Interference from arsenate when determining phosphate by the malachite green spectrophotometric method

The effect of arsenate on phosphate determination by the malachite green spectrophotometric method was investigated. The molar absorptivities of the molybdophosphate and malachite green–molybdoarsenate species at 625 nm and a final acidity of 0.38 M were calculated as 10.4±0.13×10⁴ and 7.2±0.17×10⁴ l mol⁻¹ cm⁻¹ respectively, indicating that arsenate could interfere in phosphate measurement. Arsenate concentrations as low as 23 μg l⁻¹ caused increase in colour development in phosphate solutions. However, the extent of colour development for both anions depended on the final acid concentration of the solution. An acidified sodium sulphite solution (0.83 M NaSO₃, 0.83 M H₂SO₄) quantitatively prevented arsenate colour development up to 300 μg l⁻¹ As(V). It was also demonstrated that the method removed As(V) interferences in mixed As/P solutions and therefore can be used to treat natural water samples with elevated arsenate concentrations before phosphate measurement.     

Sustainable acoustic absorbers from the biomass

There is currently considerable interest in developing sustainable absorbers, either from biomass materials or recycled materials, and it is the former that is the subject of this paper. A number of potential candidate materials are available from the biomass in the form of organic fibres. Non-fibrous materials, such as configurations of whole straw or reed, can also act as sound absorbers. A combination of impedance tube and reverberation chamber measurements have been carried out for a number of biomass materials and the effectiveness of current models for the prediction of the absorptive properties of natural fibres has been investigated. Examination of the acoustical characteristics of a range of natural fibres has confirmed their effectiveness as porous sound absorbers and also the limitations of current models for predicting their performance. Examination of the acoustical performance of materials consisting of different configurations of whole reeds and straws has revealed that these also possess considerable potential for application as broadband sound absorbers with particularly good low frequency absorption characteristics. The combination of natural fibres and whole reeds offer the possibility of developing a range of sustainable absorbers which act very effectively across the complete audio frequency range.     

Synthesis of functional monolayer surfaces for rapid radiometric determination of plutonium

Glass or silicon substrates functionalized with a monolayer of carbamoylmethylphosphonate (CMP) ligands effectively bind tetravalent actinides from optimized mineral acid solutions to enable rapid, high quality radiometric assay by alpha spectrometry. The observed alpha spectra compare favorably with the highest quality electroplated samples. The CMP-functionalized surfaces have been used to develop simplified analytical methods to determine plutonium from complex mixtures.     

Studies on ternary metallo-β lactamase-inhibitor complexes using electrospray ionization mass spectrometry

Metallo-beta-lactamases (MBLs) are targets for medicinal chemistry as they mediate bacterial resistance to beta-lactam antibiotics. Electrospray-ionization mass spectrometry (ESI-MS) was used to study the inhibition by a set of mercaptocarboxylates of two representative MBLs with different optimal metal stoichiometries for catalysis. BcII is a dizinc MBL (Class B1), whilst the CphA MBL (Class B2) exhibits highest activity with a single zinc ion in the active site. Experimental parameters for the detection of the metallo-enzyme and the metallo-enzyme-inhibitor complexes were evaluated and optimized. Following investigations on the stoichiometry of metal binding, the affinity of the inhibitors was investigated by measuring the relative abundance of the complex compared to the metalloprotein. The results for the BcII enzyme were in general agreement with solution assays and demonstrated that the inhibitors bind to the dizinc form of the BcII enzyme. The results for the CphA(ZnII) complex unexpectedly revealed an increased affinity for the binding of a second metal ion in the presence of thiomandelic acid. The results demonstrate that direct ESI-MS analysis of enzyme:inhibitor complexes is a viable method for screening inhibitors and for the rapid assay of the enzyme:metal:inhibitor ratios.     

A Green function for the equilateral triangle

Using the image method, a diffusional Green function has been derived analytically for a triangle, when its three sides, all of equal length, have zero intensity. Because many image sites are involved, computational assistance is employed both to construct the Green function and to use it in solving practical problems. An alternative procedure based on eigenfunctions is also summarized.     

Radionuclides in redistributed sediments and ash from the Las Conchas fire in Northern New Mexico

The 2011 Las Conchas fire burned 632 km² of ponderosa pine forests and piñon–juniper woodlands in the Jemez Mountains in north central New Mexico. In the weeks following the fire, heavy rainfall caused extensive flooding and erosion of surface soil and ash from the affected areas. Samples from mud and ash flows were collected and analyzed for ¹³⁷Cs and Pu, which were originally deposited as global fallout during the era of atmospheric nuclear testing. The mean concentrations for ²³⁸Pu, ^239,240Pu and ¹³⁷Cs were 0.18 ± 0.05, 4.16 ± 1.95, and 111 ± 56 mBq/g, respectively. ^239,240Pu and ¹³⁷Cs are significantly elevated above non-fire affected regional background levels.     

Fourier Transform Infrared Study of A C18 Modified Quartz Surface

In reversed-phase liquid chromatography the separation mechanism depends on the solute-stationary phase and solute-solvent interactions. These interactions can be studied under near-normal chromatographic conditions by total internal reflection fluorescence (TIRF) spectroscopy. Quartz crystals chemically modified with n-octadecyl-substituted-silanes, which are common derivatizing reagents used to modify chromatographic surfaces, can be used as a model stationary phase in TIRF studies. To facilitate study of the separation mechanisms using TIRF, surfaces of quartz crystals were chemically modified with silylating reagents and characterized by Fourier Transform Infrared Spectroscopy (FT-IR). Susceptibility tests of the modified surfaces were performed using protic and aprotic solvents. Siloxane bonds were formed, between the reagent and the quartz surface, at high reaction temperature in the absence of any solvent. Siloxane bond formation in the presence of organic solvent at room temperature was observed only when the crystal was previously dehydrated at high temperature.     

Development of a multi-microphone calibrator

This paper presents the theory, design, and validation of a microphone calibrator used to simultaneously calibrate the amplitudes of multiple microphones on a single probe. The probe uses four 6 mm diameter electret microphones to acquire the data needed to compute acoustic energy density. This probe has prompted the need for simultaneous, multi-microphone amplitude calibration. The calibration process simultaneously subject each microphone on the probe to the same known acoustic pressure using four equal-length, small-diameter tubes connected to a single excitation source. A reference microphone connected to a fifth tube is used to calibrate the microphones. Test results show that the calibrator can calibrate each probe microphone within ±0.5 dB up to 2 kHz, and within ±1 dB up to 4.9 Hz with a confidence level of 95%.