Human pre-interleukin 1 alpha and beta: structural features revealed by limited proteolysis

Both pre-interleukin 1 alpha and beta (pre IL 1 alpha and beta) are proteolytically processed into extracellular mature forms of IL 1 alpha and beta. Since pre IL 1 alpha is shown to be biologically active, there may be other reasons for the proteolytic processing of IL 1 alpha and presumably, for IL 1 beta also. In order to examine the possibility that structural stabilization may be associated with the proteolytic processing of pre IL 1 alpha and beta, we investigated the structural features of pre IL 1 alpha and beta by the combination of limited proteolysis and immunoprecipitation with antibodies to the NH2-terminal halves or COOH-terminal halves of pre IL 1 alpha or beta. Both trypsin and V8 protease digested the NH2-terminal halves of pre IL 1 alpha and beta more easily than the COOH-terminal halves of pre IL 1 alpha and beta, yielding structurally stabilized "mature" forms of IL 1. Both trypsin and V8 protease yielded a fragment similar in size to mature IL 1 alpha from pre IL 1 alpha. In contrast, trypsin digested pre IL 1 beta into fragments smaller in size than mature IL 1 beta, while V8 protease yielded a fragment similar in size to mature IL 1 beta. Furthermore, mature IL 1 beta, once processed and released from cells, was resistant to trypsin.(ABSTRACT TRUNCATED AT 250 WORDS)     

Fluorocarbon derivatives of nitrogen. Part I. Some reactions of perfluoro-1-azacyclohexene

Controlled displacement of fluorine from perfluoro-1-azacyclo-hexene (I) by the nucleophilic reagents Me₂NH, Et₂NH, $\text{CH}{}_2\text{CH}{}_2\text{O(CH}{}_2\text{)}{}_2\text{N}$H, C₆Cl₅ONa, and (CF₃)₂NONa provides the derivatives (II) - (IV), respectively. The last of these can also be obtained by treatment of the parent compound (I) with mercury^II bistrifluoromethylnitroxide.     

Solid-State Investigation,Storage, and Separation of Pyrophoric PH3 and P2H4 with α-Mg Formate

Phosphane, PH₃—a highly pyrophoric and toxic gas—is frequently contaminated with H₂ and P₂H₄, which makes its handling even more dangerous. The inexpensive metal–organic framework (MOF) magnesium formate, α-[Mg(O₂CH)₂], can adsorb up to 10 wt % of PH₃. The PH₃-loaded MOF, PH₃@α-[Mg(O₂CH)₂], is a non-pyrophoric, recoverable material that even allows brief handling in air, thereby minimizing the hazards associated with the handling and transport of phosphane. α-[Mg(O₂CH)₂] further plays a critical role in purifying PH₃ from H₂ and P₂H₄: at 25 °C, H₂ passes through the MOF channels without adsorption, whereas PH₃ adsorbs readily and only slowly desorbs under a flow of inert gas (complete desorption time≈6 h). Diphosphane, P₂H₄, is strongly adsorbed and trapped within the MOF for at least 4 months. P₂H₄@α-[Mg(O₂CH)₂] itself is not pyrophoric and is air- and light-stable at room temperature.