Ca(2+)-triggered coelenterazine-binding protein from Renilla as an enzyme-dependent label for binding assay

The recombinant Ca²⁺-triggered coelenterazine-binding protein (CBP) from Renilla muelleri was investigated as a biospecifically labeled molecule for in vitro assay applications. The protein was shown to be stable in solutions in the frozen state, as well as stable under heating and to chemical modifications. Conjugates with biotin, oligonucleotide, and proteins were obtained and applied as biospecific molecules in a solid-phase microassay. CBP detection was performed with intact (no modifications were made) Renilla luciferase in the presence of calcium, and the detection limit was found to be 75 amol. Model experiments indicate that this approach shows much promise, especially with regard to the development of multianalytical systems.     

Automaton transformations and monadic theories of infinite sequences

In this paper we prove that the set of degrees of asynchronously automaton transformations of infinite sequences with a solvable monadic theory forms an initial segment in the set of degrees of asynchronously automaton transformations. We prove a solvability criterion for a monadic theory of a complete sequence.     

Degrees of asynchronously automaton transformations

In this paper we study a partially ordered set of degrees of asynchronously automaton transformations. We prove the existence of a continuum of atoms in this set, the embeddability of every finite linearly ordered set as an initial segment in it, and the nonextensibility of an embedding of partially ordered sets.     

Polymeric gold(I) diisobutyl dithiophosphate, [Au2{S2P(O-iso-C4H9)2}2]n: Synthesis,supramolecular self-organization (a role of aurophilic interaction), 13C and 31P MAS NMR spectroscopy,and thermal behavior

A new polymeric gold(I) diisobutyl dithiophosphate (Dtph), [Au₂{S₂P(O-iso-C₄H₉)₂}₂] _n (I), was preparatively obtained and characterized by ¹³C and ³¹P MAS NMR spectroscopy and X-ray diffraction (CIF file CCDC no. 977818). Diagrams of the χ² statistic were constructed from the complete ³¹P MAS NMR spectra and used to calculate the ³¹P chemical shift anisotropy (δ _aniso = δ _zz ? δ _iso ) and the asymmetry parameter η = (δ _yy ? δ _xx )/(δ _zz ? δ _iso ). The main structural unit of complex I is the noncentrosymmetric dinuclear molecule [Au₂{S₂P(O-iso-C₄H₉)₂}₂], in which the gold atoms are linked by two bridging ligands Dtph. The central cyclic structural fragment of the dimer [Au₂S₄P₂] is additionally stabilized by the intramolecular aurophilic interaction Au?Au. Further supramolecular self-organization of the complex involves intermolecular aurophilic bonds Au?Au that serve to unite adjacent dinuclear molecules [Au₂{S₂P(O-iso-C₄H₉)₂}₂] with different spatial orientations into the polymer chains ([Au₂{S₂P(O-iso-C₄H₉)₂}₂]) _n . The thermal behavior of complex I was examined by synchronous thermal analysis under argon. The character of the thermolysis of the complex to reduced metallic gold as a final product was determined.     

A computerized complex for recording and processing of reflected high-energy electron diffraction patterns

An efficient and high-speed complex for visualization, reading, and processing of reflected high-energy electron diffraction patterns is described. The block diagrams and technical data of the complex, as well as its interface with devices controlling molecular-beam epitaxy of semiconductor structures, are presented. Dedicated software for processing high-energy electron diffraction patterns, including in real time, is suggested. The complex was used for studying heteroepitaxial growth and the formation of InAs nanostructures on the GaAs and Si surfaces.