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11.
Harroun TA Koslowsky M Nieh MP de Lannoy CF Raghunathan VA Katsaras J 《Langmuir : the ACS journal of surfaces and colloids》2005,21(12):5356-5361
Mixtures of long- and short-chain phospholipids, specifically 14:0 and 6:0 phosphatidylcholines (DMPC and DHPC), have been used successfully in NMR studies as magnetically alignable substrates for membrane-associated proteins. However, recent publications have shown that the phase behavior of these mixtures is much more complex than originally thought. Using polarized light microscopy and small-angle neutron scattering, phase diagrams of DMPC/DHPC mixtures at molar ratios of 2, 3.2, and 5 have been determined. Generally, at temperatures below the main-chain melting transition of DMPC (T(M) = 23 degrees C), an isotropic phase of disk-like micelles is found. At high temperatures (T > 50 degrees C), a lamellar phase consisting of either multilamellar vesicles (MLV) or extended lamellae is formed, which at low lipid concentrations (e.g., MLV) coexists with an excess of water. At intermediate temperatures and lipid concentrations, a chiral nematic phase made up of worm-like micelles was observed. 相似文献
12.
Kucerka N Papp-Szabo E Nieh MP Harroun TA Schooling SR Pencer J Nicholson EA Beveridge TJ Katsaras J 《The journal of physical chemistry. B》2008,112(27):8057-8062
The asymmetric outer membrane of Gram-negative bacteria contains lipopolysaccharides (LPSs) which contribute significantly to the bacterium's surface properties and play a crucial role in regulating membrane permeability. We report on neutron diffraction studies performed on aligned, self-assembled bilayers of Na-, Ca-, and Mg-salt forms of LPS isolated from Pseudomonas aeruginosa PAO1. From the one-dimensional neutron scattering length density profiles we find that water penetrates Ca2+-LPS bilayers to a lesser extent than either Na+- or Mg2+-LPS bilayers. This differential water penetration could have implications as to how small molecules permeate the outer membrane of Gram-negative bacteria and, possibly, how nonlamellar phases are formed. 相似文献
13.
Conservative methods for the Toda lattice equations 总被引:1,自引:0,他引:1
We are concerned with the numerical integration of the Todalattice equations by using different conservative methods. Numericalexperiments suggest that the global error for isospectral schemesdecreases exponentially with time but it is almost constantfor either symplectic or more general integrators. We providea theoretical explanation for these experimental findings. 相似文献
14.
Baohua Yue Chien-Yueh Huang Mu-Ping Nieh Charles J. Glinka John Katsaras 《Macromolecular Symposia》2005,219(1):123-134
Unilamellar vesicles (ULV) consisting of a single lipid bilayer are of special interest as drug delivery vehicles. Here, we report on a spontaneously forming ULV system composed of the short- and long-chain phospholipids, dihexanoyl (DHPC) and dimyristoyl (DMPC) phosphorylcholine, respectively, doped with the negatively charged lipid, dimyristoyl phosphorylglycerol (DMPG). Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) were employed to systematically investigate the effects of lipid concentration, salinity, and time on vesicle stability. It is found that ULV size is practically constant over a range of lipid concentration and temperature. The spontaneously formed ULV are stable for periods of four months, or greater, without the use of stabilizers. 相似文献
15.
Nieh MP Pencer J Katsaras J Qi X 《Langmuir : the ACS journal of surfaces and colloids》2006,22(26):11028-11033
We have observed a bimodal distribution of ellipsoidal unilamellar vesicles (ULVs) in a phospholipid mixture composed of dioleoyl phosphatidylserine (DOPS) and dipalmitoyl and dihexanoyl phosphatidylcholine, DPPC and DHPC, respectively. Dynamic light scattering and transmission electron microscopy data indicate a bimodal size distribution of these nanoparticles with hydrodynamic radii of approximately 200 and >500 nm, while small-angle neutron scattering data were fit using a model of coexisting monodisperse morphologies, namely, oblate and triaxial ellipsoidal vesicles. Unlike DOPS ULV formed by sonication, which can fuse days after being formed, these ULVs are stable over a period of 12 months at 4 degrees C. We also report on the structure of these ULVs associated with the two helical peptide domains (H1 and H2) of a glucosylprotein, namely, Saposin C, to gain some insight into protein-membrane interactions. 相似文献
16.
A robust and uniform porphysome, which reveals an efficient photodynamic therapy and contrast-enhanced ultrasonic imaging for theranostic applications, can be fabricated from a 4-armed porphyrin-polylactide conjugate. 相似文献
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