全文获取类型
收费全文 | 244篇 |
免费 | 8篇 |
专业分类
化学 | 178篇 |
晶体学 | 3篇 |
力学 | 7篇 |
数学 | 31篇 |
物理学 | 33篇 |
出版年
2024年 | 1篇 |
2023年 | 7篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 12篇 |
2015年 | 6篇 |
2014年 | 14篇 |
2013年 | 21篇 |
2012年 | 23篇 |
2011年 | 27篇 |
2010年 | 11篇 |
2009年 | 11篇 |
2008年 | 21篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 7篇 |
2004年 | 10篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1995年 | 1篇 |
排序方式: 共有252条查询结果,搜索用时 781 毫秒
31.
Nikhil Dube Andrew D. Presley Jessica Y. Shu Ting Xu 《Macromolecular rapid communications》2011,32(4):344-353
Polymers conjugated to the exterior of a protein mediate its interactions with surroundings, enhance its processability and can be used to direct its macroscopic assemblies. Most studies to date have focused on peptide–polymer conjugates based on hydrophilic polymers. Engineering amphiphilicity into protein motifs by covalently linking hydrophobic polymers has the potential to interface peptides and proteins with synthetic polymers, organic solvents, and lipids to fabricate functional hybrid materials. Here, we synthesized amphiphilic peptide–polymer conjugates in which a hydrophobic polymer is conjugated to the exterior of a heme‐binding four‐helix bundle and systematically investigated the effects of the hydrophobicity of the conjugated polymer on the peptide structure and the integrity of the heme‐binding pocket. In aqueous solution with surfactants present, the side‐conjugated hydrophobic polymers unfold peptides and may induce an α‐helix to β‐sheet conformational transition. These effects decrease as the polymer becomes less hydrophobic and directly correlate with the polymer hydrophobicity. Upon adding organic solvent to solubilize the hydrophobic polymers, however, the deleterious effects of hydrophobic polymers on the peptide structures can be eliminated. Present studies demonstrate that protein structure is sensitive to the local environment. It is feasible to dissolve amphiphilic peptide–polymer conjugates in organic solvents to enhance their solution processability while maintaining the protein structures.
32.
Orendorff CJ Gearheart L Jana NR Murphy CJ 《Physical chemistry chemical physics : PCCP》2006,8(1):165-170
Silver and gold nanorods with aspect ratios from 1 to 16 have been used as substrates for surface enhanced Raman spectroscopy (SERS) in colloidal solution. The nanorod aspect ratio is varied to give different degrees of overlap between the nanorod longitudinal plasmon band and excitation source in order to determine its effect on overall surface enhancement. Results suggest that enhancement factors are a factor of 10-10(2) greater for substrates that have plasmon band overlap with the excitation source than for substrates whose plasmon bands do not. 相似文献
33.
Chakraborty A Kar S Nath DN Guchhait N 《The journal of physical chemistry. A》2006,110(44):12089-12095
A donor-acceptor substituted aromatic system (E)-3-(4-Methylamino-phenyl)-acrylic acid methyl ester (MAPAME) has been synthesized, and its photophysical behavior obtained spectroscopically has been compared with the theoretical results. The observed dual fluorescence from MAPAME has been assigned to emission from locally excited and twisted intramolecular charge transfer states. The donor and acceptor angular dependency on the ground and excited states potential energy surfaces have been calculated both in vacuo and in acetonitrile solvent using time dependent density functional theory (TDDFT) and TDDFT polarized continuum model (TDDFT-PCM), respectively. Calculation predicts that a stabilized twisted excited state is responsible for red shifted charge transfer emission. 相似文献
34.
35.
A logarithmic signature (LS) for a finite group G is an ordered tuple α = [A 1, A 2, . . . , A n ] of subsets A i of G, such that every element ${g \in G}$ can be expressed uniquely as a product g = a 1 a 2 . . . a n , where ${a_i \in A_i}$ . The length of an LS α is defined to be ${l(\alpha)= \sum^{n}_{i=1}|A_i|}$ . It can be easily seen that for a group G of order ${\prod^k_{j=1}{p_j}^{m_j}}$ , the length of any LS α for G, satisfies, ${l(\alpha) \geq \sum^k_{j=1}m_jp_j}$ . An LS for which this lower bound is achieved is called a minimal logarithmic signature (MLS) (González Vasco et al., Tatra Mt. Math. Publ. 25:2337, 2002). The MLS conjecture states that every finite simple group has an MLS. This paper addresses the MLS conjecture for classical groups of Lie type and is shown to be true for the families PSL n (q) and PSp 2n (q). Our methods use Singer subgroups and the Levi decomposition of parabolic subgroups for these groups. 相似文献
36.
Haridas J. Kharat Kishor P. Kakde Dhammanand J. Shirale Vikas K. Gade Pradeep D. Gaikwad Padmakar A. Savale 《Fiber and Integrated Optics》2013,32(6):411-422
Evanescent wave configuration has been extensively used in the development of fiber-optic sensor for different biomedical applications. In the present investigation we have theoretically proposed the designing of single-mode and multimode optical fiber sensing probes, which can be prepared by removing a few centimeters of cladding near the distal end of the step index optical fiber. In this theoretical study, we found that the removal of cladding causes V-number mismatching and this has led to the loss of signal acquisition from the sensing region. Therefore, to minimize these losses we have proposed to reduce the radius by step etch and tapering technique. It was found that step etching and tapering enhances the strength and penetration depth of the evanescent wave significantly. 相似文献
37.
38.
39.
Balia Singh R Mahanta S Guchhait N 《Journal of photochemistry and photobiology. B, Biology》2008,91(1):1-8
In the present work, we have studied the interaction of proton transfer probe 1-hydroxy-2-naphthaldehyde (HN12) with Human Serum Albumin (HSA) and Bovine Serum Albumin (BSA) by steady state absorption and emission spectroscopy combined with time resolved fluorescence measurements. The measured binding constant (K) and free energy change (DeltaG) indicate a stronger affinity of HN12 molecule for HSA than BSA. Steady state anisotropy, excitation anisotropy and fluorescence resonance energy transfer (FRET) studies indicate that the probe molecule resides at the hydrophobic site of the protein environment. 相似文献
40.
Earhart C Jana NR Erathodiyil N Ying JY 《Langmuir : the ACS journal of surfaces and colloids》2008,24(12):6215-6219
Nanoparticle-based probes are emerging as alternatives to molecular probes due to their various advantages, such as bright and tunable optical property, enhanced chemical and photochemical stability, and ease of introduction of multifunctionality. This work presents a simple and general approach for functionalizing various nanoparticle systems for use as glycobiological probes. Silica-coated nanoparticles of Ag, Fe3O4, and ZnS-CdSe were synthesized and functionalized with dextran. The resulting 10-40-nm-sized particles were robust, water-soluble, colloidally stable, and biochemically active. 相似文献