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71.
Bustamante JJ Garcia M Gonzalez L Garcia J Flores R Aguilar RM Trevino A Benavides L Martinez AO Haro LS 《Electrophoresis》2005,26(23):4389-4395
A method for separating proteins with a molecular mass difference of 2 kDa using SDS-PAGE under nonreducing conditions is presented. A sample mixture containing several human growth hormone (hGH) isoforms was initially separated on a weak anion-exchange column. Fractions rich in 24 kDa hGH as determined by analytical SDS-PAGE were pooled and further separated by cation-exchange chromatography. The fractions pooled from the cation-exchange chromatography contained two hGH isoforms with a 2 kDa molecular mass difference according to SDS-PAGE analysis, 22 and 24 kDa hGH. The 22 and 24 kDa hGH were separated using continuous-elution preparative double-inverted gradient PAGE (PDG-PAGE) under nonreducing conditions. The preparative electrophoresis gel was composed of three stacked tubular polyacrylamide matrices, a 4% stacking gel, a 13-18% linear gradient gel, and a 15-10% linear inverted gradient gel. Fractions containing purified 24 kDa hGH were pooled and Western blot analysis displayed immunoreactivity to antihGH antibodies. PDG-PAGE provides researchers with an electrophoretic technique to preparatively purify proteins under nonreducing conditions with molecular mass differences of 2 kDa. 相似文献
72.
Ramón Serrano Roberto Gaxiola Gabino Ríos Javier Forment Oscar Vicente Roc Ros 《Monatshefte für Chemie / Chemical Monthly》2003,11(3):1445-1464
We have performed functional genomics of salt stress by overexpression of gene libraries in yeast and selection for salt tolerance. Thirty halotolerance genes were isolated from yeast, Arabidopsis, and sugar beet. The results indicate that Na+ transport (uptake, efflux, and compartmentation), sulfate activation, RNA processing, and protein synthesis are crucial for salt tolerance. 相似文献
73.
The conventional four-step sequential extraction method and the EDTA and acetic acid single extraction procedures were applied to sewage sludge and sediment samples. The results obtained with these samples for Cu, Cr, Ni, Pb and Zn using the Tessier method were compared with those supplied by the two single extraction procedures employed. In addition, the Tessier method was also applied to a reference material, CRM 483, and these results were also compared with the certified EDTA and acetic acid values for this sample. As a result, good agreement was found between the metal contents released in the first three fractions of the Tessier method and those leached by the simpler single extraction procedures for the most of the elements studied. Subsequently, the conventional EDTA and acetic acid extraction methods were accelerated by means of microwave energy, in order to reduce the operating time. The extraction efficiency of the first three fractions of the Tessier method was compared with that obtained using the optimised microwave single extraction procedures and only in sewage sludge and CRM 483 samples were satisfactory results found for all the elements studied, except Cr and Pb. This means that the microwave single extraction procedures optimised in this work could be employed as screening methods to evaluate rapidly the easiest mobilizable heavy metals in these samples, although more samples should be analysed to determine their general applicability. The application of the accelerated single extraction procedures to a reference material, CRM 483, provided satisfactory results for all the elements studied, except for Cr in both methods and for Pb in the acetic acid extracts. 相似文献
74.
Maria Ana Castro Zulema D. Chaia Oscar E. Piro Fabio D. Cukiernik Eduardo E. Castellano Marcia Rusjan 《Acta Crystallographica. Section C, Structural Chemistry》2002,58(7):m393-m395
The title compound, tetrakis(μ‐3,4,5‐triethoxybenzoato‐κ2O:O′)bis[(pyrazine‐κN)rhodium(II)](Rh—Rh), [Rh2(C13H17O5)4(C4H4N2)2], crystallizes on an inversion centre in the triclinic space group . The equatorial carboxylate ligands bridge the two RhII atoms, giving a binuclear lantern‐like structure. The pyrazine molecules occupy the two axial coordination sites. The phenyl rings are tilted by ca 10° with respect to the attached carboxylate groups. The pyrazine planes have a torsion angle of ca 19° around the Rh—N bond with respect to the plane of the nearer carboxylate group and are not coplanar with the Rh—Rh bond. 相似文献
75.
Matthias Tacke Lorcan T. Allen William M. Gallagher Oscar Mendoza Franz-Josef K. Rehmann 《Journal of organometallic chemistry》2004,689(13):2242-2249
Starting from 6-(p−N,N-dimethylanilinyl)fulvene (1a) or 6-(pentamethylphenyl)fulvene (1b) [1,2-di(cyclopentadienyl)-1,2-di(p−N,N-dimethylaminophenyl)ethanediyl] titanium dichloride (2a) and [1,2-di(cyclopentadienyl)-1,2-bis(pentamethylphenyl)ethanediyl] titanium dichloride (2b) and their corresponding dithiocyanato complexes (3a, 3b) were synthesized. Titanocene 2b did not show a cytotoxic effect, but when 2a was tested against pig kidney carcinoma cells (LLC-PK) or human ovarian carcinoma cells (A2780/cp70) inhibitory concentrations (IC50) of 2.7 × 10−4 and 1.9 × 10−4 M, respectively, were observed. 相似文献
76.
