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21.
Emrys A. Jones Nicholas P. Lockyer John C. Vickerman 《International journal of mass spectrometry》2007,260(2-3):146
Recent developments in desorption/ionisation mass spectrometry techniques have made their application to biological analysis a realistic and successful proposition. Developments in primary ion source technology, mainly through the advent of polyatomic ion beams, have meant that the technique of secondary ion mass spectrometry (SIMS) can now access the depths of information required to allow biological imaging to be a viable option.Here the role of the primary ion C60+ is assessed with regard to molecular imaging of lipids and pharmaceuticals within tissue sections. High secondary ion yields and low surface damage accumulation are demonstrated on both model and real biological samples, indicating the high secondary ion efficiency afforded to the analyst by this primary ion when compared to other cluster ion beams used in imaging. The newly developed 40 keV C60+ ion source allows the beam to be focused such that high resolution imaging is demonstrated on a tissue sample, and the greater yields allow the molecular signal from the drug raclopride to be imaged within tissue section following in vivo dosing.The localisation shown for this drug alludes to issues regarding the chemical environment affecting the ionisation probability of the molecule; the importance of this effect is demonstrated with model systems and the possibility of using laser post-ionisation as a method for reducing this consequence of bio-sample complexity is demonstrated and discussed. 相似文献
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M. C. Jones 《Annals of the Institute of Statistical Mathematics》2004,56(4):733-742
A simple new family of distributions is proposed which has support the unit disc in two dimensions. The density functions
of the family are unimodal, monotonic or uniantimodal. The bivariate symmetric beta distributions, which include the uniform
distribution, are special cases, but many members of the family are skew. The distributions have three parameters, one controlling
orientation, one controlling degree of concentration and the third controlling skewness, or more precisely off-centredness.
Importantly, these parameters are globally orthogonal. An illustrative example of fitting the model to data is given. Conditional
and marginal distributions are considered. The new distributions are compared favourably with an earlier suggestion of the
same author. 相似文献