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201.
The first extensive study of yeast metabolite GC x GC-TOFMS data from cells grown under fermenting, R, and respiring, DR, conditions is reported. In this study, recently developed chemometric software for use with three-dimensional instrumentation data was implemented, using a statistically-based Fisher ratio method. The Fisher ratio method is fully automated and will rapidly reduce the data to pinpoint two-dimensional chromatographic peaks differentiating sample types while utilizing all the mass channels. The effect of lowering the Fisher ratio threshold on peak identification was studied. At the lowest threshold (just above the noise level), 73 metabolite peaks were identified, nearly three-fold greater than the number of previously reported metabolite peaks identified (26). In addition to the 73 identified metabolites, 81 unknown metabolites were also located. A Parallel Factor Analysis graphical user interface (PARAFAC GUI) was applied to selected mass channels to obtain a concentration ratio, for each metabolite under the two growth conditions. Of the 73 known metabolites identified by the Fisher ratio method, 54 were statistically changing to the 95% confidence limit between the DR and R conditions according to the rigorous Student's t-test. PARAFAC determined the concentration ratio and provided a fully-deconvoluted (i.e. mathematically resolved) mass spectrum for each of the metabolites. The combination of the Fisher ratio method with the PARAFAC GUI provides high-throughput software for discovery-based metabolomics research, and is novel for GC x GC-TOFMS data due to the use of the entire data set in the analysis (640 MB x 70 runs, double precision floating point). 相似文献
202.
Pierce CY Barr JR Woolfitt AR Moura H Shaw EI Thompson HA Massung RF Fernandez FM 《Analytica chimica acta》2007,583(1):23-31
Accurate bacterial identification is important in diagnosing disease and in microbial forensics. Coxiella burnetii, a highly infective microorganism causative of the human disease Q fever, is now considered a U.S. category B potential bioterrorism agent. We report here an approach for the confirmatory identification of C. burnetii at the strain level which involves the combined use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and supervised pattern recognition via Partial Least Squares-Discriminant Analysis (PLS-DA). C. burnetii isolates investigated in this study included the following prototype strains from different geographical and/or historical origins and with different antigenic properties: Nine Mile I, Australian QD, M44, KAV, PAV, Henzerling, and Ohio. After culture and purification following standard protocols, linear MALDI-TOF mass spectra of pure bacterial cultures were acquired in positive ion mode. Mass spectral data were normalized, baseline-corrected, denoised, binarized and modeled by PLS-DA under crossvalidation conditions. Robustness with respect to uncontrolled variations in the sample preparation and MALDI analysis protocol was assessed by repeating the experiment on five different days spanning a period of 6 months. The method was validated by the prediction of unknown C. burnetii samples in an independent test set with 100% sensitivity and specificity for five out of six strain classes. 相似文献
203.
Pierce CY Barr JR Cody RB Massung RF Woolfitt AR Moura H Thompson HA Fernandez FM 《Chemical communications (Cambridge, England)》2007,(8):807-809
Direct analysis in real time (DART) is implemented on a time-of-flight (TOF) mass spectrometer, and used for the generation of fatty acid methyl esters (FAMEs) ions from whole bacterial cells. 相似文献
204.
All four 2',3'-dideoxy-3'-thio-nucleosides (ddtNTPs) function as substrates for the Y410F mutant of Deep Vent (exo-) DNA polymerase. Not only are the ddtNTPs incorporated to form the N + 1 product, but further elongations are observed in which the key step is attack of the 3'-thiol on the 5'-triphosphate. Although other polymerases are likely to differ in their use of the ddtNTPs, there does not appear to be a fundamental prohibition against using a thiol nucleophile on a phosphate anhydride electrophile. The syntheses of four ddtNTPs (C, T, A, G) are described. [structure: see text] 相似文献
205.
Rate constants for the gas-phase reactions of O(3P) atom with a series of monoterpenes have been determined at ambient temperature (ca. 302–309 K) and atmospheric pressure using a relative rate technique. Using the literature rate constants for O(3P) + isobutene, cis and trans-2-butene, 3-methyl-1-butene, 2-methyl-2-butene, and 2,3-dimethyl-2-butene as the standards, the O(3P) rate constants derived for the terpenes (in units of 10−11 cm3 molecule−1s −1) are 2.8 ± 0.4 for α-pinene, 2.8 ± 0.5 for β-pinene, 3.1 ± 0.5 for Δ 3-carene, 3.5 ± 0.5 for 2-carene, 2.6 ± 0.5 for camphene, 7.6 ± 1.2 for d-limonene, 9.0 ± 1.6 for γ-terpinene, and 10.7 ± 1.6 for terpinolene. The relative rate constants in this work agreed with literature values to within ± 10% for the standard alkenes, and to within ± ca. 35% for the terpenes. © 1996 John Wiley & Sons, Inc. 相似文献
206.
207.
Jean M. Bray Scott Pierce Alfredo M. Angeles-Boza Mark W. Peczuh 《Molecules (Basel, Switzerland)》2022,27(12)
We report the first total synthesis of 5-phenyl preacinetobactin and its characterization. The route was developed for the synthesis of preacinetobactin, the siderophore critical to the Gram-negative pathogen A. baumannii. It leverages a C5-substituted benzaldehyde as a key starting material and should enable the synthesis of similar analogs. 5-Phenyl preacinetobactin binds iron in a manner analogous to the natural siderophore, but it did not rescue growth in a strain of A. baumannii unable to produce preacinetobactin. 相似文献
208.
O. Macchia S. S. Leuchin J. St. Pierce W. C. Selzer und A. M. Peter 《Fresenius' Journal of Analytical Chemistry》1929,76(5-6):231-233
Ohne Zusammenfassung 相似文献
209.
Parr C Pierce SE Smith SI Brodbelt JS 《International journal of mass spectrometry》2011,304(2-3):115-123
The reactions of two well-known chemical probes, glyoxal and potassium permanganate (KMnO(4)), with oligodeoxynucleotides were monitored by electrospray ionization (ESI) mass spectrometry to evaluate the influence of the sequence of DNA, its secondary structure, and interactions with associated ligands on the reactivity of the two probes. Glyoxal, a guanine-reactive probe, incorporated a mass shift of 58 Da, and potassium permanganate (KMnO(4)) is a thymine-reactive probe that resulted in a mass shift of 34 Da. The reactions depended on the accessibility of the nucleobases, and the peak abundances of the adducts in the ESI-mass spectra were used to quantify the extent of the chemical probe reactions. In this study, both mixed-base sequences were studied as well as control sequences in which one reactive site was located at the terminus or center of the oligodeoxynucleotide while the surrounding bases were a second, different nucleobase. In addition, the reactions of the chemical probes with non-covalent complexes formed between DNA and either actinomycin D or ethidium bromide, both known to interact with single strand DNA, were evaluated. 相似文献
210.
A conjecture of Serre concerns the number of rational points of bounded height on a finite cover of projective space . In this paper, we achieve Serre?s conjecture in the special case of smooth cyclic covers of any degree when , and surpass it for covers of degree when . This is achieved by a new bound for the number of perfect r-th power values of a polynomial with nonsingular leading form, obtained via a combination of an r-th power sieve and the q-analogue of van der Corput?s method. 相似文献