Fluorescence lifetime imaging with a low-repetition-rate passively mode-locked diode-pumped Nd:YVO4 oscillator

We report a wide-field fluorescence lifetime imaging microscope based on a low-repetition-rate (3.7-MHz) passively mode-locked diode-pumped laser source. This inexpensive and compact laser source operating in the visible and UV range can excite a wide range of fluorophores of biological interest. We demonstrate that the power of this laser source is highly sufficient for studying biological systems with low quantum yields (autofluorescence of tissues and stained living cells). The maximum measurable lifetime is also strongly increased with this laser source, as fluorescence intensity measurement can occur 250 ns after the excitation pulse.     

The 19F and 31P magnetic resonance spectra of phosphorus trifluoride in nematic solution

The phosphorus and fluorine N.M.R. spectra of PF₃ in a nematic solvent (EBBA) are discussed. The molecular geometry is found to agree with structures obtained from microwave and electron diffraction studies. The sign of the ordering parameter is assumed negative, the signs of the phosphorus fluorine coupling constant and of the chemical shift anisotropies follow from this assumption, and are found to be in agreement with theoretical predictions. The effect of temperature on the isotropic chemical shift of fluorine is discussed.     

Coupled horizontal and vertical bending vibrations of a stationary shaft with two cracks

This paper investigates the coupled bending vibrations of a stationary shaft with two cracks. It is known from the literature that, when a crack exists in a shaft, the bending, torsional, and longitudinal vibrations are coupled. This study focuses on the horizontal and vertical planes of a cracked shaft, whose bending vibrations are caused by a vertical excitation, in the clamped end of the model. When the crack orientations are not symmetrical to the vertical plane, a response in the horizontal plane is observed due to the presence of the cracks. The crack orientation is defined by the rotational angle of the crack, a parameter which affects the horizontal response. When more cracks appear in a shaft, then the coupling becomes stronger or weaker depending on the relative crack orientations. It is shown that a double peak appears in the vibration spectrum of a cracked or multi-cracked shaft.Modeling the crack in the traditional manner, as a spring, yields analytical results for the horizontal response as a function of the rotational angle and the depths of the two cracks. A 2×2 compliance matrix, containing two non-diagonal terms (those responsible for the coupling) serves to model the crack. Using the Euler–Bernoulli beam theory, the equations for the natural frequencies and the coupled response of the shaft are defined. The experimental coupled response and eigenfrequency measurements for the corresponding planes are presented. The double peak was also experimentally observed.     

Free-space fluorescence molecular tomography utilizing 360 degrees geometry projections

Fluorescence tomography of diffuse media can yield optimal three-dimensional imaging when multiple projections over 360 degrees geometries are captured, compared with limited projection angle systems such as implementations in the slab geometry. We demonstrate how it is possible to perform noncontact, 360 degrees projection fluorescence tomography of mice using CCD-camera-based detection in free space, i.e., in the absence of matching fluids. This approach achieves high spatial sampling of photons propagating through tissue and yields a superior information content data set compared with fiber-based 360 degrees implementations. Reconstruction feasibility using 36 projections in 10 degrees steps is demonstrated in mice.     

Pulsed-field gradient nuclear magnetic resonance study of transport properties of fluid catalytic cracking catalysts

Pulsed-field gradient nuclear magnetic resonance (PFG NMR) has been applied to study molecular diffusion in industrial fluid catalytic cracking (FCC) catalysts and in USY zeolite for a broad range of molecular displacements and temperatures. The results of this study have been used to elucidate the relevance of molecular transport on various displacements for the rate of molecular exchange between catalyst particles and their surroundings. It turned out that this rate, which may determine the overall rate and selectivity of FCC process, is primarily related to the diffusion mode associated with displacements larger than the size of zeolite crystals located in the particles but smaller than the size of the particles. This conclusion has been confirmed by comparative studies of the catalytic performance of different FCC catalysts.     

Stretcher-free high energy nonlinear amplification of femtosecond pulses in rod-type fibers

We report on the study of direct amplification of femtosecond pulses in an 80 mum core diameter microstructured Yb-doped rod-type fiber amplifier in the nonlinear regime. The system includes a compact single grating compressor for the compensation of the small dispersion in the amplifier. With a 1250 line/mm (l/mm) grating-based compressor, pulses as short as 49 fs with 870 nJ pulse energy and 12 MW peak power are obtained. Alternatively, the use of a 1740 l/mm grating allows the production of higher quality pulses of 70 fs, 1.25 microJ pulse energy, and 16 MW peak power.     

Temporal cleaning of a high-energy fiber-based ultrafast laser using cross-polarized wave generation

We report the use of cross-polarized wave generation to perform both pulse shortening and temporal cleaning of a high-energy ytterbium-doped fiber-based femtosecond laser system. The nonlinear processes allow both a highly efficient nonlinear conversion of 20% and a large compression ratio of 3.5, with inherently improved coherent and incoherent contrasts. This results in the generation of 37 μJ, 115 fs pulses at a repetition rate of 100 kHz with high temporal quality.     

Cross sections for multi-particle final states at a linear collider

In this paper total cross sections for signals and backgrounds of top- and Higgs-production channels in e ⁺ e^- collisions at a future linear collider are presented. All channels considered are characterized by the emergence of six-particle final states. The calculation takes into account the full set of tree-level amplitudes in each process. Two multi-purpose parton level generators, HELAC/PHEGAS and AMEGIC + + , are used, and their results are found to be in perfect agreement.Received: 26 November 2003, Revised: 15 January 2004, Published online: 3 March 2004