2-Acetoxy-3,4-di-O-acetyl-D-arabinal (6), similar to its D-xylo analogue 4, reacted with benzyl alcohol by the tin(IV) chloride-promoted glycosylation to produce optically active (S)-2-benzyloxy-2H-pyran-3(6H)-one (8a). The L-arabinal derivative (5) gave 9a, the dihydropyranone enantiomer of 8a. These results indicated that the configuration of the C-4 stereocenter in the starting glycal defines the configuration of the new chiral center in the resulting dihydropyranone. The influence of other catalysts (BF(3) or iodine) employed for the glycosylation on the optical purity of the dihydropyranone was studied. Enantiomerically pure dihydropyranones 8b and 9c were obtained using chiral alcohols ((R)- and (S)-2-octanol, respectively) as glycosylating agents. Compounds 8a,b and 9a,c proved to be reactive dienophiles in thermal and Lewis acid-promoted Diels-Alder reactions. The addition of 2,3-dimethylbutadiene, cyclopentadiene, and 1,3-cyclohexadiene to the beta-pyranones 8a,b led to the corresponding adducts 10a,b, 12a,b, and 16a,b as major products. Enantiomeric cycloadducts were synthesized from the alpha-pyranones 9a,c. The main products were formed by highly facial-diastereoselective addition of dienes to the pyranone ring, guided by the sterical hindrance of the alkoxy substituent of the C-2 stereocenter. As cycloadditions with cycloalkadienes were also highly endo diastereoselective, these reactions gave access to pure tetrahydrobenzopyranones that carry a multitude of stereogenic centers installed in a predictable way. 相似文献
77.
María L. Dell'Arciprete Carlos J. Cobos Dr. Jorge P. Furlong Dr. Daniel O. Mártire Dr. Mónica C. Gonzalez Dr. 《Chemphyschem》2007,8(17):2498-2505
The kinetics of the oxidation of pyridine, 3‐chloropyridine, 3‐cyanopyridine, 3‐methoxypyridine and 3‐methylpyridine mediated by SO4 < M ‐> radicals are studied by flash photolysis of peroxodisulphate, S2O82?, at pH 2.5 and 9. The absolute rate constants for the reactions of both, the basic and acid forms of the pyridines, are determined and discussed in terms of the Hammett correlation. The monosubstituted pyridines react about 10 times faster with sulphate radicals than their protonated forms, the pyridine ions. The organic intermediates are identified as the corresponding hydroxypyridine radical adducts and their absorption spectra compared with those estimated employing the time‐dependent density functional theory with explicit account for bulk solvent effects. A reaction mechanism which accounts for the observed intermediates and the pyridinols formed as products is proposed. 相似文献
78.
Millet O Muhandiram DR Skrynnikov NR Kay LE 《Journal of the American Chemical Society》2002,124(22):6439-6448
New pulse sequences are presented for the measurement of the relaxation of deuterium double quantum, quadrupolar order, and transverse antiphase magnetization in (13)CH(2)D methyl groups of (15)N-, (13)C-labeled, fractionally deuterated proteins. Together with previously developed experiments for measuring deuterium longitudinal and transverse decay rates [Muhandiram, D. R.; Yamazaki, T.; Sykes, B. D.; Kay, L. E. J. Am. Chem. Soc. 1995, 117, 11536], these schemes allow measurement of the five unique decay constants of a single deuteron, providing an unprecedented opportunity to investigate side-chain dynamics in proteins. All five deuterium relaxation rates have been measured for deuterons in the methyl groups of the B1 immunoglobulin binding domain of peptostreptococcal protein L and the N-terminal SH3 domain from the protein drk. Since values of the spectral density function at only three different frequencies contribute to the five relaxation rates, the self-consistency of the relaxation data is readily established. Very good agreement is obtained between calculated parameters describing the amplitudes and time scales of motion when different subsets of the relaxation data are employed. 相似文献
79.
Andrs Zelcer Zulema D. Chaia Fabio D. Cukiernik Oscar E. Piro Eduardo E. Castellano 《Acta Crystallographica. Section C, Structural Chemistry》2002,58(3):m144-m146
The title compound, catena‐poly[[tetrakis(μ‐decanoato‐κ2O:O′)diruthenium(II,III)(Ru—Ru)]‐μ‐octanesulfonato‐κ2O:O′], [Ru2(C10H19O2)4(C8H17O3S)], is an octanesulfonate derivative of the mixed‐valence complex diruthenium tetradecanoate. The equatorial carboxylate ligands are bidentate, bridging two Ru atoms to form a dinuclear structure. Each of the two independent dinuclear metal complexes in the asymmetric unit is located at an inversion centre. The octanesulfonate anion bridges the two dinuclear units through axial coordination. The alkyl chains of the carboxylate and sulfonate ligands are arranged in a parallel manner. The global structure can be seen as infinite chains of polar moieties separated by a double layer of non‐polar alkyl groups, without interdigitation of the alkyl chains. 相似文献
80.
Pérez González M Gonzalez Díaz H Molina Ruiz R Cabrera MA Ramos de Armas R 《Journal of chemical information and computer sciences》2003,43(4):1192-1199
A new application of TOPological Sub-structural MOlecular DEsign (TOPS-MODE) was carried out in herbicides using computer-aided molecular design. Two series of compounds, one containing herbicide and the other containing nonherbicide compounds, were processed by a k-Means Cluster Analysis in order to design the training and prediction sets. A linear classification function to discriminate the herbicides from the nonherbicide compounds was developed. The model correctly and clearly classified 88% of active and 94% of inactive compounds in the training set. More specifically, the model showed a good global classification of 91%, i.e., (168 cases out of 185). While in the prediction set, they showed an overall predictability of 91% and 92% for active and inactive compounds, being the global percentage of good classification of 92%. To assess the range of model applicability, a virtual screening of structurally heterogeneous series of herbicidal compounds was carried out. Two hundred eighty-four out of 332 were correctly classified (86%). Furthermore this paper describes a fragment analysis in order to determine the contribution of several fragments toward herbicidal property; also the present of halogens in the selected fragments were analyzed. It seems that the present TOPS-MODE based QSAR is the first alternate general "in silico" technique to experimentation in herbicides discovery. 相似文献