition, it is observed that, following the thermally induced nucleation, repetitive acoustic cavitation at the surface of the solid sample occurs, with a time interval related to the speed of sound in the liquid.     

Invariant ideals of twisted crossed products

We will prove a result concerning the inclusion of non-trivial invariant ideals inside non-trivial ideals of a twisted crossed product. We will also give results concerning the primeness and simplicity of crossed products of twisted actions of locally compact groups on -algebras. Received: 25 January 2002; in final form: 22 May 2002/Published online: 2 December 2002 This work is partially supported by Hong Kong RGC Direct Grant.     

Dynamic analysis of periodic structures

The natural vibration analysis of a periodic structure with repeated identical substructures may be simplified by using some symmetrical properties of the substructure dynamic matrices, resulting in a set of linear difference equations in the displacements. These equations are readily solved for cyclic symmetric systems, simply supported systems and infinite systems. The order of the overall frequency equations is at most equal to one half of the total number of degrees of freedom retained for a single substructure regardless of the number of substructures in the system. With these natural modes, the system with general boundary conditions at end stations is analyzed by a fast converging method.